The efficiency and safety evaluation of hemoglobin hydrolysate as a non-heme iron fortifier

Xue, Dejiang; Jiang, Shuai; Zhang, Miao; Shan, Kai; Lametsch, René; Li, Chunbao

doi:10.26599/fshw.2022.9250086

Cited by 3 publications

(9 citation statements)

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“…It has been reported that hemoglobin hydrolysate diet and heme diet significantly raised the iron contents in duodenum. [ 43 ] The increase in iron content significantly reduced the L. reuteri content in duodenal contents. [ 44 ] In this study, the impact of completely digested myoglobin on L. reuteri may be attributed to hemin and peptides.…”

Section: Discussionmentioning

confidence: 99%

Dietary Hemin Remodels Gut Microbiota and Mediates Tissue Inflammation and Injury in the Small Intestine

Li,

Ke,

Jiang

et al. 2024

Molecular Nutrition Food Res

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ScopeEpidemiological studies have linked excessive red and processed meat intake to gut disorders. Under laboratory conditions, high heme content is considered the primary health risk factor for red meat. However, heme in meat is present in myoglobin, which is an indigestible protein, suggesting the different functions between myoglobin and heme. This study aims to explore how dietary myoglobin and heme affect gut health and microbiota differently.Methods and resultsHistological and biochemical assessments as well as 16S rRNA sequencing are performed. Moderate myoglobin intake (equivalent to the recommended intake of 150 g meat per day for human) has beneficial effects on the duodenal barrier. However, a too high myoglobin diet (equivalent to intake of 3000 g meat per day for human) triggers duodenum injury and alters the microbial community. The hemin diet destroys intestinal tissue and ileal microbiota more significantly. The in vitro experiments further confirm that free heme exhibits high toxicity to beneficial gut bacteria while myoglobin promotes the growth and metabolism of Limosilactobacillus reuteri.ConclusionModerate intake of myoglobin or hemin is beneficial to intestinal health and microbiota, but too high amounts lead to tissue inflammation and injury in the small intestine by reshaping ileal microbiota.

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Section: Discussionmentioning

confidence: 99%

Dietary Hemin Remodels Gut Microbiota and Mediates Tissue Inflammation and Injury in the Small Intestine

Li,

Ke,

Jiang

et al. 2024

Molecular Nutrition Food Res

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“…Among them, the concentration of unsaturated iron binding capacity (UIBC) derived from our previous research. 15 A A )…”

Section: Preparation Of Peptide−iron Complexmentioning

confidence: 99%

“…When it was observed under the microscope that the adherent area of cells reached 80%, the complete medium was removed, and 1 mL of complete medium containing peptide−iron complex was added, and the cells were incubated at 37 °C for 24 h. Then, the supernatant of the cell culture was collected. The iron content in the supernatant was determined according to the method of Xue et al 15 with some modifications. Briefly, the supernatant was diluted with ultrapure water, and the iron content was quantified using an inductively coupled plasma mass spectrometer (NexION 2000, PerkinElmer, USA).…”

Section: Determination Of Iron Contentmentioning

confidence: 99%

“…Iron Binding Capacity of Synthetic Peptides. The iron binding capacity of synthetic peptide was analyzed according to the method of Xue et al 15 with minor modifications. In brief, ferrous sulfate was added to the synthetic peptide solution at a ratio of 1:50 (v/v) and stood at 37 °C for 1 h. The ferrozine was added to the mixture as a chromogenic agent.…”

Section: Prediction Of Digestion Products Of Hemoglobin Hydrolysatementioning

confidence: 99%

“…16 Bioactive peptides derived from hemoglobin enhanced iron absorption in mice by spontaneously binding iron with the absorption efficiency equivalent to heme iron. 15 However, what peptides in hemoglobin hydrolyzate play a key role in promoting iron absorption and their underlying mechanism have not yet been elucidated.…”

Section: Introductionmentioning

confidence: 99%

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Hemoglobin Hydrolyzate Promotes Iron Absorption in the Small Intestine through Iron Binding Peptides

Xue,

Jiang,

et al. 2024

J. Agric. Food Chem.

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Hemoglobin is an excellent source of iron supplements, and its hydrolyzate spontaneously binds iron during digestion and promotes iron absorption in vivo. However, the underlying mechanisms of what peptides bind and how they bind iron ions remain unclear. This study prepared the porcine hemoglobin hydrolyzate through enzymatic hydrolysis and acid treatment and investigated the mechanisms of hemoglobin hydrolyzate on iron absorption through the determination of iron levels in dietary intervention mice, iron binding site analyses, peptide digestion analyses, molecular simulation docking, and INT407 cell validation. The results showed that ingestion of the hemoglobin hydrolyzate diets increased iron levels in the blood of mice, accompanied by the upregulation of duodenal iron circulation-related genes such as ferritin, PCBP1, and HP. Carboxyl, imidazole groups, and aromatic amino acid residues were iron binding sites of hemoglobin hydrolyzate during digestion. VDEVGGEA and VDEVGGE were found to involve the spontaneous and efficient binding of hemoglobin hydrolyzate to iron ions in the intestinal cavity. In particular, the DEVGGE peptide was the typical sequence for hemoglobin hydrolytic peptides to exert iron binding activity.

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The Role of Hemoglobin in Maintaining Health: A Literature Review

Agesti,

Damrah,

Rasyid

et al. 2024

j. info kesehat.

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The role of hemoglobin in maintaining health is still not fully understood Thoroughly. Therefore, this literature review aims to uncover comprehensively the role of hemoglobin in maintaining health. Objective: The purpose of this literature review is to investigate the role of hemoglobin in maintaining health by collecting and analyzing current findings in the scientific literature. The methodology used is a systematic search through academic databases such as PubMed, Google Scholar, and Scopus using relevant keywords such as "hemoglobin", "health maintenance", and "literature review". Articles that meet the inclusion criteria are selected for analysis. The samples in this review literature are scientific articles published within a certain time frame and focus on the role of hemoglobin in maintaining health. The population studied includes humans of various age groups and health conditions. The results of the analysis stated that hemoglobin plays an important role in maintaining oxygen balance in the body, supporting the function of vital organs, and preventing diseases related to hemoglobin disorders. Factors such as nutrition, environment, and health conditions can affect a person's hemoglobin levels. In conclusion, this literature review confirms that a deep understanding of hemoglobin's role in maintaining health is important for the development of prevention and management strategies for diseases associated with hemoglobin disorders. Thus, further research in this area is needed to improve general well-being.

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The efficiency and safety evaluation of hemoglobin hydrolysate as a non-heme iron fortifier

Cited by 3 publications

References 55 publications

Dietary Hemin Remodels Gut Microbiota and Mediates Tissue Inflammation and Injury in the Small Intestine

Dietary Hemin Remodels Gut Microbiota and Mediates Tissue Inflammation and Injury in the Small Intestine

Hemoglobin Hydrolyzate Promotes Iron Absorption in the Small Intestine through Iron Binding Peptides

The Role of Hemoglobin in Maintaining Health: A Literature Review

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