Allergenic cross-reactivity between third stage larvae of Hysterothylacium aduncum and Anisakis simplex

Fernández-Caldas, Enrique; Quirce, Santiago; Marañó, F.; Gómez, María; Botella, Herminia Gijón; Román, Ramón López

doi:10.1016/s0091-6749(98)70364-1

Cited by 55 publications

(37 citation statements)

References 5 publications

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“…Cross-reactivity by IgE is of interest because it may reflect the pattern of clinical sensitivities (1). In this study, (13,26,34). In particular, it has been reported that IgE antibodies to tropomyosin could be induced during mite immunotherapy (35).…”

Section: Discussionmentioning

confidence: 91%

Allergenic Characterization of Tropomyosin from the Dusky Brown Cockroach,Periplaneta fuliginosa

Jeong

Hwang

Lee

et al. 2004

Clin Vaccine Immunol

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Household arthropods are one of the most common causes of allergic diseases. Four species of cockroaches are found to reside in Korean homes, but published work deals almost exclusively with the German and American cockroaches. This study was undertaken to investigate the cross-reactive allergenic components of the dusky brown cockroach, Periplaneta fuliginosa. Enzyme-linked immunosorbent assay (ELISA) inhibition and immunoblot analyses for the dusky brown cockroach were performed with Blattella germanica and Dermatophagoides farinae allergic sera. cDNA encoding tropomyosin, which is a well known cross-reactive panallergen, was cloned by reverse transcriptase PCR, and recombinant protein was produced by using a pET-28b expression system. Native tropomyosin was purified by ammonium sulfate fractionation and electroelution. The immunoglobulin E (IgE) reactivities of native and recombinant tropomyosins were compared by an ELISA inhibition study. All 30 sera tested showed P. fuliginosa-specific IgE, and the IgE-binding reactivity of the P. fuliginosa extract was inhibited as much as 79.4% by a B. germanica extract and as much as 63.3% by a D. farinae extract. The deduced amino acid sequence of cloned cDNA was identical with that of Periplaneta americana tropomyosin (98.5% nucleotide sequence identity). Seven of 26 (26.9%) allergic sera had IgE specific for recombinant protein, and the maximum inhibition of P. fuliginosa-specific IgE achieved with recombinant tropomyosin was 37.7% at an inhibitor concentration of 10 g/ml. Native tropomyosin inhibited the binding of IgE to the P. fuliginosa, B. germanica, and D. farinae extracts by 65.0, 51.8, and 39% at an inhibitor concentration of 1 g/ml. P. fuliginosa appears to possess allergens that are highly cross-reactive with allergens of B. germanica and D. farinae. Tropomyosin was found to be a major allergenic component accounting for the cross-reactivity between cockroaches and dust mites.

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Section: Discussionmentioning

confidence: 91%

Allergenic Characterization of Tropomyosin from the Dusky Brown Cockroach,Periplaneta fuliginosa

Jeong

Hwang

Lee

et al. 2004

Clin Vaccine Immunol

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“…After the report by Kasuya that allergies induced by the mackerel were actually the result of L3 contamination in the mackerel but not the mackerel itself, many researchers have detected species-specific antigens for the diagnosis of L3-dependent allergies (Yakunin and Hallenbeck, 1998; Asturias et al, 2000;Perez-Perez et al, 2000; Caballero and Moneo, 2002;Shimakura et al, 2004). These inquiries improved L3-dependent allergy diagnoses, and provided a simple method for the resolution of the cross-reactivity problem (Pascual et al, 1997;Fernandez-Caldas et al, 1998;Guilloux et al, 1998; Cho and Cho, 2000;Johansson et al, 2001). A thorough understanding of L3-dependent allergies requires the use of a variety of tissue preparations and in vivo reactions.…”

Section: Introductionmentioning

confidence: 99%

Immune reactions and allergy in experimental anisakiasis

Cho

Lee

2006

Korean J Parasitol

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Abstract:The third-stage larvae (L3) of the parasitic nematode, Anisakis simplex, have been implicated in the induction of hyperimmune allergic reactions in orally infected humans. In this work, we have conducted a review of an investigation into immune reactions occurring in animals experimentally infected with A. simplex L3. The patterns of serum antibody productions in the experimental animals against excretory-secretory products (ESP) of A. simplex L3 contributed to our current knowledge regarding specific humoral immune reactions in humans. In our review, we were able to determine that L3 infection of experimental animals may constitute a good model system for further exploration of immune mechanisms and allergy in anisakiasis of humans. worldwide (Mattiucci et al., 1997;Shih, 2004;Ugland et al., 2004). Many epidemics have been reported in Japan and Spain (Chai et al., 2005). Increasing reports of L3 infection in fish have been documented in South Korea, in which the consumption of raw marine fish is also popular (Chai et al., 1992;Im et al., 1995;Song et al., 1995Song et al., , 1999. Easy access to endoscopes and enhanced awareness of anisakiasis among clinicians has resulted in better reporting of morbidity resultant from L3 infection. L3 infection induces the production of specific antibodies and cytokines (Kennedy et al., 1988;Daschner et al., 2001;Nieuwenhuizen et al., 2006). Antibodies can be detected 2 weeks after infection, consistent with the time courses associated with other microorganisms. Analyses of specific antibody levels are generally irrelevant to the differential diagnosis of an acute state in cases of L3 infection, because the profound pain associated with L3 penetration begins only a few hours after the consumption of infected raw fish. However, antibody level measurements are helpful both in the differentiation of tumors from the granulomas formed by infiltrating L3 and in investigations of allergic diseases (Gutierrez and Cuellar, 2002;Kim et al., 2006).The production of IgE tends to increase during parasite infections, but the ultimate effects of IgE vary considerably, depending on the host-parasite relationships. Hyperimmune allergic reactions have been closely associated with IgE production. The infection of a parasite into its normal host tends to reduce the development of allergic responses, despite the associated upshift in IgE production (van den Biggelaar et al., 2000;Yazdanbakhsh et al., 2002). By contrast, the infection of T. canis in humans, an abnormal host, induces increased allergic reactions (Sharp and Olson, 1962;Sharghi et al., 2001). A. simplex L3 has also been shown to induce allergic diseases at a high rate, principally due to the fact that humans are not a regular host of this parasite (Audicana et al., 2002;Klimpel et al., 2004).Through investigations of allergic responses to L3 for a period of more than 10 years, several shared features have been identified, which indicate that immune reactions to L3 infection evidence similar patterns in humans and experimental...

