Allitridin inhibits human cytomegalovirus replication in vitro

Zhang, Ju; Wang, Hui; Xiang, Zhangmin; Shu, Sainan; Fang, Feng

doi:10.3892/mmr.2013.1328

Cited by 5 publications

(3 citation statements)

References 25 publications

(23 reference statements)

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“…In fact, we could not detect the mRNA encoding IE2 by qRT-PCR (data not shown). Finally, normalization of the expression data to that of GAPDH did not significantly alter the findings [14,23]. Our findings suggest that hsa-miR-200b-3p and -200c-3p could play relevant roles in regulating the initiation of HCMV active replication and/or latent-to-lytic switch by blocking the synthesis of major IE2 protein.…”

Section: Discussionmentioning

confidence: 57%

“…To exclude the effects of severity of necrosis on HCMV quantity [14,23], we measured the expression of the housekeeping protein glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in 100 ng/μl of gDNA through qPCR using TaqMan™ human GAPDH endogenous control (6FAM™/MGB probe, non-primer limited) according to the manufacturer’s instructions (Applied Biosystems/Thermo Fisher Scientific). qPCRs were run in a LightCycler ® 480 platform (Roche Diagnostics, Seoul, Korea).…”

Section: Methodsmentioning

confidence: 99%

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Expression of human miR-200b-3p and -200c-3p in cytomegalovirus-infected tissues

et al. 2018

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Human cytomegalovirus (HCMV) infection can cause inflammatory tissue-invasive end-organ diseases upon lytic replication. In humans, mature miR-200b-3p and -200c-3p suppress the synthesis of HCMV immediate early 2 (IE2) protein by binding to the 3′-UTR of the mRNA encoded by the unique long (UL) 122-123 region in human foreskin fibroblasts and pre-transplant peripheral blood mononuclear cells stimulated with HCMV. The present study aimed to quantitate the expression of Homo sapiens (hsa)-miR-200b-3p and 200c-3p in HCMV-infected tissues. We collected 240 HCMV-infected and 154 HCMV-non-infected, formalin-fixed, paraffin-embedded tissue samples of the gastrointestinal (GI) tract and bronchi/lungs. MiRNAs, HCMV, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were quantitated by quantitative reverse transcription-PCR (qRT-PCR) and quantitative PCR (qPCR) on the basis of standard curves generated using miRNA mimics, the HCMV strain from National Institute for Biological Standards and Control (NIBSC) 09/162, and GAPDH control. To avoid the effect of cell counts on the qRT-PCR and qPCR results, the data were normalized to GAPDH levels. HCMV-infected tissues had significantly lower levels of 200b-3p/GAPDH (3.03 ± 1.50 compared with 3.98 ± 1.08 log10 copies/μl, P<0.001) and 200c-3p/GAPDH (4.67 ± 1.84 compared with 6.35 ± 1.47 log10 copies/μl, P<0.001) than normal tissues. The values for 200b-3p/GAPDH (r = −0.51, P<0.001) and 200c-3p/GAPDH (r = −0.54, P<0.001) were significantly inversely correlated with HCMV load. Low tissue levels of 200b-3p and 200c-3p in humans are associated with cytopathic inflammation due to HCMV infection.

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Section: Discussionmentioning

confidence: 57%

Section: Methodsmentioning

confidence: 99%

Expression of human miR-200b-3p and -200c-3p in cytomegalovirus-infected tissues

et al. 2018

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“…Studies from the same group showed the beneficial effect of allitridin on liver pathology after CMV infection and reduction of viral load in affected organs (67,68). The effect of allitridin was attributed to the inhibition of viral gene transcription (69,70) and to decreased immune tolerance to the infection by reducing amplification of T-regulatory helper cells after CMV infection (71,72).…”

Section: Natural Sulfur-containing Compoundsmentioning

confidence: 97%

Hydrogen Sulfide: A Novel Player in Airway Development, Pathophysiology of Respiratory Diseases, and Antiviral Defenses

Bazhanov¹,

Ansar

Ivanciuc³

et al. 2017

Am J Respir Cell Mol Biol

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Hydrogen sulfide (HS) is a biologically relevant signaling molecule in mammals. Along with the volatile substances nitric oxide (NO) and carbon monoxide (CO), HS is defined as a gasotransmitter. It plays a physiological role in a variety of functions, including synaptic transmission, vascular tone, angiogenesis, inflammation, and cellular signaling. The generation of HS is catalyzed by cystathionine β-synthase (CBS), cystathionine γ-lyase (CSE), and 3-mercaptopyruvate sulfurtransferase (3-MST). The expression of CBS and CSE is tissue specific, with CBS being expressed predominantly in the brain, and CSE in peripheral tissues, including lungs. CSE expression and activity are developmentally regulated, and recent studies suggest that CSE plays an important role in lung alveolarization during fetal development. In the respiratory tract, endogenous HS has been shown to participate in the regulation of important functions such as airway tone, pulmonary circulation, cell proliferation or apoptosis, fibrosis, oxidative stress, and inflammation. In the past few years, changes in the generation of HS have been linked to the pathogenesis of a variety of acute and chronic inflammatory lung diseases, including asthma and chronic obstructive pulmonary disease. Recently, our laboratory made the critical discovery that cellular HS exerts broad-spectrum antiviral activity both in vitro and in vivo, in addition to independent antiinflammatory activity. These findings have important implications for the development of novel therapeutic strategies for viral respiratory infections, as well as other inflammatory lung diseases, especially in light of recent significant efforts to generate controlled-release HS donors for clinical therapeutic applications.

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Allium sativum

Lim¹

2014

Edible Medicinal and Non Medicinal Plants

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Allitridin inhibits human cytomegalovirus replication in vitro

Cited by 5 publications

References 25 publications

Expression of human miR-200b-3p and -200c-3p in cytomegalovirus-infected tissues

Expression of human miR-200b-3p and -200c-3p in cytomegalovirus-infected tissues

Hydrogen Sulfide: A Novel Player in Airway Development, Pathophysiology of Respiratory Diseases, and Antiviral Defenses

Allium sativum

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