Alkaloid profile and antimicrobial activity of Lupinus angustifolius L. alkaloid extract

Abstract

Purpose of the present study was to evaluate alkaloid profile of the aerial parts of Lupinus angustifolius growing in Turkey by capillary gas chromatography-mass spectrometry (GC-MS). Fifteen alkaloids were identified by capillary GC-MS. 13a-Hydroxylupanine (50.78%) and lupanine (23.55%) were determined as the main alkaloids in the aerial parts of L. angustifolius. Ammodendrine, isoangustifoline, tetrahydrorhombifoline, angustifoline, a-isolupanine, 5,6-dehydrolupanine, 11,12-dehydrolupanine, 13a-acetoxylupanine, 13a-isovaleroyloxylupanine, 13a-valeroyloxylupanine, 13a-tigloyloxylupanine, 13a-cis-cinnamoyloxylupanine and 13a-cis-cinnamoyloxy-17-oxolupanine were identified as the minor alkaloids of the plant. Furthermore, antibacterial and antifungal activities of L. angustifolius alkaloid extract were tested against standard strains of the following bacteria; Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis and Staphylococcus aureus as well as the fungi; Candida albicans and C. krusei. The alkaloid extract showed significant activity on B. subtilis, S. aureus and P. aeruginosa while it was weakly active on E. coli. On the other hand, the extract possessed moderate activity against C. albicans and C. krusei.

Criteria for Safe Use of Plant Ingredients in Diets for Aquacultured Fish

“…In contrast, Erdemoglu et al (2007) recently reported significant in vitro antibacterial activity of alkaloid extracts from L. angustifolius against Staphylococcus aureus, B. subtilis, and Pseudomonas aeruginosa, and moderate antifungal activity against Candida albicans and Candida krusei. Therefore, there is the potential for lupin alkaloids to affect the intestinal microbiota but there are currently no studies in the scientific literature specifically addressing antimicrobial effects in vivo.…”

Total phenolic content, FTIR analysis, and antiproliferative evaluation of lupin seeds harvest from western Romania

“…Other chemical constituents worth mentioning, but present only in a concentration under 5%, include oleic and linoleic acids, flavonoids, isoflavonoids, carotenoids, polysaccharides, alkaloids. The amount of the above-mentioned constituents can vary depending on the environment, pedoclimatic and genetic conditions [4][5][6]. It is also very important to mention that from the point of view of the amount of protein, lupine seeds follow closely soybean seeds, and as a particular advantage, Lupinus spp.…”

“…Lampart-Szczapa et al, have described the antibacterial activity of lupin seeds extracts on both Gram (+) and Gram (-) strains, and concluded that it is directly correlated with the amount of total polyphenols [8]. Erdemoglu et al, have shown that the alkaloid extract obtained from Lupinus angustifolius L. possesses significant antibacterial and antimitotic activity against B. subtilis, S. aureus, P. aeruginosa, C. albicans and C. kruse, and has a weak potential against E.coli [6]. The group of Sirtori et al, have demonstrated that proteins extracted from white lupin seeds present cholesterol-lowering effects in rats, and augment the activity of the LDL receptor in the human hepatoma HepG2 cell line [9].…”

Antioxidant and antibacterial activities of leaves and branches extracts of Tecoma stans (L.) Juss. ex Kunth against nine species of pathogenic bacteria

“…On the other hand, the presence of alkaloids in T. stans have been shown to possess an antimicrobial and antioxidant activities (Erdemoglu et al, 2007;MaizaBenabdesselam et al, 2007). In our study, the CHCl 3 fraction which contains the precipitated alkaloids was found to own a potential activity against the tested bacterial strains.…”