Alterations in Proteomic and Metabolomic Profile in Animals Exposure to 2,3-Butanedione: A Flavoring Widely Used in the Food Industry

Jedlicka, Letícia Dias Lima; Lacerda, José Thalles Jocelino Gomes de; Neto, Giuseppe Bruno; Balbino, Aleksandro Martins; Leite, Fernndo Brunalle Vilela de Moura; Fernandes, Liliam; Assunção, Nilson Antônio

doi:10.1016/j.freeradbiomed.2015.10.314

Cited by 2 publications

(2 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Sex hormones also contribute to the differences between sexes and are associated with incidence and progression of some diseases [ 20 – 22 ]. For example, estrogens are atheroprotective and vasoprotective and may exert potent antioxidant actions [ 21 ].…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Effects of Diacetyl Flavoring Exposure in Mice Metabolism

Jedlicka

Silva

Balbino

et al. 2018

BioMed Research International

View full text Add to dashboard Cite

Diacetyl is a flavoring that imparts a buttery flavor to foods, but the use or exposure to diacetyl has been related to some diseases. We investigated the effect of oral intake of diacetyl in male and female C57/Bl mice. We performed a target metabolomics assay using ultraperformance liquid chromatography paired with triple quadrupole mass spectrometry (UPLC-MS/MS) for the determination and quantification of plasmatic metabolites. We observed alterations in metabolites present in the urea and tricarboxylic acid (TCA) cycles. Peroxynitrite plasmatic levels were evaluated by a colorimetric method, final activity of superoxide dismutase (SOD) was evaluated by an enzymatic method, and mouse behavior was evaluated. Majority of the assay showed differences between control and treatment groups, as well as between genders. This may indicate the involvement of sex hormones in the regulation of a normal metabolic profile, and the implication of sex differences in metabolite disease response.

show abstract

Section: Resultsmentioning

confidence: 99%

“…Based on previous studies in our laboratory [ 20 ] and the study conducted by Colley et al [ 3 ], we chose a dosage of 300 mg/kg/day of diacetyl diluted in drinking water. Daily administration was chosen because diacetyl is a food flavoring widely used in the food industry, and it is considered safe by several agencies [ 21 – 24 ].…”

Section: Methodsmentioning

confidence: 99%