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“…They are also responsible for anisakidosis and allergic response in humans (Fernández de Corres et al 1996, Del Pozo et al 1997, Fernández-Caldas et al 1998. In spite of their importance, only few data are available regarding the biology of these parasites.…”

Section: Introductionmentioning

confidence: 99%

In vitro development of the fish parasite Hysterothylacium aduncum from the third larval stage recovered from a host to the third larval stage hatched from the egg

Adroher¹,

Malagón²,

Valero³

et al. 2004

Dis. Aquat. Org.

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Anisakids are parasitic nematodes of fish worldwide, producing economic and human health concerns. It is thus important to ascertain their in vitro life cycle in laboratory studies. Here we describe the in vitro development of third-stage larvae (L3) of Hysterothylacium aduncum isolated from blue whiting Micromesistius poutassou, to the hatching of L3 from eggs obtained from H. aduncum worms grown in GLIT medium (a modified mixture of Yaeger's LIT [Liver Infusion Tryptose] and Grace's media) at pH 4.0, 13°C and with 5% CO 2 in air. Under these conditions, L3 recovered from fish developed to mature adults (3.4 to 6.2 cm in length), with oviposition starting from Day 26 in culture. Fertilized eggs (mean size 64 × 52 µm) had a thick, rugose eggshell and were larger than unfertilized ones (mean size 49 × 42 µm), whose eggshells were thin and smooth. Eggs laid during the first and second week of oviposition, and maintained in 2.8% NaCl solution at 13°C, developed to L3. Under these maintenance conditions, between 20.6 and 52.5% of the eggs laid during the first week developed into larvae. Motile larvae, enclosed in a sheath, hatched from between 2 and 11% of these eggs. The larvae started to hatch 23 d after deposition. These larvae were 144 to 215 µm in length, enclosed in a 237 to 305 µm-long sheath. This GLIT culture medium may help to study the biology of this and other anisakids.KEY WORDS: Fish parasite · Hysterothylacium aduncum · Nematoda · Anisakidae · In vitro culture · Egg development · Hatching Resale or republication not permitted without written consent of the publisherDis Aquat Org 58: [41][42][43][44][45] 2004 mesistius poutassou (blue whiting), family Gadidae, purchased from the fish market of Granada (southern Spain). Blue whiting, on the Atlantic and Mediterranean Spanish coasts, are frequently parasitized by H. aduncum (Ruiz-Valero et al. 1992, Valero et al. 2000. The worms, found free in the host body cavity, were collected with the help of a needle with a blunt tip, placed on a Petri dish and washed in 0.9% NaCl solution several times. The worms were observed individually under an inverted microscope, and those that showed any kind of internal or external damage were discarded. They were then identified according to morphological features (Hartwich 1975, Petter & Maillard 1988, Petter & Cabaret 1995.Prior to cultivation, each larva was placed in an antibiotic-antifungal solution (80 mg gentamicin sulfate, 0.625 mg amphotericin B, 10 000 IU sodium penicillin G, 10 mg streptomycin sulfate and 4.5 ml Hanks' solution, for a final solution volume of 10 ml) and axenized as described elsewhere (Iglesias et al. 1997, Iglesias et al. 2001. Worms were cultured in sterile polystyrene 30 ml flasks. The culture medium (10 ml) was placed into each flask with 8 to 10 parasites. The culture flasks were then placed in an incubator at 13°C and 5% CO 2 in humid air, and the culture medium was renewed once a week. The worms were observed daily for motility, moulting and survival. The culture medium (GLI...

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Allergenic cross-reactivity between third stage larvae of Hysterothylacium aduncum and Anisakis simplex

Cited by 55 publications

References 5 publications

Allergenic Characterization of Tropomyosin from the Dusky Brown Cockroach,Periplaneta fuliginosa

Allergenic Characterization of Tropomyosin from the Dusky Brown Cockroach,Periplaneta fuliginosa

Immune reactions and allergy in experimental anisakiasis

In vitro development of the fish parasite Hysterothylacium aduncum from the third larval stage recovered from a host to the third larval stage hatched from the egg

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