Alleviation of Experimental Septic Shock in Mice by Acidic Polysaccharide Isolated from the Medicinal Mushroom Phellinus linteus

Kim, Gi‐Young; Roh, Su-In; Park, Soonkew; Ahn, Soon‐Cheol; Oh, Yang-Hyo; Lee, Jae-Dong; Park, Yeong‐Min

doi:10.1248/bpb.26.1418

Cited by 54 publications

(39 citation statements)

References 30 publications

(24 reference statements)

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“…Recently, polysaccharides isolated from Phellinus mushrooms have received special attention due to their potent pharmacological activities, including anti-tumor [2,4,5,10], immunostimulating [15], and anti-inflammatory properties [13,14]. Among these Phellinus mushrooms, PG used in this study has advantages over the other Phellinus mushrooms in that it has a very short growth period (3 months) compared to other Phellinus mushrooms (2-3 years) making it cheaper to produce.…”

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confidence: 99%

Promotion of Dermal Wound Healing by Polysaccharides Isolated from Phellinus gilvus in rats

Bae

Jang

Park

et al. 2005

The Journal of Veterinary Medical Science

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ABSTRACT. The effects of topical administration of polysaccharides isolated from fungus, Phellinus gilvus (PG) on the healing of rat dermal wounds were assessed. In 10 Sprague-Dawley (SD) rats, six 6 mm diameter defects were made with a punch biopsy appliance. After 24 hr, test substances were applied to the defects twice a day: 0.025, 0.25, and 2.5% polysaccharides from PG (PG0.025, 0.25, and 2.5 groups), Madecassol ® ointment (MC group), aqueous gel (AG group) and no treatment (control group). Six days postoperatively, the contraction and reepithelialization of the wound surface were assessed. Wound diameter was significantly reduced in all PG groups (P<0.05). Complete epithelialization and macrophages were noted in the PG0.25 group, as compared to the control group. We conclude that polysaccharides isolated from PG have significant dermal wound healing effects, and this investigation suggests the potential clinical application of PG as a wound healing agent.

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confidence: 99%

Promotion of Dermal Wound Healing by Polysaccharides Isolated from Phellinus gilvus in rats

Bae

Jang

Park

et al. 2005

The Journal of Veterinary Medical Science

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“…Reverse transcription: A 1 µg portion of total RNA was subjected to first-strand cDNA synthesis in a 20 µl reaction mixture containing moloney murine leukemia virus reverse transcriptase (10 U), dNTP mixture (2.5 mM of each dNTP), oligo(dT) [12][13][14][15][16][17][18] primers (10 µM), and reaction buffer as supplied with the enzyme (50 mM Tris-HCl (pH 8.3), 50 mM KCl, 10 mM MgCl 2 , 0.5 mM spermidine, and 10 mM dithiothreitol). The samples were incubated in a TOUCHgene DNA thermal cycler (Techne (Cambridge) Limited, U.K.) at 42°C for 60 min followed by an enzyme denaturation step at 94°C for 2 min.…”

Section: Preparation Of Materialsmentioning

confidence: 99%

“…Polysaccharides isolated from mushrooms such as Phellinus gilvus (PG) and Phellinus linteus (PL) used in our study are well-known medicinal sources due to their anti-tumor [11], immunostimulating [19], and anti-inflammatory activities [15,17,18]. Their anti-inflammatory activities suggest that these natural products may be beneficial in the treatment of intraperitoneal adhesion related to inflammation.…”

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confidence: 99%

The Effect of Polysaccharides and Carboxymethylcellulose Combination to Prevent Intraperitoneal Adhesion and Abscess Formation in a Rat Peritonitis Model

