Importance of Alginate Bioink for 3D Bioprinting in Tissue Engineering and Regenerative Medicine

Datta, Sudipto; Barua, Ranjit; Das, Jonali

doi:10.5772/intechopen.90426

Cited by 25 publications

(19 citation statements)

References 50 publications

(59 reference statements)

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“…Previous studies have shown that eggwhite is a biocompatible, non-toxic biomaterial [14] and that alginate is a commonly used biomaterial in cell printing [44]. Our present study examined the HUVEC viability in the EW-2%Alg printed construct post-bioprinting, which could be affected by the mechanical forces that the cells experienced during the bioprinting process.…”

Section: Discussionmentioning

confidence: 98%

Bioprinting and In Vitro Characterization of an Eggwhite-Based Cell-Laden Patch for Endothelialized Tissue Engineering Applications

Delkash

Gouin

Rimbeault

et al. 2021

JFB

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Three-dimensional (3D) bioprinting is an emerging fabrication technique to create 3D constructs with living cells. Notably, bioprinting bioinks are limited due to the mechanical weakness of natural biomaterials and the low bioactivity of synthetic peers. This paper presents the development of a natural bioink from chicken eggwhite and sodium alginate for bioprinting cell-laden patches to be used in endothelialized tissue engineering applications. Eggwhite was utilized for enhanced biological properties, while sodium alginate was used to improve bioink printability. The rheological properties of bioinks with varying amounts of sodium alginate were examined with the results illustrating that 2.0–3.0% (w/v) sodium alginate was suitable for printing patch constructs. The printed patches were then characterized mechanically and biologically, and the results showed that the printed patches exhibited elastic moduli close to that of natural heart tissue (20–27 kPa) and more than 94% of the vascular endothelial cells survived in the examination period of one week post 3D bioprinting. Our research also illustrated the printed patches appropriate water uptake ability (>1800%).

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Section: Discussionmentioning

confidence: 98%

Bioprinting and In Vitro Characterization of an Eggwhite-Based Cell-Laden Patch for Endothelialized Tissue Engineering Applications

Delkash

Gouin

Rimbeault

et al. 2021

JFB

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“…The authors of a previous study observed a significant improvement of the healing process and monocyte stimulation, as well as higher cytokine levels, such as tumor necrosis factor-α and interleukin-6 [ 128 ]. It was also proven that alginate might be useful in reconstructive surgery and bone regeneration, e.g., alginate scaffolds helped in bone tissue formation [ 129 ].…”

Section: Materials Additives Used In Gel Systemsmentioning

confidence: 99%

A Review on the Adaption of Alginate-Gelatin Hydrogels for 3D Cultures and Bioprinting

et al. 2021

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Sustaining the vital functions of cells outside the organism requires strictly defined parameters. In order to ensure their optimal growth and development, it is necessary to provide a range of nutrients and regulators. Hydrogels are excellent materials for 3D in vitro cell cultures. Their ability to retain large amounts of liquid, as well as their biocompatibility, soft structures, and mechanical properties similar to these of living tissues, provide appropriate microenvironments that mimic extracellular matrix functions. The wide range of natural and synthetic polymeric materials, as well as the simplicity of their physico-chemical modification, allow the mechanical properties to be adjusted for different requirements. Sodium alginate-based hydrogel is a frequently used material for cell culture. The lack of cell-interactive properties makes this polysaccharide the most often applied in combination with other materials, including gelatin. The combination of both materials increases their biological activity and improves their material properties, making this combination a frequently used material in 3D printing technology. The use of hydrogels as inks in 3D printing allows the accurate manufacturing of scaffolds with complex shapes and geometries. The aim of this paper is to provide an overview of the materials used for 3D cell cultures, which are mainly alginate–gelatin hydrogels, including their properties and potential applications.

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“…The very porous and surface scaffolds were used to distribute bioactive glass homogenously. The presence on the surface of composite 7 International Journal of Polymer Science scaffolds of calcium phosphate deposits in vitro bioactivity [95]. The metabolism of fibroblast was boosted by bioactive glass.…”

Section: Synthetic Biopolymer-based Compositesmentioning

confidence: 99%

Additive Manufacturing of Biopolymers for Tissue Engineering and Regenerative Medicine: An Overview, Potential Applications, Advancements, and Trends

Veeman

Sai

Sureshkumar³

et al. 2021

International Journal of Polymer Science

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As a technique of producing fabric engineering scaffolds, three-dimensional (3D) printing has tremendous possibilities. 3D printing applications are restricted to a wide range of biomaterials in the field of regenerative medicine and tissue engineering. Due to their biocompatibility, bioactiveness, and biodegradability, biopolymers such as collagen, alginate, silk fibroin, chitosan, alginate, cellulose, and starch are used in a variety of fields, including the food, biomedical, regeneration, agriculture, packaging, and pharmaceutical industries. The benefits of producing 3D-printed scaffolds are many, including the capacity to produce complicated geometries, porosity, and multicell coculture and to take growth factors into account. In particular, the additional production of biopolymers offers new options to produce 3D structures and materials with specialised patterns and properties. In the realm of tissue engineering and regenerative medicine (TERM), important progress has been accomplished; now, several state-of-the-art techniques are used to produce porous scaffolds for organ or tissue regeneration to be suited for tissue technology. Natural biopolymeric materials are often better suited for designing and manufacturing healing equipment than temporary implants and tissue regeneration materials owing to its appropriate properties and biocompatibility. The review focuses on the additive manufacturing of biopolymers with significant changes, advancements, trends, and developments in regenerative medicine and tissue engineering with potential applications.

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Importance of Alginate Bioink for 3D Bioprinting in Tissue Engineering and Regenerative Medicine

Cited by 25 publications

References 50 publications

Bioprinting and In Vitro Characterization of an Eggwhite-Based Cell-Laden Patch for Endothelialized Tissue Engineering Applications

Bioprinting and In Vitro Characterization of an Eggwhite-Based Cell-Laden Patch for Endothelialized Tissue Engineering Applications

A Review on the Adaption of Alginate-Gelatin Hydrogels for 3D Cultures and Bioprinting

Additive Manufacturing of Biopolymers for Tissue Engineering and Regenerative Medicine: An Overview, Potential Applications, Advancements, and Trends

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