Alloscardovia macacae sp. nov., isolated from the milk of a macaque (Macaca mulatta), emended description of the genus Alloscardovia and proposal of Alloscardovia criceti comb. nov.

Killer, Jiří; Ročková, Šárka; Vlková, Eva; Rada, V.; Havlík, Jaroslav; Kopečný, J.; Bunešová, Věra; Benada, Oldřich; Kofroňová, Olga; Pechar, Radko; Profousová, Ilona

doi:10.1099/ijs.0.051326-0

Cited by 27 publications

(9 citation statements)

References 33 publications

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“…The sequences of the 16S rRNA marker genes were obtained from the RAST annotations of UMA81211 and UMA81212 and showed 98.67% and 98.27% similarity to Alloscardovia macacae M8, respectively (3, 4). The next highest similarity scores were for Alloscardovia criceti DSM17774 (UMA81211, 96.54%; UMA81212, 96.31%) and Alloscardovia omnicolens DSM21503 (UMA81211, 96.54%; UMA81212, 95.93%) (3, 5).…”

Section: Genome Announcementmentioning

confidence: 99%

“…The next highest similarity scores were for Alloscardovia criceti DSM17774 (UMA81211, 96.54%; UMA81212, 96.31%) and Alloscardovia omnicolens DSM21503 (UMA81211, 96.54%; UMA81212, 95.93%) (3, 5). The full 16S rRNA sequences of UMA81211, UMA81212, and representative members of the family Bifidobacteriaceae were used to generate a neighbor-joining phylogenetic tree with bootstrapping using MEGA7 and the EZBioCloud database (4, 6).…”

Section: Genome Announcementmentioning

confidence: 99%

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Draft Genome Sequences of Alloscardovia macacae UMA81211 and UMA81212, Isolated from the Feces of a Rhesus Macaque (Macaca mulatta)

Albert

Sela

2017

Genome Announc

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Section: Genome Announcementmentioning

confidence: 99%

Section: Genome Announcementmentioning

confidence: 99%

Draft Genome Sequences of Alloscardovia macacae UMA81211 and UMA81212, Isolated from the Feces of a Rhesus Macaque (Macaca mulatta)

Albert

Sela

2017

Genome Announc

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“…Growth of strain DPVI-TET3 T at different temperatures and pH was determined as described previously (Killer et al, 2013b). Sensitivity to the presence of atmospheric oxygen was investigated by means of cultivation on TPY agar (Killer et al, 2010).…”

Section: J Killer and Others 2934mentioning

confidence: 99%

“…This methodological approach has been used successfully before in a similar case (Killer et al, 2013b). Phylogenetic markers useful in the taxonomy of the family Bifidobacteriaceae, partial sequences of the hsp60, fusA, xfp, tuf, rpoC, rplB, gyrB and dnaK genes, were considered.…”

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confidence: 99%

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Pseudoscardovia radai sp. nov., a representative of the family Bifidobacteriaceae isolated from the digestive tract of a wild pig (Sus scrofa scrofa)

