Alcohol tolerance in Escherichia coli

Ingram, L. O.; Vreeland, N S; Eaton, Leslie C.

doi:10.1016/s0091-3057(80)80030-x

Cited by 42 publications

(15 citation statements)

References 18 publications

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“…We found that the mutant cells without Hfq were more sensitive to salt, ethanol, and Triton X-100 than the wild-type cells, suggesting that Hfq affects the membrane integrity of L. monocytogenes (10,24). The demand for Hfq during environmental stress was further supported by the increase in hfq transcription when cells were exposed to various stress conditions, including salt and ethanol, and during entry into the stationary phase.…”

Section: Discussionmentioning

confidence: 81%

The RNA-Binding Protein Hfq ofListeria monocytogenes: Role in Stress Tolerance and Virulence

et al. 2004

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In gram-negative bacteria, the RNA-binding protein Hfq has emerged as an important regulatory factor in a variety of physiological processes, including stress resistance and virulence. In Escherichia coli, Hfq modulates the stability or the translation of mRNAs and interacts with numerous small regulatory RNAs. Here, we studied the role of Hfq in the stress tolerance and virulence of the gram-positive food-borne human pathogen Listeria monocytogenes. We present evidence that Hfq is involved in the ability of L. monocytogenes to tolerate osmotic and ethanol stress and contributes to long-term survival under amino acid-limiting conditions. However, Hfq is not required for resistance to acid and oxidative stress. Transcription of hfq is induced under various stress conditions, including osmotic and ethanol stress and at the entry into the stationary growth phase, thus supporting the view that Hfq is important for the growth and survival of L. monocytogenes in harsh environments. The stress-inducible transcription of hfq depends on the alternative sigma factor B , which controls the expression of numerous stress-and virulence-associated genes in L. monocytogenes. Infection studies showed that Hfq contributes to pathogenesis in mice, yet plays no role in the infection of cultured cell lines. This study provides, for the first time, information on the role of Hfq in the stress tolerance and virulence of a gram-positive pathogen.

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Section: Discussionmentioning

confidence: 81%

The RNA-Binding Protein Hfq ofListeria monocytogenes: Role in Stress Tolerance and Virulence

et al. 2004

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“…Ethanol is known to alter the fatty acid content of membranes [11], thus we speculate that disruption of both the LisRK system and the RR96 improves the resistance to ethanol by altering the membrane composition. This alteration could potentially also a¡ect the in vivo survival since both mutants demonstrated a reduced survival in mice when assayed by intragastric inoculation.…”

Section: Discussionmentioning

confidence: 88%

Listeria monocytogenes response regulators important for stress tolerance and pathogenesis

Kallipolitis¹

2001

FEMS Microbiology Letters

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Environmental sensing by two-component signal transduction systems is likely to play a role for growth and survival of Listeria monocytogenes both during transmission in food products and within a host organism. Two-component systems typically consist of a membrane-associated sensor histidine kinase and a gene regulatory protein, the response regulator (RR). We have identified seven putative RR genes in L. monocytogenes LO28 by PCR using degenerate oligonucleotide primers. By insertional inactivation we obtained data suggesting that three of the putative RRs contribute to the pathogenicity of L. monocytogenes in mice. Strikingly, the mutants that were attenuated in virulence also had a decreased ability to grow in the presence of various stress conditions potentially encountered in an infection process. Thus, our data point to a connection between the ability of the putative two-component systems to sense and respond to certain environmental stimuli, and the virulence of L. monocytogenes. ß 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

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“…However, they exhibit an abnormally long lag phase before becoming accommodated to the stress (Mitra et al, 1975). Alcohol tolerance in E. coli has also been extensively studied (Ingram, 1976;Ingram et al, 1980). Ethanol-induced changes in the membrane composition of E. coli appear to be beneficial for growth and survival (Dombek and Imgram, 1984).…”

Section: Discussionmentioning

confidence: 99%