Biosynthesis of cadmium sulfide nanoparticles by photosynthetic bacteria Rhodopseudomonas palustris

Bai, Haotian; Zhang, Z.M.; Guo, Yanzhi; Guan-e, Yang

doi:10.1016/j.colsurfb.2008.12.025

Cited by 261 publications

(116 citation statements)

References 21 publications

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“…In addition, the purified smCSE enzyme, by itself, is capable of aqueous phase synthesis of CdS nanocrystals directly from cadmium acetate and L-cysteine. The resulting CdS nanocrystals are within the quantum confined size range and display optoelectronic properties analogous to those previously described for cell-based or chemically synthesized CdS nanocrystals (21-23, 32, 34-37 (33,(38)(39)(40)(41)(42) and suggested that H 2 S generation from CSE was the primary driver for CdS precipitation. To determine whether the putative CSE identified from S. maltophilia (smCSE) was capable of H 2 S generation from L-cysteine, smCSE was heterologously overexpressed and purified from Escherichia coli and the intrinsic kinetics of L-cysteine turnover to H 2 S measured (described in SI Appendix, SI Materials and Methods).…”

Section: Significancesupporting

confidence: 70%

Single-enzyme biomineralization of cadmium sulfide nanocrystals with controlled optical properties

Dunleavy

Kiely

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

115

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Nature has evolved several unique biomineralization strategies to direct the synthesis and growth of inorganic materials. These natural systems are complex, involving the interaction of multiple biomolecules to catalyze biomineralization and template growth. Herein we describe the first report to our knowledge of a single enzyme capable of both catalyzing mineralization in otherwise unreactive solution and of templating nanocrystal growth. A recombinant putative cystathionine γ-lyase (smCSE) mineralizes CdS from an aqueous cadmium acetate solution via reactive H 2 S generation from L-cysteine and controls nanocrystal growth within the quantum confined size range. The role of enzymatic nanocrystal templating is demonstrated by substituting reactive Na 2 S as the sulfur source. Whereas bulk CdS is formed in the absence of the enzyme or other capping agents, nanocrystal formation is observed when smCSE is present to control the growth. This dualfunction, single-enzyme, aerobic, and aqueous route to functional material synthesis demonstrates the powerful potential of engineered functional material biomineralization.cadmium sulfide | quantum dot | biomineralization | enzyme | nanoparticle B iological systems have evolved a diverse array of mechanisms to synthesize inorganic materials from aqueous solutions under ambient conditions. This inherent control over material properties has created interest in using these biological routes to synthesize materials (1-3) such as biosilica from sponges and diatoms (4-8), biogenic CaCO 3 from mollusks (9-12), and magnetic particles from magnetotactic bacteria (13-15). Designing a biomineralization strategy requires control of both the material composition and structure; in nature, this control is typically achieved through the assembly of a multiprotein complex, including both structuredirecting proteins and proteins responsible for mineralization of a specific composition. In the current work, we demonstrate the reduction of this complexity to its simplest form: a single enzyme capable of both catalyzing CdS mineralization and controlling particle size within the quantum confined size range to form functional biomineralized CdS quantum dots.Two of the most studied biomineralization proteins are perlucin and silicatein. Perlucin (16) has been shown to mineralize crystalline forms of CaCO 3 , a common structural material that constitutes the shell of many marine organisms, in the form of organic-inorganic composites. The role of the nacre protein perlucin in crystallite templating has been elucidated through experiments demonstrating crystallite formation in the presence of purified perlucin, and perlucin selectively being removed from solution during crystallite formation in the presence of a mixture of water-soluble, nacre-associated proteins (17). Native silicatein harvested from sea sponge or engineered forms produced recombinantly are active for biomineralization of silica and titania into structures that are amorphous or crystalline (7,18,19). In particular, biomineralizatio...

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Section: Significancesupporting

confidence: 70%

Single-enzyme biomineralization of cadmium sulfide nanocrystals with controlled optical properties

Dunleavy

Kiely

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

115

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“…In case of PbS nanoparticles synthesized using the immobilized bacteria, Rhodobacter sphaeroides it was reported that the nanoparticles were about 10.5 nm in size for cells harvested just after exponential growth phase (after 32 h of growth). 34 An increase in size of the nanoparticles to 43.2 nm was observed in the stationary phase cells (after 40 h of growth). The growth in size was attributed to nucleation effect wherein small particles aggregated to form larger grains.…”

Section: Resultsmentioning

confidence: 93%

Green synthesis of lead sulfide nanoparticles by the lead resistant marine yeast, Rhodosporidium diobovatum

Seshadri

Saranya

Kowshik

2011

Biotechnology Progress

175

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Biosynthesis of nanoparticles using microorganisms has attracted a lot of attention in recent years as this route has the potential to lead to synthesis of monodisperse nanoparticles. Here, we report the intracellular synthesis of stable lead sulfide nanoparticles by a marine yeast, Rhodosporidium diobovatum. The PbS nanoparticles were characterized by UV-visible absorption spectroscopy, X-ray diffraction (XRD) and energy dispersive atomic spectroscopy (EDAX). UV-visible absorption scan revealed a peak at 320 nm, a characteristic of the nanosize range. XRD confirmed the presence of PbS nanoparticles of cubic structure. Crystallite size as determined from transmission electron microscopy was found to be in the range of 2-5 nm. Elemental analysis by EDAX revealed the presence of particles composed of lead and sulfur in a 1:2 ratio indicating that PbS nanoparticles were capped by a sulfur-rich peptide. A quantitative study of lead uptake through atomic absorption spectrometry revealed that 55% of lead in the medium was accumulated in the exponential phase, whereas a further 35% was accumulated in the stationary phase; thus, the overall recovery of PbS nanoparticles was 90%. The lead-exposed yeast displayed a marked increase (280% over the control) in nonprotein thiols in the stationary phase.

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“…The content of C-S lyase in R.palustris was suggested to be responsible for nanocrystal formation. C-S lyase is found to be an intracellular enzyme located in the cytoplasm and it was indicated that R.palustris synthesized CdS nanoparticles intracellularly, later discharging it (Bai et al, 2009).…”

Section: Some Of the Mechanistic Aspects Of Nanoparticle Formationmentioning

confidence: 99%

Biomimetic Synthesis of Nanoparticles: Science, Technology & Applicability

Prathna¹,

Mathew²,

Chandrasekaran³

et al. 2010

Biomimetics Learning From Nature

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Biosynthesis of cadmium sulfide nanoparticles by photosynthetic bacteria Rhodopseudomonas palustris

Cited by 261 publications

References 21 publications

Single-enzyme biomineralization of cadmium sulfide nanocrystals with controlled optical properties

Single-enzyme biomineralization of cadmium sulfide nanocrystals with controlled optical properties

Green synthesis of lead sulfide nanoparticles by the lead resistant marine yeast, Rhodosporidium diobovatum

Biomimetic Synthesis of Nanoparticles: Science, Technology & Applicability

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Biosynthesis of cadmium sulfide nanoparticles by photosynthetic bacteria Rhodopseudomonas palustris

Cited by 261 publications

References 21 publications

Single-enzyme biomineralization of cadmium sulfide nanocrystals with controlled optical properties

Single-enzyme biomineralization of cadmium sulfide nanocrystals with controlled optical properties

Green synthesis of lead sulfide nanoparticles by the lead resistant marine yeast, Rhodosporidium diobovatum

Biomimetic Synthesis of Nanoparticles: Science, Technology &amp; Applicability

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Product

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Biomimetic Synthesis of Nanoparticles: Science, Technology & Applicability