Killer

Havlík

Bunešová

et al. 2014

International Journal of Systematic and Evolutionary Microbiology

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Pseudoscardovia radai sp. nov., a representative of the family Bifidobacteriaceae isolated from the digestive tract of a wild pig (Sus scrofa scrofa) The presence of bifidobacteria and representatives of the new genus Pseudoscardovia within the family Bifidobacteriaceae in the digestive tract of wild pigs was reported recently. Results based on comparative 16S rRNA gene sequence analysis of a new fructose-6-phosphate phosphoketolase-positive bacterial isolate, strain DPVI-TET3 T , originating from the small intestine of a wild pig revealed a relationship to Pseudoscardovia suis DPTE4 T (96.8 % sequence similarity). Phylogenetic and comparative analyses based on 16S rRNA, hsp60, xfp, fusA, tuf and rpoC partial gene sequences confirmed the relationship of the novel bacterial strain to P. suis DPTE4 T in comparison with other bifidobacterial species occurring in the digestive tract of domestic and wild pigs. Differences in utilization of various substrates, production of enzymes, cell morphology, peptidoglycan structure and profiles of cellular fatty acids and polar lipids between strain DPVI-TET3 T and P. suis DPTE4 T allow the establishment of a novel species, for which the name Pseudoscardovia radai sp. nov. is proposed. The type strain is strain DPVI-TET3 T (5CCM 7943 T 5DSM 24742 T ).Bacterial diversity in the gastrointestinal tract of wild mammals is still not adequately explored (Nelson et al., 2003; Xu et al., 2010) and is influenced by many factors such as food composition, age, health, individual variation, environment, geographical distribution and physiological state of the host (Thompson-Chagoyán et al., 2007; Ochman et al., 2010). Isolation and characterization of representatives of novel bacterial taxa within these specific ecological niches is inter alia important from the viewpoint of usage of their potentially beneficial properties in humans and economically important animals (Walter, 2008;Trebichavsky et al., 2009;Rossi et al., 2011). The most studied groups of bacteria by the scientific community with the potential for positive effects on host health are the bifidobacteria and lactobacilli (Tejero-Sariñena et al., 2013;Sikorska & Smoragiewicz, 2013;Vlasova et al., 2013).Species of bifidobacteria from animals are found in the digestive tracts of primates (Endo et al., 2012;Tsuchida et al., 2014), domesticated pigs, rabbits, cattle, poultry, honeybees (Biavati & Mattarelli, 2012) and bumblebees (Killer et al., 2009(Killer et al., , 2011. In recent years, some fructose-6-phosphate phosphoketolase-positive bacteria have been classified into new scardovial genera phylogenetically different from members of the genus Bifidobacterium (Jian & Dong, 2002;Killer et al., 2010Killer et al., , 2013b.The presence of a differential bifidobacterial population in the digestive tract of wild pigs has been reported recently. A representative of a new genus, Pseudoscardovia, was described in that study (Killer et al., 2013a). This study comprises the detailed characterization of a novel bacterial species most clo...

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Gene encoding the CTP synthetase as an appropriate molecular tool for identification and phylogenetic study of the family Bifidobacteriaceae

et al. 2018

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An alternative molecular marker with respect to the 16S rRNA gene demonstrating better identification and phylogenetic parameters has not been designed for the whole Bifidobacteriaceae family, which includes the genus Bifidobacterium and scardovial genera. Therefore, the aim of the study was to find such a gene in available genomic sequences, suggest appropriate means and conditions for asmplification and sequencing of the desired region of the selected gene in various strains of the bacterial family and verify the importance in classification and phylogeny. Specific primers flanking the variable region (~800 pb) within the pyrG gene encoding the CTP synthetase were designed by means of gene sequences retrieved from the genomes of strains belonging to the family Bifidobacteriaceae. The functionality and specificity of the primers were subsequently tested on the wild (7) and type strains of bifidobacteria (36) and scardovia (7). Comparative and phylogenetic studies based on obtained sequences revealed actual significance in classification and phylogeny of the Bifidobacteriaceae family. Gene statistics (percentages of mean sequence similarities and identical sites, mean number of nucleotide differences, P‐ and K‐distances) and phylogenetic analyses (congruence between tree topologies, percentages of bootstrap values >50 and 70%) indicate that the pyrG gene represents an alternative identification and phylogenetic marker exhibiting higher discriminatory power among strains, (sub)species, and genera than the 16S rRNA gene. Sequences of the particular gene fragment, simply achieved through specific primers, enable more precisely to classify and evaluate phylogeny of the family Bifidobacteriaceae including, with some exceptions, health‐promoting probiotic bacteria.

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Alloscardovia macacae sp. nov., isolated from the milk of a macaque (Macaca mulatta), emended description of the genus Alloscardovia and proposal of Alloscardovia criceti comb. nov.

Cited by 27 publications

References 33 publications

Draft Genome Sequences of Alloscardovia macacae UMA81211 and UMA81212, Isolated from the Feces of a Rhesus Macaque (Macaca mulatta)

Draft Genome Sequences of Alloscardovia macacae UMA81211 and UMA81212, Isolated from the Feces of a Rhesus Macaque (Macaca mulatta)

Pseudoscardovia radai sp. nov., a representative of the family Bifidobacteriaceae isolated from the digestive tract of a wild pig (Sus scrofa scrofa)

Gene encoding the CTP synthetase as an appropriate molecular tool for identification and phylogenetic study of the family Bifidobacteriaceae

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