Alcohol dehydrogenase gene expression in potato following elicitor and stress treatment

Abstract

A cDNA clone corresponding to a mRNA that rapidly accumulates during the hypersensitive-like response induced by elicitor treatment of potato (Solanum tuberosum L.) tuber was characterized. The clone encodes a polypeptide (Mr = 41,097) having 83%-85% amino acid identity with known plant alcohol dehydrogenase sequences (ADH; EC 1.1.1.1). The identity of the clone was confirmed by measuring the ADH enzyme activity in extracts of Escherichia coli transformed with the cDNA clone. In potato tuber disks, a wide range of stresses, including treatment with fatty acid elicitors, salicylic acid, UV light and anaerobiosis, was shown to induce accumulation of Adh transcripts. In stems, a high constitutive level of Adh transcripts could be detected in 4-week old plants, but not in 8-week old plants. However, the mRNA could be induced to accumulate in stems of 8-week old plants by treatment with arachidonic acid elicitor or by anaerobiosis. Induction in leaves was also obtained during anaerobiosis and after treatment with a Phytophthora infestans mycelial homogenate.

Bioenergetics of Monoterpenoid Essential Oil Biosynthesis in Nonphotosynthetic Glandular Trichomes

“…Given that monoterpenol dehydrogenases are among the most highly expressed genes in secretory phase GTs (Lange et al, 2000;Ahkami et al, 2015), one may postulate that the ADH activity we observed is a nonspecific side activity of these enzymes. To test this hypotheses, we first performed a BLASTx search with the wellcharacterized ADH1 sequence from potato (Solanum tuberosum; Matton et al, 1990) against the peppermint GT transcriptome data set. This search indicated the presence of two putative orthologs (at roughly 88% identity for the translated peptide sequence) that were expressed at high levels in peppermint GTs (accession nos.…”

“…The sequence of the well-characterized ADH1 from potato (Solanum tuberosum; P14673; Matton et al, 1990) was used to query our peppermint (Mentha 3 piperita) transcriptome assembly. Two contigs that contained genes with high identity (;88% at the peptide sequence level) and the corresponding cDNAs were amplified using Phusion DNA polymerase (New England Biolabs) with an annealing temperature of 60°C.…”

Identification of Differentially Expressed Genes by cDNA-AFLP Technique in Response to Drought Stress in Triticum durum

“…ADH activity is considered essential for the survival of plants during anaerobic conditions (66). Transcriptional activation of the Adh gene has also been noticed in response to several environmental stresses including saline conditions (67,68). In our experiment, ADH was induced under drought stress treatment.…”

The Effects of Calcium Nutrition on the Activities of Lactate Dehydrogenase, Alcohol Dehydrogenase and Other Enzymes in Melon (Cucumis melo L.) Seedlings Subjected to Flooding

“…침수 상태에서 합 성이 증가되는 단백질 중의 하나인 알코올 가수분해효소 (ADH: Alcohol dehydrogenase, EC 1.1.1.1)는 혐기적 조건 에서 식물이 적응하거나 생명을 유지하는데 필수적이며, 이 효소의 유전자 (Adh)의 전사는 침수 또는 산소 부족에 대한 특징적인 반응이 될 수 있다고 알려져 있다 (Sach et al, 1980;Johnson et al, 1994). 알코올 가수분해효소 활성도 변화는 토마토 (Genez et al, 1993), 아라비돕시스 (Chung and Ferl, 1999), 귀리 (Biemelt et al, 1998), 보리 (Mayne and Lea, 1984), 옥수수 (Andrew et al, 1994), 벼 (Ismail et al, 2009), 두과작물 (Fukuda et al, 2005), 감자 (Matton et al, 1990) (Sedbrook et al, 1996;Chung and Ferl, 1999;Peng et al, 2001). 식 물체가 생육하고 있는 상태에서 산소가 부족해지면 세포질 내 칼슘의 농도가 빠르게 증가하는 경향이 있으며 이러한 조건에서는 액포 등에 저장된 칼슘이 세포질로 유리되는 것 으로 추정하고 있다 (Subbaiah et al, 1998 (Subbaiah et al, 1994;Bush, 1996;Sedbrook et al, 1996).…”