Green synthesis of lead sulfide nanoparticles by the lead resistant marine yeast, Rhodosporidium diobovatum

Abstract

Biosynthesis of nanoparticles using microorganisms has attracted a lot of attention in recent years as this route has the potential to lead to synthesis of monodisperse nanoparticles. Here, we report the intracellular synthesis of stable lead sulfide nanoparticles by a marine yeast, Rhodosporidium diobovatum. The PbS nanoparticles were characterized by UV-visible absorption spectroscopy, X-ray diffraction (XRD) and energy dispersive atomic spectroscopy (EDAX). UV-visible absorption scan revealed a peak at 320 nm, a characteristic of the nanosize range. XRD confirmed the presence of PbS nanoparticles of cubic structure. Crystallite size as determined from transmission electron microscopy was found to be in the range of 2-5 nm. Elemental analysis by EDAX revealed the presence of particles composed of lead and sulfur in a 1:2 ratio indicating that PbS nanoparticles were capped by a sulfur-rich peptide. A quantitative study of lead uptake through atomic absorption spectrometry revealed that 55% of lead in the medium was accumulated in the exponential phase, whereas a further 35% was accumulated in the stationary phase; thus, the overall recovery of PbS nanoparticles was 90%. The lead-exposed yeast displayed a marked increase (280% over the control) in nonprotein thiols in the stationary phase.

Biogenic Synthesis of Nanoparticles and Potential Applications: An Eco- Friendly Approach

“…The silver and gold nanoparticles biosynthesis was also investigated by Mourato et al [50], using an extremophilic yeast strain isolated from acid mine drainage. The marine yeast Rhodosporidium diobovatum has been explored for intracellular synthesis of stable lead sulfide nanoparticles [51]. like viruses, proteins, peptides and enzymes could be exploited for biosynthesis of nanoparticles (Figure 3).…”

Carbohydrate components of culture media as determinants of the Rhodosporidium diobovatum IMB Y-5023 yeast metabolism

“…It can grow not only on various media, but also under various environmental conditions. Its wild strains are found in the depths of the sea (Seshadri et al, 2011), in soil and on the surface of plants i.e. in various envir- Ielchishcheva, Iu., Stachowiak, B., Szwengiel, A., Bozhkov, A.…”

A Study on Extracellular Synthesis of Silver Nanoparticles from Endophytic Fungi, Isolated from Ethanomedicinal Plants <i>Curcuma longa</i> and <i>Catharanthus roseus</i>

“…Synthesis of AgNps by biological route is easy conventional method compared with chemical, physical, and photochemical methods. As these methods are capital intensive, toxic and non ecofriendly, a biological synthesis of AgNps with the help of plants and microorganisms including bacteria, actinomycetes, algae, and fungi, which has been realized globally due to its ecofriendly, cost effective and biocompatible synthesis, which has made the researchers to exploit the biological sources as nanofactories [5,6,7]. Fungal mediated synthesis of AgNps is determined nowadays because of their reception towards higher bioaccumulation, easy and simple scale up method, economic viability, compared to other synthesis procedures from bacteria, actinomycetes, algae, and plants.…”