Impact of Lead Stress and Adaptation in Escherichia coli

Kumar, Manoj; Upreti, Raj K.

doi:10.1006/eesa.2000.1960

Cited by 30 publications

(17 citation statements)

References 28 publications

(12 reference statements)

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“…Subcellular fractionation was obtained by the method of Kumar and Upretil [15]. In the stationary phase, the cells grown in presence of 1 mM cadmium were harvested by centrifugation (15,000 × g, 25 min, 4 • C).…”

Section: Subcellular Fractionationmentioning

confidence: 99%

Biosynthesis of cadmium sulfide nanoparticles by photosynthetic bacteria Rhodopseudomonas palustris

Bai

Zhang

Guo

et al. 2009

Colloids and Surfaces B: Biointerfaces

262

102

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Section: Subcellular Fractionationmentioning

confidence: 99%

Biosynthesis of cadmium sulfide nanoparticles by photosynthetic bacteria Rhodopseudomonas palustris

Bai

Zhang

Guo

et al. 2009

Colloids and Surfaces B: Biointerfaces

262

102

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“…Kumar & Upreti (2000) also showed that tbe presence of lead stimulated the excretion of carbohydrate by E. coli into the extracellular fiuid. According to Comte et al (2006), polysaccharides are most abundant component of soluble EPS, and their ability to bind metallic cafions could be involved in the great biosorption ability of soluble EPS.…”

Section: Effect Of Ce^ On Smp Compositionmentioning

confidence: 93%

Effect of Ce3+ on soluble microbial products production in anaerobic granular sludge digestion

Liang

Xia

et al. 2011

Water Science and Technology

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Upflow anaerobic sludge bed reactors fed on glucose were used to investigate the effects of Ce(3+) on soluble microbial products (SMP) production, which is the majority of the residual chemical oxygen demand present in the effluent. It was found that Ce(3+) concentration of 0.05 mg/L had no significant effect on the amount of SMP production, whereas that of 1 mg/L led to the increase in SMP production. The molecular-weight distribution and carbohydrate analysis indicated that an increase in SMP production may be partly attributed to the release of extracellular polymeric substances (EPS) into the bulk solution resulted from cerium toxicity, and the nucleic acids analysis suggested that increased cell lysis also contributed to SMP accumulation in the presence of Ce(3+). The increase in SMP production in the presence of Ce(3+) is possibly a consequence of the release of EPS and increased cell lysis due to cerium toxicity.

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“…The resulting supernatant containing 4 -5 mg of protein/ml was termed as cell extract. The subcellular fractionation was carried out as described by KUMAR and UPRETI (2000). Briefly, the cells were harvested by centrifugation (10,000 g, 15 min, 4 °C), washed twice with 30 mM Tris-buffer (pH 8.1) containing 2.5 mM EDTA, and re-suspended in the same buffer.…”

Section: Methodsmentioning

confidence: 99%

A novel membrane glycoprotein of Escherichia coli

Kumar

Mishra

Upreti

2003

J. Basic Microbiol.

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A novel glycoprotein (Gp45) has been isolated and purified from Escherichia coli. To our knowledge, Gp45 is the third glycoprotein isolated from E. coli membrane and it is the second in the non-pathogenic strain of the organism. For the isolation of Gp45, cell extract or membrane fraction was treated with sodium deoxycholate for 4 h and precipitated with trichloroacetic acid (TCA). The supernatant fraction of TCA containing the Gp45 was further purified on DEAE-Sephadex A-25. SDS-PAGE showed a single band at 45 kDa position that stained with periodic-Schiff reagent. It contained 60% carbohydrate and 40% protein content. The monosaccharide composition also substantiated the characteristics of the glycoprotein. The E. coli grown in presence of (14)C-glucosamine further confirmed the localization and biosynthesis of this glycoprotein on the membrane during the growth phase. Bacitracin, a general inhibitor of the glycosylation, inhibited its biosynthesis.

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Impact of Lead Stress and Adaptation in Escherichia coli

Cited by 30 publications

References 28 publications

Biosynthesis of cadmium sulfide nanoparticles by photosynthetic bacteria Rhodopseudomonas palustris

Biosynthesis of cadmium sulfide nanoparticles by photosynthetic bacteria Rhodopseudomonas palustris

Effect of Ce3+ on soluble microbial products production in anaerobic granular sludge digestion

A novel membrane glycoprotein of Escherichia coli

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