Allergic phenotypes in adult patients with atopic dermatitis, determined with the ISAC test (ImmunoCAP ISAC)

Abstract

IntroductionAtopic dermatitis (AD) is a chronic inflammatory dermatosis, often with a concomitant allergy. The ImmunoCAP ISAC (Immuno Solid-Phase Allergen Chip) test is a novel method to determine the allergenic phenotype in a given patient.AimIn this study, we used the ImmunoCAP ISAC test to analyze allergic phenotypes in adult patients with AD.Material and methodsThe study included 19 adult patients with AD. The severity of AD was assessed using SCORAD index. Serum concentrations of total IgE were determined by means fluoro-enzyme immunoassay (FEIA). The levels of asIgE were measured with the ImmunoCAP ISAC kits.ResultsPositive results of the ISAC test were documented in 84.2% of the study subjects. All patients synthesized asIgE against species-specific respiratory allergens; major components of animal allergens (57.87%), tree pollen allergens (47.3%), grass pollen allergens (42.1%), dust mite allergens (26.3%) and major allergen of mugwort (26.3%). 47.3% of the subjects were sensitive to cross-reactive allergenic components, most often proteins of the lipocalin family (57.8%), followed by PR-10 (26.3%), PR-14 (21%) and PR-5 proteins (21%). asIgE against species-specific allergens were found in 10.5% of the study subjects. No statistically significant relationships were observed between the severity or duration of AD and the prevalence and levels of asIgE against the allergens included in the ISAC panel. However, duration of AD correlated significantly with the serum concentration of total IgE.ConclusionsThe ISAC test is suitable for determination of the allergenic phenotype in a given patient, and as such has an unquestioned diagnostic and therapeutic value.

β‐1,3‐glucanase rOle e 9 and MnSOD rAsp f 6 IgE reactivity are the signature of atopic dermatitis in the Mediterranean area

“…Patients affected by severe AD also differed from patients with moderate AD for several characteristics. In partial contrast with previous studies which were mainly performed on AD cohorts belonging to Northern Europe and therefore exposed to different environmental stimuli, we found a higher prevalence of IgE reactivity to several pollen sources (cypress, grasses, olive tree, pellitory) and to house dust mite, associated with a reduced overall prevalence of Fagales‐related molecules. Mittermann et al, on the contrary, found that PR‐10 molecules were associated with AD more commonly than grass pollen allergens rPhl p 1, rPhl p 5, rPhl p 2 and rPhl p 6.…”

“…39 Interestingly, all homologous components, with the exclusion of profilin and polcalcin, were significantly associated with the occurrence of AD, in accordance with previous studies. 32,38 We found, as already reported, 40 a very high frequency of hyper-reactivity to at least one nsLTPs in the allergic control population (33%). However, in the patients affected by severe AD, such frequency was almost double (ie 60%), thus representing the top-ranking panallergen reactivity observed, followed by SSP (46.8%), lipocalin (43.7%) and PR-10 (42.4%).…”

“…In keeping with some Authors, 35,36 we observed a significant relationship among total IgE levels and AD severity, though such a relationship was not confirmed by others. 37,38 We found that, in the vast majority of cases, AD patients showed a widespread state of pan-reactivity to multiple biological sources and cross-reactive components, even in participants with mild AD or without currently active disease, and only with a history of AD.…”

Sensitization to Molecular Components in 104 Atopic Dermatitis Patients in Relation to Subgroups of Patients Suffering from Bronchial Asthma and Allergic Rhinitis

“…Only few reports demonstrate the sensitization to molecular components in atopic dermatitis patients and the relation of this sensitization to the severity of atopic dermatitis, and to the occurrence of bronchial asthma and allergic rhinitis (8,17,18). It should be emphasized that in this study we focused on the degree of sensitization only and not on its clinical relevance.…”

Assessment of Google Trends terms reporting allergies and the grass pollen season in Ukraine

“…Grass pollen allergy was investigated due to the high prevalence in Europe. 10,11 The term "grass" as a plant requires its translation into the native language in order to investigate the grass pollen allergy pattern using GT. 12 There are also difficulties in the choice of the correct translation for "hay fever" and "rhinitis" in the Cyrillic-based languages.…”