Bae

Jin

Jang

2004

The Journal of Veterinary Medical Science

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ABSTRACT. Polysaccharides isolated from fungi, Phellinus spp. is well-known material with anti-tumor and anti-inflammatory properties. We have assessed the adhesion-and abscess-reducing capacity of carboxymethylcellulose (CMC) and polysaccharides from Phellinus spp. combination in a rat peritonitis model. In 72 Sprague-Dawley rats, experimental peritonitis was induced by means of the ce cal ligation and puncture model (CLP). After 24 hr, the abdomen was reopened and the ligated cecum was resected. Peritoneal fluid samples were taken for microbiological examination. Rats were randomly assigned to 6 groups: ringer lactate solution (RL group), polysaccharides from Phellinus gilvus (PG group) and Phellinus linteus (PL group), carboxymethylcellulose (CMC group), and their combinations (PG+CMC and PL+CMC groups). Adhesions and abscesses were noted at day 7 after CLP. RT-PCR assay for urokinase-type plasminogen activator (uPA), its cellular receptor (uPAR), and tumor necrosis factor (TNF)-α was performed to assess the cecal tissue. Microbiological examination showed polymicrobial bacterial peritonitis. Adhesion formation was significantly reduced in PG+CMC and PL+CMC groups (P<0.05). The incidence of abscesses was reduced in all treated groups except the RL group (P<0.05). uPA, uPAR, and TNF-α mRNA were highly expressed in the PG+CMC and PL+CMC groups, as compared to the RL group. We concluded that the combination of polysaccharides and CMC had significant adhesion-and abscess-reducing effects compared with their single treatment and the effects may act by modifying the fibrinolytic capacity of uPA, uPAR and TNF-α produced from activated macrophages in a rat peritonitis model.

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“…Several in vitro and in vivo mechanisms have been related to the anti-inflammatory activity of polysaccharides and extracts rich in fungal polysaccharides. Among these mechanisms are cyclooxygenase inhibition (Hong et al, 2004), inhibition, or decrease in cytokine levels (Kim et al, 2003) and inhibition of inflammatory cell recruitment (Dore et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Polysaccharides from the fungus Scleroderma nitidum with anti-inflammatory potential modulate cytokine levels and the expression of Nuclear Factor kB

Nascimento¹,

Magalhães²,

Pinheiro³

et al. 2012

Rev. bras. farmacogn.

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Several pharmacological properties are attributed to polysaccharides and glucans derived from fungi such as tumor, anti-inflammatory, and immunomodulatory activity. In this work, the anti-inflammatory potential of polysaccharides from the fungus Scleroderma nitidum and their possible action mechanism were studied. The effect of these polymers on the inflammatory process was tested using the carrageenan and histamine-induced paw edema model and the sodium thioglycolate and zymosan-induced model. The polysaccharides from S. nitidum were effective in reducing edema (73% at 50 mg/kg) and cell infiltrate (37% at 10 mg/kg) in both inflammation models tested. Nitric oxide, a mediator in the inflammatory process, showed a reduction of around 26% at 10 mg/kg of body weight. Analysis of pro-and anti-inflammatory cytokines showed that in the groups treated with polysaccharides from S. nitidum there was an increase in cytokines such as IL-1ra, IL-10, and MIP-1β concomitant with the decrease in INF-γ (75%) and IL-2 (22%). We observed the influence of polysaccharides on the modulation of the expression of nuclear factor κB. This compound reduced the expression of NF-κB by up to 64%. The results obtained suggest that NF-κB modulation an mechanisms that explain the antiinflammatory effect of polysaccharides from the fungus S. nitidum.

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Alleviation of Experimental Septic Shock in Mice by Acidic Polysaccharide Isolated from the Medicinal Mushroom Phellinus linteus

Abstract: This study reports that acidic polysaccharide (PL) isolated from

Cited by 54 publications

References 30 publications

Promotion of Dermal Wound Healing by Polysaccharides Isolated from Phellinus gilvus in rats

Promotion of Dermal Wound Healing by Polysaccharides Isolated from Phellinus gilvus in rats

The Effect of Polysaccharides and Carboxymethylcellulose Combination to Prevent Intraperitoneal Adhesion and Abscess Formation in a Rat Peritonitis Model

Polysaccharides from the fungus Scleroderma nitidum with anti-inflammatory potential modulate cytokine levels and the expression of Nuclear Factor kB

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