Algorithms for the study of episodic hormone secretion

Merriam, George R.; Wachter, Kenneth W.

doi:10.1152/ajpendo.1982.243.4.e310

Cited by 916 publications

(626 citation statements)

References 11 publications

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“…The 6 h serum profiles of prolactin, LH and testosterone were additionally analysed for pulsatility by means of the Pulsar 1 programme developed by [39]. The programme identifies hormone peaks by height and duration from a smoothed baseline, using the assay S.D.…”

Section: Statistical Evaluationmentioning

confidence: 99%

Effects of the dopamine agonist cabergoline on the pulsatile and TRH-induced secretion of prolactin, LH, and testosterone in male beagle dogs

Koch

Hoppen²,

Dieleman

et al. 2006

Theriogenology

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In the present study, the pulsatile serum profiles of prolactin, LH and testosterone were investigated in eight clinically healthy fertile male beagles of one to six years of age. Serum hormone concentrations were determined in blood samples collected at 15 min intervals over a period of 6 h before (control) and six days before the end of a four weeks treatment with the dopamine agonist cabergoline (5 mg kg À1 bodyweight/day). In addition, the effect of cabergoline administration was investigated on thyrotropin-releasing hormone (TRH)-induced changes in the serum concentrations of these hormones.In all eight dogs, the serum prolactin concentrations (mean 3.0 AE 0.3 ng ml À1 ) were on a relatively constant level not showing any pulsatility, while the secretion patterns of LH and testosterone were characterised by several hormone pulses. Cabergoline administration caused a minor but significant reduction of the mean prolactin concentration (2.9 AE 0.2 ng ml À1 , p < 0.05) and did not affect the secretion of LH (mean 4.6 AE 1.3 ng ml À1 versus 4.4 AE 1.7 ng ml À1 ) or testosterone (2.5 AE 0.9 ng ml À1 versus 2.4 AE 1.2 ng ml À1 ). Under control conditions, a significant prolactin release was induced by intravenous TRH administration (before TRH: 3.8 AE 0.9 ng ml À1 ,

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Section: Statistical Evaluationmentioning

confidence: 99%

Effects of the dopamine agonist cabergoline on the pulsatile and TRH-induced secretion of prolactin, LH, and testosterone in male beagle dogs

Koch

Hoppen²,

Dieleman

et al. 2006

Theriogenology

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“…The 6-h plasma profiles of GH were analyzed by means of the Pulsar program developed by Merriam and Wachter [26]. The program identifies secretory peaks by height and duration from a smoothed baseline, using the assay SD as a scale factor.…”

Section: Data Processing and Statistical Analysismentioning

confidence: 99%

Treatment of growth hormone excess in dogs with the progesterone receptor antagonist aglépristone

et al. 2006

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Acromegaly or hypersomatotropism in dogs is almost always due to progestin-induced hypersecretion of GH originating from the mammary gland. The aim of this study was to investigate whether aglépristone, a progesterone receptor antagonist, can be used to treat this form of canine acromegaly. In five Beagle bitches hypersomatotropism was induced by administration of MPA for over 1 year. Subsequently, aglépristone was administered. Blood samples were collected before MPA administration, immediately before, during, and 3.5 and 5.5 weeks after the last administration of aglépristone for determination of the plasma concentrations of GH and IGF-I. In addition, blood samples for the determination of the 6-h plasma profile of GH were collected before MPA administration, before aglépristone administration, and 1 week after the last aglépristone treatment.MPA administration resulted in a significant increase of the mean plasma IGF-I concentration, whereas analysis of the pulsatile plasma profile demonstrated a trend (P = 0.06) for a higher mean basal plasma GH concentration and a higher mean AUC 0 for GH. Treatment with aglépristone resulted in a significant decrease of the mean plasma GH and IGF-I concentrations. Analysis of the pulsatile plasma profile showed a trend (P = 0.06) for a lower mean basal plasma GH concentration and a lower mean AUC 0 for GH 1 week after the last aglépristone treatment compared with these values before aglépristone administration. Three and a half and 5.5 weeks after the last aglépristone administration the mean plasma IGF-I concentration increased again.In conclusion, aglépristone can be used successfully to treat dogs with progestin-induced hypersomatotropism. #

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“…Individual profiles of plasma E2, PRL and cortisol, and salivary cortisol were analyzed according to the method described by Merriam and Wachter [22]. The baseline was evaluated at each point using a weighted regression calculated on the 72 h period surrounding the considered point.…”

Section: Data Processing and Statisticsmentioning

confidence: 99%

Hormones, IgG and lactose changes around parturition in plasma, and colostrum or saliva of multiparous sows

et al. 2004

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-Blood, colostrum and saliva samples were serially taken from 6 multiparous sows from day 109 of gestation until day 3 postpartum. Plasma was assayed for oestradiol-17β (E2), progesterone (P4), prolactin (PRL), cortisol, immunoglobulin G (IgG) and lactose. Colostrum was assayed for E2, P4, IgG and lactose. Lactoserum, obtained after ultra centrifugation of colostrum, was assayed for PRL. Saliva was assayed for cortisol. Time-related variations in hormone, IgG and lactose concentrations measured in plasma were parallel to those measured in colostrum, lactoserum or saliva. However, the concentrations were higher in colostrum or lactoserum and lower in saliva than in plasma. Ratios of concentrations of cortisol in saliva and PRL in lactoserum over those in plasma did not vary with time and averaged 0.2 and 1.6, respectively. Conversely, the ratios of concentrations of E2 and P4 in colostrum over those in plasma varied with time (P < 0.05) but were quite constant before the end of parturition, averaging 2.7 and 3.6, respectively. The ratios of concentrations of IgG and lactose in colostrum over those in plasma also varied with time (P < 0.05). The concentrations of hormones in plasma on the one hand and in colostrum, lactoserum or saliva on the other hand were significantly correlated but correlations varied with time (PRL across periods: r = 0.31; cortisol across periods: r = 0.60; E2 during parturition: r = 0.83; P4 before parturition: r = 0.82; P4 during parturition: r = 0.67). The present results indicate that around parturition, assays of hormones in colostrum or saliva can be used to study the hormonal status of sows. Furthermore, variations in colostrum and plasma concentrations of IgG and lactose are good indicators of the transition from colostrum to milk synthesis. sow / parturition / steroid / prolactin / lactose / immunoglobulins

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Algorithms for the study of episodic hormone secretion

Cited by 916 publications

References 11 publications

Effects of the dopamine agonist cabergoline on the pulsatile and TRH-induced secretion of prolactin, LH, and testosterone in male beagle dogs

Effects of the dopamine agonist cabergoline on the pulsatile and TRH-induced secretion of prolactin, LH, and testosterone in male beagle dogs

Treatment of growth hormone excess in dogs with the progesterone receptor antagonist aglépristone

Hormones, IgG and lactose changes around parturition in plasma, and colostrum or saliva of multiparous sows

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