Albumin Conjugating Amino Acid Schiff-Base Metal Complexes for Scavenging Superoxide Anion Radical

Zhou, Xu; Pan, Sujuan; Li, Gang; He, Yufeng; Wang, Rong‐Min

doi:10.1007/s10904-015-0242-1

Cited by 7 publications

(5 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…S5(b)] suggesting the conjugation of CoMSPs with BSA. This is supported by the observation of Xu et al that BSA after conjugation with cobalt shows an absorbance within 300-500 nm wavelength region and it is often weakly conjugated with cobalt atom [17]. Also, while adding DA to this BSA conjugated CoMSPs, a Fig.…”

Section: Colorimetric Sensing Of Dopaminesupporting

confidence: 73%

“…Bovine serum albumin (BSA) is a bio-ligand that is capable of stabilizing and dispersing metal hydroxides [15,16]. Xu et al used BSA to conjugate cobalt based Schiff base complexes and demonstrated them as a potential superoxide anion radical scavenger [17]. This study shows the versatility of BSA towards cobalt binding ability.…”

Section: Introductionmentioning

confidence: 80%

“…Further each of these domains is subdivided into two sub domains namely A and B. BSA often involves in complexation with metals through its α-helical structure. Based on the previous report by Xu et al on the analysis of binding phenomenon between cobalt based metal complexes and BSA, the most probable binding site is identified to be "N" atom of α helical structure in BSA [17,28].…”

Section: Elucidation Of Sensing Mechanismmentioning

confidence: 99%

See 2 more Smart Citations

Preparation of topographically remarkable cobalt microspheres for colorimetric detection of dopamine and elucidation of its sensing mechanism

Mers

Ganesh

2019

SN Appl. Sci.

View full text Add to dashboard Cite

In this work, a new colorimetric probe is prepared and demonstrated for the detection of dopamine (DA), an important neurotransmitter. The proposed colorimetric probe is prepared using bovine serum albumin (BSA) modified cobalt hydroxide microspheres (CoMSPs@BSA) and subsequently explored for the selective colorimetric detection of DA. The sensing studies are monitored using UV-visible absorbance spectroscopy. Interestingly upon addition of DA, an intense blue color is formed suggesting the potential use of this probe for naked eye detection of DA. The mechanism behind the sensing is investigated using time dependent UV-visible absorbance spectroscopic study and cyclic voltammetry. These studies clearly indicate the ligand exchange reaction is responsible for the resultant blue color formation. Further using this colorimetric probe a concentration range from 5.3 to 211 µM DA could easily be detected. Limit of detection of this DA sensor is found to be 5 × 10 −6 M. Similarly a linear relation is observed from the calibration graph plotted between the concentration and absorption intensity corresponding to ligand exchange of BSA by DA. Moreover, this sensing probe (CoMSPs@BSA) is found to be highly selective among a wide range of other commonly interfering biomolecules.Graphic abstract Topographically remarkable cobalt microspheres are prepared using a simple method and explored for the colorimetric detection of dopamine. The sensing mechanism is elucidated using UV-visible spectroscopic and electrochemical studies.

show abstract

Section: Colorimetric Sensing Of Dopaminesupporting

confidence: 73%

Section: Introductionmentioning

confidence: 80%

Section: Elucidation Of Sensing Mechanismmentioning

confidence: 99%

See 1 more Smart Citation

Preparation of topographically remarkable cobalt microspheres for colorimetric detection of dopamine and elucidation of its sensing mechanism

Mers

Ganesh

2019

SN Appl. Sci.

View full text Add to dashboard Cite

show abstract

“…The EC 50 of BSA-Cu is 0.038±0.001 μmol L −1 , which is the lowest of all. The EC 50 of natural SOD 35 is 0.041± 0.001 μmol L −1 . The EC 50 of BSA-Cu reaches 107% compared with natural Cu, Zn-SOD.…”

Section: Superoxide Anion Radical (O •−mentioning

confidence: 96%

Bovine Serum Albumin Metal Complexes for Mimic of SOD

Song

et al. 2016

J Chem Sci

Self Cite

View full text Add to dashboard Cite

Superoxide anion radical (O •− 2) is a noxious reactive oxygen species (ROS). Transition metal ion complexes have been generally used as antioxidants to eliminate ROS. In this work, a neoteric watersoluble biopolymer metal complex (BSA-M) was prepared by conjugating the soluble biopolymer bovine serum albumin (BSA) with three transition metal ions (M, M=Cu, Co, Mn). The binding mode and ratio of metal ions bound to albumin were investigated. The BSA-M complexes were characterized by UV-Vis, circular dichroism (CD) spectra and polyacrylamide gel electrophoresis (PAGE). BSA served as polymer scaffold and the metal complex functioned as the catalytic active center. The results demonstrated that the structure of BSA remained unchanged when the binding ratio of transition metal ion complex to BSA was 5:1. Furthermore, the scavenging superoxide anion free radical (O •− 2) activity of biopolymer-metal complexes were determined by nitroblue tetrazolium light reduction assay method. The antioxidant capacity of BSA-M has markedly increased. The conjugated BSA-M (M=Cu, Mn) showed preeminent scavenging activity for O •− 2 , and the EC 50 value of the BSA-Cu was 0.038±0.0013 μmol•L −1 , which is comparable to EC 50 value (0.041±0.001 μmol•L −1) of the natural superoxide dismutase (SOD), the analog quantity reached 107%. As a consequence, it can be considered as a bio-functional mimic of enzyme SOD and has a promising application prospect in antioxidant drug field.

show abstract

“…We hypothesized that RCA 120 could bind 4a, as reported for other galactose conjugates [ 35 , 53 ], and that the protein environment could modulate the activity of the catalytic center, as found for other SOD-mimetic hybrid systems [ 39 , 54 , 55 , 56 ]. The interaction between the RCA 120 and 4a complex was investigated using CD spectroscopy.…”

Section: Resultsmentioning

confidence: 97%

New Glycosalen–Manganese(III) Complexes and RCA120 Hybrid Systems as Superoxide Dismutase/Catalase Mimetics

Lanza,

Vecchio

2023

Biomimetics

View full text Add to dashboard Cite

Reactive oxygen species are implicated in several human diseases, including neurodegenerative disorders, cardiovascular dysfunction, inflammation, hereditary diseases, and ageing. MnIII–salen complexes are superoxide dismutase (SOD) and catalase (CAT) mimetics, which have shown beneficial effects in various models for oxidative stress. These properties make them well-suited as potential therapeutic agents for oxidative stress diseases. Here, we report the synthesis of the novel glycoconjugates of salen complex, EUK-108, with glucose and galactose. We found that the complexes showed a SOD-like activity higher than EUK-108, as well as peroxidase and catalase activities. We also investigated the conjugate activities in the presence of Ricinus communis agglutinin (RCA120) lectin. The hybrid protein–galactose–EUK-108 system showed an increased SOD-like activity similar to the native SOD1.

show abstract

Albumin Conjugating Amino Acid Schiff-Base Metal Complexes for Scavenging Superoxide Anion Radical

Cited by 7 publications

References 25 publications

Preparation of topographically remarkable cobalt microspheres for colorimetric detection of dopamine and elucidation of its sensing mechanism

Preparation of topographically remarkable cobalt microspheres for colorimetric detection of dopamine and elucidation of its sensing mechanism

Bovine Serum Albumin Metal Complexes for Mimic of SOD

New Glycosalen–Manganese(III) Complexes and RCA120 Hybrid Systems as Superoxide Dismutase/Catalase Mimetics

Contact Info

Product

Resources

About