Alteration of iron (Fe), copper (Cu), zinc (Zn), and manganese (Mn) tissue levels and speciation in rats with desferioxamine-induced iron deficiency

Ajsuvakova, Olga P.; Skalnaya, Margarita G.; Michalke, Bernhard; Tinkov, Alexey A.; Serebryansky, Eugeny P.; Karganov, M. Yu.; Medvedeva, Yu. S.; Skalny, Anatoly V.

doi:10.1007/s10534-021-00318-9

Cited by 4 publications

(3 citation statements)

References 52 publications

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“…Using a single Agilent Bio SEC-5 column and a complex elution buffer containing 50 mM NH 4 Ac, 11 mM Tris-HAc, and ca. 2% methanol, Mn, Fe, Zn, and Cu speciation in Fe-deficient rats was examined [120]. Fe deficiency was induced with a desferrioxamine injection.…”

Section: Human Plasma Bovine Serum Bovine Albuminmentioning

confidence: 99%

Recent Advances in Metalloproteomics

Coverdale,

Polepalli,

Arruda

et al. 2024

Biomolecules

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Interactions between proteins and metal ions and their complexes are important in many areas of the life sciences, including physiology, medicine, and toxicology. Despite the involvement of essential elements in all major processes necessary for sustaining life, metalloproteomes remain ill-defined. This is not only owing to the complexity of metalloproteomes, but also to the non-covalent character of the complexes that most essential metals form, which complicates analysis. Similar issues may also be encountered for some toxic metals. The review discusses recently developed approaches and current challenges for the study of interactions involving entire (sub-)proteomes with such labile metal ions. In the second part, transition metals from the fourth and fifth periods are examined, most of which are xenobiotic and also tend to form more stable and/or inert complexes. A large research area in this respect concerns metallodrug–protein interactions. Particular attention is paid to separation approaches, as these need to be adapted to the reactivity of the metal under consideration.

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Section: Human Plasma Bovine Serum Bovine Albuminmentioning

confidence: 99%

Recent Advances in Metalloproteomics

Coverdale,

Polepalli,

Arruda

et al. 2024

Biomolecules

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“…The iron contents in digestive products were determined as described by Ajsuvakova et al [26] with minor adjustments. Briefly, the digestive products were mixed with nitric acid (16 mol/L) at a ratio of 25: 1 (w: w), and reacted in a microwave digester for 30 min (Milestone, Lab Tech, Italy).…”

Section: Determination Of Iron Content In Digestive Productsmentioning

confidence: 99%

The efficiency and safety evaluation of hemoglobin hydrolysate as a non-heme iron fortifier

Xue¹,

Jiang²,

Zhang³

et al. 2024

Food Science and Human Wellness

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Hemoglobin hydrolysate is derived from the enzymatic degradation of hemoglobin. This work aimed to evaluate whether hemoglobin hydrolysate promotes the absorption of non-heme iron and the safety of absorbed iron in mice by analyzing the iron binding content, iron circulation, and liver homeostasis. We found that hemoglobin hydrolysate promoted the absorption of non-heme iron with high efficiency in duodenum by spontaneously binding non-heme iron during digestion, and increased hepatic iron content by up-regulating divalent metal transporter 1 (DMT1), zinc transporter 14 (ZIP14), but hepatic iron content only increased at 3 weeks. Duodenal iron entered the blood through ferroportin without restriction at 3 weeks, and excessive iron entered the liver and then affected the hepatocyte membranes permeability and lipid synthesis through oxidative stress. With the prolongation of dietary intervention, the up-regulated hepcidin acted on the ferroportin to restrict excess iron from entering the blood, and then the hepatic homeostasis recovered. In addition, hemoglobin hydrolysate enhanced the hepatic antioxidant capacity. Taken together, hemoglobin hydrolysate has a strong ability to promote the absorption of non-heme iron in vivo, and the absorbed iron is relatively safe due to the regulation of hepcidin.

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“…Fe(II) and Fe(III) were determined in clinical samples by capillary electrophoresis (CE) with ICP MS [37], and they were determined in beverages with the use of a short anion-exchange column [38]. Other techniques of separation have also been used with ICP MS as a detector, e.g., size exclusion chromatography (SEC), although these techniques are useful in protein determination [39].…”

Section: Introductionmentioning

confidence: 99%

A Fast and Efficient Procedure of Iron Species Determination Based on HPLC with a Short Column and Detection in High Resolution ICP OES

2023

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The optimization and application of a new hyphenated procedure for iron ionic speciation, i.e., high performance liquid chromatography (HPLC) with short cation–exchange column (50 mm × 4 mm) coupled to high resolution inductively coupled plasma optical emission spectrometry (ICP hrOES), is presented in this paper. Fe(III) and Fe(II) species were separated on the column with the mobile phase containing pyridine–2,6–dicarboxylic acid (PDCA). The total time of the analysis was approx. 5 min, with a significantly low eluent flow rate (0.5 mL min−1) compared to the literature. Additionally, a long cation-exchange column (250 mm × 4.0 mm) was used as reference. Depending on the total iron content in the sample, two plasma views were chosen, e.g., an attenuated axial (<2 g kg−1) and an attenuated radial. The standard addition method was performed for the method’s accuracy studies, and the applicability was presented on three types of samples: sediments, soils, and archaeological pottery. This study introduces a fast, efficient, and green method for leachable iron speciation in both geological and pottery samples.

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Alteration of iron (Fe), copper (Cu), zinc (Zn), and manganese (Mn) tissue levels and speciation in rats with desferioxamine-induced iron deficiency

Cited by 4 publications

References 52 publications

Recent Advances in Metalloproteomics

Recent Advances in Metalloproteomics

The efficiency and safety evaluation of hemoglobin hydrolysate as a non-heme iron fortifier

A Fast and Efficient Procedure of Iron Species Determination Based on HPLC with a Short Column and Detection in High Resolution ICP OES

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