Interfacial water constitutes a formidable barrier to strong surface bonding, hampering the development of water-resistant synthetic adhesives. Notwithstanding this obstacle, the Asian green mussel Perna viridis attaches firmly to underwater surfaces via a proteinaceous secretion (byssus). Extending beyond the currently known design principles of mussel adhesion, here we elucidate the precise time-regulated secretion of P. viridis mussel adhesive proteins. The vanguard 3,4-dihydroxy-L-phenylalanine (Dopa)-rich protein Pvfp-5 acts as an adhesive primer, overcoming repulsive hydration forces by displacing surface-bound water and generating strong surface adhesion. Using homology modelling and molecular dynamics simulations, we find that all mussel adhesive proteins are largely unordered, with Pvfp-5 adopting a disordered structure and elongated conformation whereby all Dopa residues reside on the protein surface. Time-regulated secretion and structural disorder of mussel adhesive proteins appear essential for optimizing extended nonspecific surface interactions and byssus' assembly. Our findings reveal molecular-scale principles to help the development of wet-resistant adhesives.
This letter demonstrates a biosensing platform for naked eye detection of miRNA, fabricated using a poly(vinylidene fluoride) thin paper impregnated with positively charged poly(3-alkoxy-4-methylthiophene) as luminescent reporters. The miRNA assay is based on the formation of a duplex and a triplex species between the "reporter and miRNA" and "reporter and miRNA-peptide nucleic acid (PNA) hybrid", which yields two significantly different optical signals, thereby facilitating naked eye detection. This letter illustrates the successful validation of the proposed methodology via a mir21 assay (miRNA sequence associated with lung cancer). Furthermore, this facile platform enables rapid, sensitive, and selective detection of miRNA, at clinically relevant concentration levels as well as single base pair mismatch, without requiring complex and expensive instrumentation.
Peptide-functionalized
gold nanoparticles (AuNPs) are extensively
utilized in colorimetric assays for rapid and sensitive detection
of various biomedical and environmental targets. Although extensively
used as colorimetric reporting systems, the role of the size and concentration
of the AuNPs has not been thoroughly investigated. In this study,
a 12-mer cardiac troponin I (cTnI)-specific peptide CALNN-Peg4-FYSHSFHENWPS was immobilized on AuNPs of
different size and concentration via the CALNN anchoring sequence.
A relationship was established between the total surface area of the
AuNPs (binding availability) and response (centroid shift). Moreover,
a colorimetric assay for cTnI operating under optimized conditions
(36 nm AuNPs) yielded a limit of detection of 0.2 ng/mL (8.4 pM) when
tested in diluted serum samples with an assay time of 10 min. This
encouraging result opens up for further development of AuNP assays
in early diagnosis of cardiac injury.
Nature has evolved several molecular strategies to ensure adhesion in aqueous environments, where artificial adhesives typically fail. One recentlyunveiled molecular design for wet-resistant adhesion is the cohesive cross-β structure characteristic of amyloids, complementing the well-established surface-binding strategy of mussel adhesive proteins based on 3,4-l-dihydroxyphenylalanine (Dopa). Structural proteins that self-assemble into cross β-sheet networks are the suckerins discovered in the sucker ring teeth of squids. Here, light is shed on the wet adhesion of cross-β motifs by producing recombinant suckerin-12, naturally lacking Dopa, and investigating its wet adhesion properties. Surprisingly, the adhesion forces measured on mica reach 70 mN m −1 , exceeding those measured for all mussel adhesive proteins to date. The pressure-sensitive adhesion of artificial suckerins is largely governed by their cross-β motif, as evidenced using control experiments with disrupted cross-β domains that result in complete loss of adhesion. Dopa is also incorporated in suckerin-12 using a residue-specific incorporation strategy that replaces tyrosine with Dopa during expression in Escherichia coli. Although the replacement does not increase the long-term adhesion, it contributes to the initial rapid contact and enhances the adsorption onto model oxide substrates. The findings suggest that suckerins with supramolecular cross-β motifs are promising biopolymers for wet-resistant adhesion.
The only nonthermal method of depositing a bioceramic-based coating on polymeric substrates is by incubation in liquid, e.g., simulated body fluid to form an apatite-like layer. The drawbacks of this method include the long processing time, the production of low scratch resistant coating, and an end product that does not resemble the intended bioceramic composition. Techniques, such as plasma spraying and magnetron sputtering, involving high processing temperature are unsuitable for polymers, e.g., PMMA. Here, we introduce a nonthermal coating method to immobilize hydroxyapatite (HAp) and TiO nanoparticles on PMMA via a simple and fast dip coating method. Cavities that formed on the PMMA, induced by chloroform, appeared to trap the nanoparticles which accumulated to form layers of bioceramic coating only after 60 s. The resulting coating was hydrophilic and highly resistant to delamination. In the context of our research and to address the current clinical need, we demonstrate that the HAp-coated PMMA, which is intended to be used as a visual optic of a corneal prosthetic device, improves its bonding and biointegration with collagen, the main component of a corneal stroma. The HAp-coated PMMA resulted in better adhesion with the collagen than untreated PMMA in artificial tear fluid over 28 days. Human corneal stromal fibroblasts showed better attachment, viability, and proliferation rate on the HAp-coated PMMA than on untreated PMMA. This coating method is an innovative solution to immobilize various bioceramic nanoparticles on polymers and may be used in other biomedical implants.
The enhancement of upconversion luminescence of lanthanide-ion doped fluoride upconversion nanoparticles (UCNPs) is particularly important and highly required for their myriad applications in sensing, photoelectronic devices and bio-imaging. In this work, the amplification of luminescence in NaYF4:Yb/Er and NaYF4:Yb/Tm UCNPs in close proximity to the three-dimensional photonic crystal (3D PC) surface for improved near-infrared photoresponse of a carbon nanotube-based phototransistor is reported. The self-assembled opal 3D PCs with polystyrene sphere sizes of 200, 290 and 360 nm that exhibit reflection peaks of 450, 650 and 800 nm respectively were used for upconversion enhancement, and around 30 times enhancement was obtained for NaYF4:Yb/Er and NaYF4:Yb/Tm UCNPs. Time-resolved upconversion emission and 3D PC transmittance-dependent upconversion enhancement reveal that the enhanced absorption and the extraction effects, resulting from the enhanced non-resonant pump excitation field and the strong coherent scattering provided by 3D PCs respectively, are responsible for the large enhancement. As a proof-of-concept experiment, the prepared 3D PC/NaYF4:Yb/Tm UCNP coupled material layer was introduced into the carbon nanotube-based phototransistor. It is shown that the photoresponsivity of the device to near-infrared light was improved by 10 times with respect to the control device with carbon nanotubes only, which reveals the promising applications of this coupled material in photoelectronic devices such as photovoltaics and other types of phototransistors.
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