Label-Free Aptamer-Based Immunoglobulin Sensors Using Graphene Field-Effect Transistors

Ohno, Yasuhide; Maehashi, Kenzo; Inoue, Kōichi; Matsumoto, Kazuhiko

doi:10.1143/jjap.50.070120

Cited by 38 publications

(35 citation statements)

References 34 publications

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“…Among all the potential applications we can envisage those requiring high selectivity, as those related to bio-sensing or bio-field-effect transistors10212728. The redistribution of charge that can be expected upon adsorption on a target immobilized by our protocol could lead to a change in the electric field across the device, inducing changes in the electronic conductivity and the overall device response20. Our protocol could be also compatible with other hetero-applications, in which one could combine the graphene properties requiring low-sheet resistance, as solar cells, light-emitting diodes, liquid-crystal displays, touch screens, with other functionalities provided by the new chemical groups.…”

Section: Discussionmentioning

confidence: 99%

Highly selective covalent organic functionalization of epitaxial graphene

et al. 2017

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Graphene functionalization with organics is expected to be an important step for the development of graphene-based materials with tailored electronic properties. However, its high chemical inertness makes difficult a controlled and selective covalent functionalization, and most of the works performed up to the date report electrostatic molecular adsorption or unruly functionalization. We show hereafter a mechanism for promoting highly specific covalent bonding of any amino-terminated molecule and a description of the operating processes. We show, by different experimental techniques and theoretical methods, that the excess of charge at carbon dangling-bonds formed on single-atomic vacancies at the graphene surface induces enhanced reactivity towards a selective oxidation of the amino group and subsequent integration of the nitrogen within the graphene network. Remarkably, functionalized surfaces retain the electronic properties of pristine graphene. This study opens the door for development of graphene-based interfaces, as nano-bio-hybrid composites, fabrication of dielectrics, plasmonics or spintronics.

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Section: Discussionmentioning

confidence: 99%

Highly selective covalent organic functionalization of epitaxial graphene

et al. 2017

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“…Note that the transfer curves of these sensor devices exhibit a low on/off current ratio (or resistor‐like behavior); hence, the detection was mainly based on the device conductivity change 25, 27. Since the conductivity of graphene materials is very sensitive to the scattering process from the Coulomb charges including the DNA molecules and the ions in buffer solution,28 unambiguous detection of DNA hybridization based on the conductivity change may require more specific measurement conditions to demonstrate reasonable reproducibility. Meanwhile, it is speculated that the interaction between rGO and DNA molecules strongly depends on the size or defect content of the rGO sheets, which in turns affects the detection performance 27.…”

Section: Introductionmentioning

confidence: 99%

Label‐Free Electrical Detection of DNA Hybridization on Graphene using Hall Effect Measurements: Revisiting the Sensing Mechanism

Lin

Loan

Chen

et al. 2013

Adv Funct Materials

118

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There is broad interest in using graphene or graphene oxide sheets as a transducer for label-free and selective electrical detection of biomolecules such as DNA. However, it is still not well explored how the DNA molecules interact with and infl uence the properties of graphene during the detection. Here, Hall effect measurements based on the Van der Pauw method are used to perform single-base sequence selective detection of DNA on graphene sheets, which are prepared by chemical vapor deposition. The sheet resistance increases and the mobility decreases with the addition of either complementary or one-base mismatched DNA to the graphene device. The hole carrier concentration of the graphene devices increases signifi cantly with the addition of complementary DNA but it is less affected by the one-base mismatched DNA. It is concluded that the increase in hole carrier density, indicating p-doping to graphene, is better correlated with the DNA hybridization compared to the commonly used parameters such as conductivity change. The different electrical observations of p-doping from Hall effect measurements and n-doping from electrolyte-gated transistors can be explained by the characteristic morphology of partially hybridized DNA on graphene and the mismatch between DNA chain length and Debye length in electrolytes.

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“…Sensor revealed good selectivity demonstrated in experiment when IgE has been replaced by BSA, human IgA and IgM. Field effect transistors (FET|) has also been used for immobilization of aptamers on a various support, such as carbon nanotubes or graphene . Addition of IgE affected the drain current of FET that has been used for IgE detection.…”

Section: Immunosensors and Aptasensors For Ige Detectionmentioning

confidence: 94%

Affinity Biosensors for Detection Immunoglobulin E and Cellular Prions. Antibodies vs. DNA Aptamers

Hianik

2016

Electroanalysis

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Immunosensors for detection of proteins are of high importance for medical diagnostics. There exists continuous effort in their development using new methods of antibody immobilizations at the various surfaces including novel nanomaterials, such as carbon nanotubes, graphene, dendrimers and combination of these materials with nanoparticles of various origin. At the same time as an alternative to antibodies DNA or RNA aptamers are considered as novel receptors during last 2 decades. In contrast with antibodies aptamers are more flexible and stable, allowing various chemical modification without lost of their sensitivity. This review compares the properties of existing immuno‐ and aptasensors for detection human immunoglobulin E (IgE) and cellular prions (PrPC). It has been shown, that both immuno‐ and aptasensors are of comparable sensitivity and selectivity that depends on the method of receptor immobilization and detection.

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Label-Free Aptamer-Based Immunoglobulin Sensors Using Graphene Field-Effect Transistors

Cited by 38 publications

References 34 publications

Highly selective covalent organic functionalization of epitaxial graphene

Highly selective covalent organic functionalization of epitaxial graphene

Label‐Free Electrical Detection of DNA Hybridization on Graphene using Hall Effect Measurements: Revisiting the Sensing Mechanism

Affinity Biosensors for Detection Immunoglobulin E and Cellular Prions. Antibodies vs. DNA Aptamers

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