Aligned carbon nanotubes on quartz substrate for liquid gated biosensing

Palaniappan, Alagappan; Goh, Wei Hau; Tey, Ju Nie; Wijaya, I. Putu Mahendra; Moochhala, Shabbir; Liedberg, Bo; Mhaisalkar, Subodh

doi:10.1016/j.bios.2010.01.009

Cited by 42 publications

(29 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The nanotubes are made sensitive to biomarkers by chemical functionalization of antibodies to the nanotube sidewalls. Many reports have described carbon nanotube sensors for cancer biomarkers as free proteins 11–15 , for specific DNA sequences 16,17 and other molecules of interest 18–20 . For many of these, the sensing mechanism is the gating effect of the target proteins as they bind to the antibodies, bringing them near the CNT 21,22 , within the debye length of the fluid.…”

Section: Introductionmentioning

confidence: 99%

“…For many of these, the sensing mechanism is the gating effect of the target proteins as they bind to the antibodies, bringing them near the CNT 21,22 , within the debye length of the fluid. Positively charged proteins switch off semiconducting p-type CNT, decreasing device conductance 11,13–15 . Negatively charged proteins switch on the CNTs, increasing the conductance 12 .…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Electrical detection of specific versus non-specific binding events in breast cancer cells

et al. 2012

View full text Add to dashboard Cite

Detection of circulating tumor cells (CTCs) from patient blood samples offers a desirable alternative to invasive tissue biopsies for screening of malignant carcinomas. A rigorous CTC detection method must identify CTCs from millions of other formed elements in blood and distinguish them from healthy tissue cells also present in the blood. CTCs are known to overexpress certain surface receptors, many of which aid them in invading other tissue, and these provide an avenue for their detection. We have developed carbon nanotube (CNT) thin film devices to specifically detect these receptors in intact cells. The CNT sidewalls are functionalized with antibodies specific to Epithelial Cell Adhesion Molecule (EpCAM), a marker overexpressed by breast and other carcinomas. Specific binding of EpCAM to anti-EpCAM causes a change in the local charge environment of the CNT surface which produces a characteristic electrical signal. Two cell lines are tested in the device: MCF7, a mammary adenocarcinoma line which overexpresses EpCAM, and MCF10A, a non-tumorigenic mammary epithelial line which does not. Introduction of MCF7s causes significant changes in the electrical conductance of the devices due to specific binding and associated charge environment change near the CNT sidewalls. Introduction of MCF10A displays a different profile due to purely nonspecific interactions. The profile of specific vs. nonspecific interaction signatures using carbon based devices will guide development of this diagnostic tool towards clinical sample volumes.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Electrical detection of specific versus non-specific binding events in breast cancer cells

et al. 2012

View full text Add to dashboard Cite

show abstract

“…This indicates that the electrical characteristics of CNT fieldeffect transistors in a buffer solution are susceptible to noise. 10) Recently, network CNTs 3,8) or aligned CNT 11) devices have been investigated for the sensors to solve these problems. However, it is difficult to grow only semiconducting CNTs at present.…”

Section: Introductionmentioning

confidence: 99%

Label-Free Aptamer-Based Immunoglobulin Sensors Using Graphene Field-Effect Transistors

Ohno

Maehashi

Inoue

et al. 2011

Jpn. J. Appl. Phys.

View full text Add to dashboard Cite

Electrical detection of specific proteins was demonstrated using aptamer-modified graphene field-effect transistors (G-FETs). Immunoglobulin E (IgE) aptamers were immobilized onto the graphene surface with 1-pyrenebutanoic acid succinimidyl ester as a linker. From an atomic-force microscopy image, the height of the graphene channel was determined to be approximately 3 nm, indicating the successful functionalization of aptamers. The slope of the transport characteristics before and after aptamer functionalization did not change, indicating that the functionalization process was carried out without introducing defects. The aptamer-modified G-FET successfully detected only the target protein while the drain current of the bare G-FETs changed by various proteins. These results suggest that the binding of the non-target protein to the graphene channel surface was sufficiently suppressed. #

show abstract

“…Positively charged proteins switch off semiconducting p-type CNT, decreasing device conductance [51], [53], [60], [61]. Negatively charged proteins switch on the CNTs, 13 increasing the conductance [52].…”

Section: Sensing Mechanismsmentioning

confidence: 99%

“…Carbon nanotube biosensors have been designed to detect DNA [41][42][43][44][45], glucose [43], [45][46][47], enzymes [45], [48], [49], protein-ligand interactions [50], and cancer biomarkers [51][52][53][54][55]. Regarding cancer biomarkers, carbon nanotube biosensors have, to this point, mainly been developed for free proteins whose elevated levels in blood serum also indicate cancer.…”

Section: Sensing Mechanismsmentioning

confidence: 99%

Carbon nanotube biosensors for detection of biomarkers in breast cancer cells.

King¹

View full text Add to dashboard Cite

Aligned carbon nanotubes on quartz substrate for liquid gated biosensing

Cited by 42 publications

References 27 publications

Electrical detection of specific versus non-specific binding events in breast cancer cells

Electrical detection of specific versus non-specific binding events in breast cancer cells

Label-Free Aptamer-Based Immunoglobulin Sensors Using Graphene Field-Effect Transistors

Carbon nanotube biosensors for detection of biomarkers in breast cancer cells.

Contact Info

Product

Resources

About