Alteration of chloroplast pigments by chromatography with siliceous adsorbents

Strain, Harold H.; Sherma, Joseph; Grandolfo, M.

doi:10.1021/ac60252a001

Cited by 53 publications

(9 citation statements)

References 34 publications

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“…/3-Carotene, for example, has recently been found to undergo significant hydroxylation and oxidation in the presence of Micro-Cel C to form isocryptoxanthin and other products (Rodriguez et al, 1976). An earlier report had linked Micro-Cel C and other siliceous adsorbents with the isomerization of carotenoid epoxides (Strain et al, 1967), and isomerization of carotenoids has also been attributed to adsorbtive alumina (Zechmeister, 1948;Zechmesiter and Sandoval, 1945;Tsukida and Zechmeister, 1958). It was also noted that fucoxanthin was quickly isomerized to isofucoxanthin when exposed to basic alumina .…”

mentioning

confidence: 98%

Transformation of 13,14- and 11,12-monoepoxycanthaxanthin on magnesia

Cyronak¹,

Osianu²,

Britton³

et al. 1978

J. Agric. Food Chem.

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mentioning

confidence: 98%

Transformation of 13,14- and 11,12-monoepoxycanthaxanthin on magnesia

Cyronak¹,

Osianu²,

Britton³

et al. 1978

J. Agric. Food Chem.

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“…This behavior is consistent with the presence of two 5,6-epoxy groups as in violaxanthin (Davies 1965). A comparison of the pigment in ethanol with 0.5-1y0 glacial acetic acid resulted in the appearance of absorption maxima at nm 381,400, 423, 447 within 1.5-2 h. These new absorptions reflect the conversion of violaxanthin into a mixture of luteoxanthin and auroxanthin (Strain et al 1967).…”

Section: Resultsmentioning

confidence: 95%

“…The gradual 5:5:1:3 v/v (Fischer and Dorfel 1955). The uronides, conversion of violaxanthin to luteoxanthin and auroxan-located by spraying a guide strip with aniline trichlorothin was recorded spectrally in samples made 0.5% with acetic acid, were eluted and subjected to paper electroglacial acetic acid (Strain et al 1967). phoresis in 0.01 M borate containing 0.005 M CaClz (Haug and Larsen 1961).…”

Section: Pigment Analysismentioning

confidence: 99%

Pigments, polysaccharides, and photosynthetic products of Phaeosaccion collinsii

Leigh¹,

Chen²,

McLachlan³

1971

Can. J. Bot.

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1971. Pigments, polysaccharides, and photosynthetic products of Phaeosaccion colli~rsii. Can. J. Bot. 49: 1067-1074. Zoospores of Phaeosaccion collinsii averaged 70 p3 and contained total N 2.24 pg, DNA 0.19, and chlorophyll a 0.62 pg per spore. In addition, chl c, "cis"-8-carotene, fucoxanthin, and violaxanthin were principal pigments and a minor unidentified carotene was present.Photosynthetic rates of zoospores were 0.37 X 10-l2g C per spore per hour, or 0.59 mg C per mg chl a per hour at 5". The principal products of photosynthesis were mamitol and a water-soluble glucan.Carbohydrates in the thalli were fractionated and evidence is presented for the presence of a laminarinlike glucan, mamitol, tnyo-and scyllo-inositols. Alginate was absent.The relationship of P. colli~uii to other algal groups is discussed and it is concluded that its affinities lie with the Chrysophyceae.

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“…The chromatography of carotenoid pigments on silica gel has its risks. As studied by Strain et al (1967), isomerization may occur, particularly that of violaxanthin and neoxanthin, to provide bands of cis isomers not normally encountered. Furthermore, the degradation of violaxanthin to auroxanthin is so common that the two pigments commonly occur together.…”

Section: Discussionmentioning

confidence: 99%