Alpha-Amylase Inhibitory Activity of Extract Combination of Morinda citrifolia L. Fruit, Trigonella foenum-graecum and Nigella sativa L. Seeds Using In vitro and In Vivo Assay

Abstract

Maintaining glycemic control in Diabetes Mellitus (DM) is necessary to prevent many health complications, mortality, and morbidity. 1 There are various pharmacological approaches used in DM therapy such as delay the digestion and absorption of carbohydrates in the gastrointestinal system by inhibiting alpha-amylase and alphaglucosidase activity. 2 Alpha-amylase will converts polysaccharides in the mouth and intestines into the disaccharide molecule, then the reaction will be continued by alpha-glucosidase to produce glucose units that will be absorbed into the body. 3 This inhibition can lessen postprandial hyperglycemia and prevent diabetic complications. 4 There are many studies about the alpha-amylase inhibitory activity of plants and their bioactive compounds in vitro, but few studies confirm these findings in rodents and very few in humans. 5 In vivo and in vitro studies may not always be correlated, but in vitro data can be used to determine the activity of compounds before use in in vivo studies to determine physiological and pharmacological effects in animals. 6 Based on the previous study, the ethanol extract of Fenugreek seeds (FS), Morinda Fruit (MS), and Black Cumin Seeds (BCS) were reported to have antidiabetic activity, 7-9 but there were no reports regarding the inhibitory activity of alpha-amylase from the combined

Hypoglycemic Activity of Ethyl Acetate Fraction Combination of Morinda Fruit and Cinnamon Bark Using Glucose-Induced in Mice

“…Based on the table above the dosage group 5 has a decrease that is almost the same as the positive control that is glibenclamide it is in line with research conducted by Jin (2007) [18] that noni fruit as hypoglycemic that can lower blood glucose levels in mice. The content of flavonoid noni fruit has therapeutic properties that can affect the digestion of carbohydrates, fat deposition and insulin release [19]. Flavonoids also have an inhibitory effect on the enzyme α-glucosidase through hydroxylation bonds and β-ring substitution.…”