Alginate encapsulation supports the growth and differentiation of human primordial follicles within ovarian cortical tissue

Laronda, Monica M.; Duncan, Francesca E.; Hornick, Jessica E.; Xu, Min; Pahnke, Jennifer E; Whelan, Kelly A.; Shea, Lonnie D.; Woodruff, Teresa K.

doi:10.1007/s10815-014-0252-x

Cited by 112 publications

(101 citation statements)

References 78 publications

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“…Research tissue from these investigational protocols has led to a series of breakthroughs in in vitro follicle growth and in vitro maturation technologies. 17,19,23 For this particular study, following informed consent under IRB-approved protocols, all tissue designated for basic research was processed at Northwestern University (Chicago, IL). Ovarian tissue that was removed at local OC-NPC sites (Chicago, IL) was brought to the research laboratory within 2-4 hours of removal and processed immediately upon arrival.…”

Section: Human Ovarian Research Tissue Acquisitionmentioning

confidence: 99%

“…It has been shown that these transport conditions do not compromise the function of the primordial follicles. 17,[24][25][26][27] For the subset of research samples described in this study (N = 24), small pieces of fresh tissue (up to 10%) were fixed and processed for histological analysis as described below ( Supplementary Fig. S1B).…”

Section: Human Ovarian Research Tissue Acquisitionmentioning

confidence: 99%

“…15,16 To overcome the risk, methods are being developed to isolate immature follicles from fresh or cryopreserved ovarian tissue and grow them in vitro. 12,[17][18][19] Mature oocytes derived from this method could then be cryopreserved and used for ART to restore fertility.…”

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confidence: 99%

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Pediatric and Teen Ovarian Tissue Removed for Cryopreservation Contains Follicles Irrespective of Age, Disease Diagnosis, Treatment History, and Specimen Processing Methods

Duncan

Pavone

Gunn

et al. 2015

Journal of Adolescent and Young Adult Oncology

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Section: Human Ovarian Research Tissue Acquisitionmentioning

confidence: 99%

Section: Human Ovarian Research Tissue Acquisitionmentioning

confidence: 99%

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Pediatric and Teen Ovarian Tissue Removed for Cryopreservation Contains Follicles Irrespective of Age, Disease Diagnosis, Treatment History, and Specimen Processing Methods

Duncan

Pavone

Gunn

et al. 2015

Journal of Adolescent and Young Adult Oncology

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“…This would suggest that additional factors from either the follicle itself or from the surrounding stromal cells within the ovary are necessary for sustained growth. This idea is supported by publications that have demonstrated enhanced growth of primary and early preantral follicles in the presence of other follicles or ovarian stromal cells [24,25,[43][44][45][46][47][48][49]. Whether any of these stromal factors might be induced by GDF9 has not been investigated.…”

Section: Discussionmentioning

confidence: 99%

“…In mice, the system has been used to successfully grow multilayer secondary follicles to mature eggs that were fertilized and gave rise to viable, fertile pups [30]. Sustained growth of early follicles-specifically, late primary and early secondary follicles-to the antral stage has been achieved in vitro with organ culture [24,[43][44][45] or coculture of isolated follicles with ovarian stromal cells [46][47][48][49], mouse embryonic fibroblasts [50], multiple other follicles [25], or supplemented media [51]. Together, these findings suggest that follicle growth at the primary and early secondary stages is enhanced by, and perhaps dependent upon, factors from the surrounding ovarian environment in addition to the critical role played by the oocyte-derived factor GDF9 [6,7,[17][18][19][20][21][22][23].…”

Section: Introductionmentioning

confidence: 99%

Growth and differentiation factor 9 promotes oocyte growth at the primary but not the early secondary stage in three-dimensional follicle culture

Cook-Andersen

Curnow

et al. 2016

J Assist Reprod Genet

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Purpose Factors that differentially regulate oocyte and granulosa cell growth within the early preantral follicle and how these factors differ at each stage of follicle growth remain poorly understood. The aim of this study was to isolate and evaluate the effect of recombinant growth and differentiation factor 9 (GDF9) on oocyte and granulosa cell growth at the primary and early secondary stages of preantral follicle growth during in vitro culture. Methods Primary stage follicles (diameters of 50-89 μm) and early secondary stage follicles (diameters of 90-120 μm) were isolated from immature mice, and individual, intact follicles were cultured in vitro in the presence and absence of recombinant GDF9. The effects of GDF9 on follicle growth were determined by the assessment of changes in the follicle volume during culture. The growth of the granulosa cell and oocyte compartments of the follicles was evaluated separately at each stage. Results GDF9 significantly increased the growth of isolated follicles at both the primary and early secondary follicle stages. Independent evaluation of the granulosa cell and oocyte compartments revealed that, while GDF9 promoted granulosa cell growth at both stages of folliculogenesis, oocyte growth was stage specific. GDF9 promoted growth of the oocyte at the primary, but not the early secondary, follicle stage.Conclusions These findings demonstrate a stage-specific role for GDF9 in the regulation of oocyte and granulosa cell growth at the primary and early secondary stages of preantral follicle development.

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“Tissue Papers” from Organ‐Specific Decellularized Extracellular Matrices

Jakus

Laronda

Rashedi

et al. 2017

Adv Funct Materials

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109

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Using an innovative, tissue-independent approach to decellularized tissue processing and biomaterial fabrication, the development of a series of “tissue papers” derived from native porcine tissues/organs (heart, kidney, liver, muscle), native bovine tissue/organ (ovary and uterus), and purified bovine Achilles tendon collagen as a control from decellularized extracellular matrix particle ink suspensions cast into molds is described. Each tissue paper type has distinct microstructural characteristics as well as physical and mechanical properties, is capable of absorbing up to 300% of its own weight in liquid, and remains mechanically robust (E = 1–18 MPa) when hydrated; permitting it to be cut, rolled, folded, and sutured, as needed. In vitro characterization with human mesenchymal stem cells reveals that all tissue paper types support cell adhesion, viability, and proliferation over four weeks. Ovarian tissue papers support mouse ovarian follicle adhesion, viability, and health in vitro, as well as support, and maintain the viability and hormonal function of nonhuman primate and human follicle-containing, live ovarian cortical tissues ex vivo for eight weeks postmortem. “Tissue papers” can be further augmented with additional synthetic and natural biomaterials, as well as integrated with recently developed, advanced 3D-printable biomaterials, providing a versatile platform for future multi-biomaterial construct manufacturing.

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Alginate encapsulation supports the growth and differentiation of human primordial follicles within ovarian cortical tissue

Cited by 112 publications

References 78 publications

Pediatric and Teen Ovarian Tissue Removed for Cryopreservation Contains Follicles Irrespective of Age, Disease Diagnosis, Treatment History, and Specimen Processing Methods

Pediatric and Teen Ovarian Tissue Removed for Cryopreservation Contains Follicles Irrespective of Age, Disease Diagnosis, Treatment History, and Specimen Processing Methods

Growth and differentiation factor 9 promotes oocyte growth at the primary but not the early secondary stage in three-dimensional follicle culture

“Tissue Papers” from Organ‐Specific Decellularized Extracellular Matrices

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