Aloe vera increases mRNA expression of antioxidant enzymes in cryopreserved bovine ovarian tissue and promotes follicular growth and survival after in vitro culture

Costa, Francisco das Chagas; Vasconcelos, Erlândia Márcia; Azevedo, Venância Antônia Nunes; Paulino, Laís R F M; Soares, Mônica Dias; Silva, José Roberto Viana; Silva, Anderson Weiny Barbalho; Souza, Ana Liza Paz

doi:10.1016/j.cryobiol.2021.07.005

Cited by 11 publications

(7 citation statements)

References 37 publications

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“…During cyropreservation, mitochondrial dysfunction can lead to an imbalance in oxidative status, resulting in changes in tissue structure and morphology, ultimately leading to follicular loss. Our data indicate that follicle morphology and ovarian matrix collagen fiber content both decrease after cryopreservation compared to fresh tissue, consistent with previous studies 21,42 . The thawing protocol used in this study had beneficial effects on follicular morphology.…”

Section: Discussionsupporting

confidence: 91%

“…Our data indicate that follicle morphology and ovarian matrix collagen fiber content both decrease after cryopreservation compared to fresh tissue, consistent with previous studies. 21,42 The thawing protocol used in this study had beneficial effects on follicular morphology. However, the findings of the current study do not support the previous research.…”

Section: Discussionmentioning

confidence: 98%

“…Despite the continuous development of OT cryopreservation techniques, it has been observed that tissue damage remains a constant and inevitable factor due to the heterogeneous cellular composition of ovaries 18 . Cryopreserved tissues are sensitive to oxidative stress and apoptosis, resulting in follicular apoptosis and stromal cell death 19–21 . This excessive reactive oxygen species (ROS) production has negative effects on reproduction, including reduced development of embryos and the emergence of progeny diseases, despite the fact that ROS are naturally produced during normal intracellular physiology 22–25 .…”

Section: Introductionmentioning

confidence: 99%

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Static magnetic field assisted thawing improves cryopreservation of mouse whole ovaries

Zhang,

Chi,

Cheng

et al. 2023

Bioengineering & Transla Med

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Ovarian tissue cryopreservation is considered to be the only means to preserve fertility for prepubertal girls and women whose cancer treatment cannot be postponed. However, ovarian tissues are inevitably damaged by oxidative stress during cryopreservation, which threatens follicle survival and development, and thus affects female fertility. Therefore, reducing tissue oxidative stress injury is one of the major challenges to achieving efficient cryopreservation of ovarian tissues, especially for whole ovaries. Here, we proposed a new method to improve the antioxidant capacity of whole ovaries during cryopreservation, static magnetic field assisted thawing. The results demonstrated that the antioxidant capacity of the ovarian tissue was significantly improved by static magnetic field treatment. In addition, ovarian tissue allograft transplantation was carried out, which successfully achieved vascular regeneration and maintained follicular development. The findings of this study not only provide a new reference for the preservation of female fertility, but also is a major step forward in the cryopreservation of tissues and organs. It will have good application prospects in the field of assisted reproduction and cryo‐biomedicine.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

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Static magnetic field assisted thawing improves cryopreservation of mouse whole ovaries

Zhang,

Chi,

Cheng

et al. 2023

Bioengineering & Transla Med

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“…According to the prediction of the World Health Organization (WHO), 80% of the global population utilizes TM as a complementary or alternative medicine [ 32 ]. To date, many herbal extracts and specific natural compounds have shown anti-inflammation and antioxidant effects, including those in A. vera [ 33 , 34 ]. In the present study, we demonstrated that aloesone, a major metabolic compound of A. vera, has multiple protective effects against oxidative stress, inflammation, M1 polarization, and apoptosis.…”

Section: Discussionmentioning

confidence: 99%

Multiple Beneficial Effects of Aloesone from Aloe vera on LPS-Induced RAW264.7 Cells, Including the Inhibition of Oxidative Stress, Inflammation, M1 Polarization, and Apoptosis

