“…The extraction method utilised up to 20-fold less raw material and less than half the solvent of the commonly applied modified HarvestPlus approach (Champagne et al, 2010;Ferede et al, 2010;Ukom et al, 2014), whilst attaining profiles which matched some of those previously recorded, with many xanthophyll esters, e.g., D. alata (Champagne et al, 2010), D. bulbifera (Martin et al, 1974) and D. cayennensis (Martin & Ruberte, 1975) and stereoisomers present. The scaled-down extraction allowed increased throughput with easier sample handling and, consequently, the relatively long run-time of the HPLC was the limiting factor for the speed of screening.…”