Alginate-honey bioinks with improved cell responses for applications as bioprinted tissue engineered constructs

Abstract

Abstract

Recent progress in extrusion 3D bioprinting of hydrogel biomaterials for tissue regeneration: a comprehensive review with focus on advanced fabrication techniques

“…An ideal bioprinted skin should have specific characteristics such as biocompatibility, desired mechanical properties, proper surface chemistry, and be high porosity with a network of interconnected pores that will allow cells to attach and capability of transferring nutrients and eliminating wound exudates [42]. Accessible literature review reveals that a variety of biomaterials have been widely studied for the generation of skin grafts [91][92][93], in which the most common materials are hydrogels [94][95][96][97][98][99]. However, the commonly available natural polymers besides synthetic polymers cannot provide the complex microenvironment analogous with the natural ECM [27].…”

Advances in the Research of Bioinks Based on Natural Collagen, Polysaccharide and Their Derivatives for Skin 3D Bioprinting

“…This printed skin tissue construct closely mimicks the structure of native tissue ( Figure 5 B). Further research has also reported that the incorporation of honey as a bioink component can improve the biological properties of an alginate-based bioprinted construct [ 149 ]. A novel dermal substitute scaffold with sodium alginate/gelatin composite material as bioink via extrusion technique and three-stage crosslinking process has also been manufactured [ 29 ].…”

Importance of Alginate Bioink for 3D Bioprinting in Tissue Engineering and Regenerative Medicine

“…Also in another study, Christensen et al [12] printed vascular structures along with bifurcations (vertical and horizontal) in alginate and fibroblast of mouse bioinks. Blending alginate with other polymers (honey, gelatin) [22], amino acids like polyglutamic acid and poly-l-lysine [13,23], and some drugs like N-acetylcysteine (NAC) [24] was studied for improving the erratic degradation, cell-material interaction, cell viability, etc. The printer (inkjet) used CaCl 2 cross-linking agent supporting material for cross-linking the alginate bioink.…”

“…To back up the buoyant force in the regions overhanging in both vertical and horizontal printing and also supporting the regions spanning in the horizontal printing, their modified solution was used. Blending alginate with other polymers (honey, gelatin) [22], amino acids like (polyglutamic acid and poly-l-lysine) [13,23], and some drugs like N-acetylcysteine (NAC) [24] was studied for improving the erratic degradation, cell-material interaction, cell viability, etc. Jia et al [6] in their study showed the controlled degradation of oxidized alginate in 3D bioprinting.…”