Wang

Xiong

et al. 2023

Molecules

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Aloesone is a major metabolic compound in Aloe vera, which has been widely used as a food source and therapeutic agent in several countries. Our recent study demonstrated that aloesone has anti-epileptic effects on glutamate-induced neuronal injury by suppressing the production of reactive oxygen species (ROS). Unless ROS are naturally neutralized by the endogenous antioxidant system, they lead to the activation of inflammation, polarization, and apoptosis. This study aimed to identify the multiple beneficial effects of aloesone and explore its molecular mechanism in macrophages. Hence, the murine macrophage cell line RAW264.7 was pretreated with aloesone and then exposed to lipopolysaccharides (LPS). The results demonstrated that aloesone, within a dosage range of 0.1–100 µM, dramatically decreased the LPS-induced elevation of ROS production, reduced nitric oxide (NO) release, inhibited the M1 polarization of RAW264.7 cells, and prevented cells from entering the LPS-induced early and late phases of apoptosis in a dose-dependent manner. Simultaneously, aloesone significantly decreased the mRNA expression of inflammation-related genes (iNOS, IL-1ꞵ, TNF-α) and increased the expression of antioxidant enzymes (Gpx-1 and SOD-1). The core genes HSP90AA1, Stat3, Mapk1, mTOR, Fyn, Ptk2b, and Lck were closely related to these beneficial effects of aloesone. Furthermore, immunofluorescence staining and flow cytometry data confirmed that aloesone significantly repressed the activation of mTOR, p-mTOR, and HIF-1α induced by LPS and inhibited the protein expression of TLR4, which is the target of LPS. In conclusion, aloesone demonstrated multiple protective effects against LPS-induced oxidative stress, inflammation, M1 polarization, and apoptosis in macrophages, suggesting its potential as a prodrug.

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“…Some clinical and experimental evidence suggested the usefulness of Aloe vera in wound care and wound healing for skin moisturization, promoting wound healing, thermal skin injury, and inflammatory skin pathologies [17,[23][24][25][26][27][28]. Therefore, in vitro/in vivo trials using Aloe vera gel, as a scaffold or support matrix for tissue engineering, have received considerable attention either alone or in combination with conventional drugs or natural extracts [5,11,[29][30][31][32][33][34][35][36][37][38][39][40][41][42][43][44][45]. For instance, the use of fresh Aloe vera gel for 10 days to treat breast soreness and irritation in lactating women produced significant wound healing [43].…”

Section: Introductionmentioning

confidence: 99%

Characterization and Topical Study of Aloe Vera Hydrogel on Wound-Healing Process

et al. 2021

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Wound healing is fundamental to restore the tissue integrity. A topical study of the influence of Aloe vera hydrogel, formulated with 1,2-propanediol (propanediol) and triethanolamine (TEA), on the skin wound-healing process was investigated in female Wistar rats. FTIR spectroscopy confirms the presence of carboxylic acid and methyl ester carboxylate groups related with important compounds that confer the hydrogel a good interaction with proteins and growth factors. SEM images show a microstructure and micro-roughness that promote a good adhesion to the wound. Therefore, the swelling kinetics and the contact angle response contribute to the understanding of the in vivo results of the animal test. The results indicated that the Aloe vera hydrogel, prepared with propanediol and TEA, together with its superficial characteristics, improve its rapid penetration without drying out the treated tissue. This produced a positive influence on inflammation, angiogenesis, and wound contraction, reducing 29% the total healing time, reaching the total closure of the wound in 15 days.

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Aloe vera increases mRNA expression of antioxidant enzymes in cryopreserved bovine ovarian tissue and promotes follicular growth and survival after in vitro culture

Cited by 11 publications

References 37 publications

Static magnetic field assisted thawing improves cryopreservation of mouse whole ovaries

Static magnetic field assisted thawing improves cryopreservation of mouse whole ovaries

Multiple Beneficial Effects of Aloesone from Aloe vera on LPS-Induced RAW264.7 Cells, Including the Inhibition of Oxidative Stress, Inflammation, M1 Polarization, and Apoptosis

Characterization and Topical Study of Aloe Vera Hydrogel on Wound-Healing Process

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