Algae Mediated Green Fabrication of Silver Nanoparticles and Examination of Its Antifungal Activity against Clinical Pathogens

Abstract

Algae extract has the great efficiency to synthesize the silver nanoparticles as a green route. Brown seaweed mediates the synthesis of silver nanomaterials using extract of Sargassum longifolium. For the improved production of silver nanomaterials, some kinetic studies such as time incubation and pH were studied in this work. 10 mL of algal extract was added into the 1 mM AgNO 3 aqueous solution. The pH and reaction time range were changed and the absorbance was taken for the characterization of the nanoparticles at various time intervals, and the high pH level shows the increased absorbance due to the increased nanoparticles synthesis. The synthesized silver nanoparticles were characterized by Scanning Electron Microscope (SEM) showing that the shape of the material is spherical, and X-Ray Diffraction value obtained from range of (1 1 1) confirmed synthesized silver nanoparticles in crystalline nature. TEM measurement shows spherical shape of nanoparticles. The Fourier Transmittance Infrared spectrum (FT-IR) confirms the presence of biocomponent in the algae extract which was responsible for the nanoparticles synthesis. The effect of the algal mediated silver nanoparticles against the pathogenic fungi Aspergillus fumigatus, Candida albicans, and Fusarium sp. S. longifolium mediated synthesized silver nanoparticles shows cheap and single step synthesis process and it has high activity against fungus. This green process gives the greater potential biomedical applications of silver nanoparticles.

Toxicity of seaweed-synthesized silver nanoparticles against the filariasis vector Culex quinquefasciatus and its impact on predation efficiency of the cyclopoid crustacean Mesocyclops longisetus

“…Recently, Vinayaga Moorthi et al (2015) reported the insecticidal activity of silver nanoparticles synthesized using Sargassum muticum against the common castor butterfly Ariadne merione, outlining changes in the protein profile of hemolymph, morphology of hemocytes, and deteriorated midgut inclusions. Furthermore, Sargassum longifolium-synthesized silver nanoparticles have been found toxic against the pathogenic fungi Aspergillus fumigatus, Candida albicans, and Fusarium sp., even at low dosages (Rajeshkumar et al 2014). …”

Green Synthesis of Silver Nanoparticles Using Medicinal Plant Acalypha Indica Leaf Extracts and Its Application as an Antioxidant and Antimicrobial Agent Against Foodborne Pathogens

“…A positive control of ketoconazole disks and negative control of water was also added to the plate. The zone of inhibition was measured after incubation at room temperature for 24-48 h [16]. The relative percentage inhibition of the test fungal strains against positive control was calculated with respect to the given formula [17].…”

Screening of cyanobacteria and microalgae for their ability to synthesize silver nanoparticles with antibacterial activity

“…Nanoparticles are gaining reputation as multifaceted materials exhibiting novel or advanced characteristics compared to larger particles [31], [38], [45]. Smaller sized nanoparticles display higher surface-to-volume ratio; a feature vital to catalytic reactivity, thermal conductivity, antimicrobial activity, chemical steadiness, and non-linear optical performance [22].…”