Bovine Serum Albumin Metal Complexes for Mimic of SOD

He, Yufeng; Li, Gang; Song, Pengfei; Wang, Rong‐Min

doi:10.1007/s12039-016-1184-z

Cited by 14 publications

(7 citation statements)

References 37 publications

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“…The results indicated that the mount of metal ions (Cu II ) had important effect for anti-O Table 6 and Figure 10. Compared with the literature [31], of which EC 50 and degree of simulation, respectively, were 0.038 ± 0.003 mol/L and 107%; here EC 50 of prepared FK-GlyCu 60 was far below it and the degree of simulation was much bigger than it. The values, respectively, were up to 0.0045 ± 0.0012 mol/L and 911.1%.…”

Section: Ocontrasting

confidence: 73%

Preparation of Keratin-Glycine Metal Complexes and Their Scavenging Activity for Superoxide Anion Radicals

Wang

et al. 2018

International Journal of Polymer Science

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To address the problem of limited application of natural SOD, the development of SOD mimic enzymes is of great importance for bioantioxidation. Herein, we report on a new type of biopolymer antioxidant with excellent scavenging activity for O2•-, keratin-glycine metal complexes (FK-GlyM, M = Zn, Cu, Mn, Ni). They are prepared by feather keratin firstly combined with glycine and then metal ions. Using FT-IR, TG, CD, and SEM, the performance of the obtained complexes (FK-GlyM) for scavenging O2•- is analysed and investigated. Importantly, the scavenging activity of FK-GlyCu is excellent in all FK-GlyM, and FK-GlyCu60 has the most excellent anti-O2•- activity in all FK-GlyCux, of which EC50 and degree of simulation were, respectively, up to 4.5×10-3±0.0012 μmol/L and 911.1% compared with nature Cu, Zn-SOD. Finally, its mechanism was also discussed. In summary, this method about the simulation strategies will provide a novel idea for exploiting new-type biocompatible and highly reactive antioxidants.

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Section: Ocontrasting

confidence: 73%

Preparation of Keratin-Glycine Metal Complexes and Their Scavenging Activity for Superoxide Anion Radicals

Wang

et al. 2018

International Journal of Polymer Science

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“…Alkaline DMSO, a source of superoxide radical (O -2 ) and nitrobluetetrazolium (NBT) as a scavenger (O -2 ) were used to test in vitro SOD activity [44]. In general, a solution containing 2.1 mL of 0.2 M potassium phosphate buffer (pH 8.6) and 1 mL of 56 mM NBT was applied to the 400 mL sample to be analyzed.…”

Section: Sod Activitymentioning

confidence: 99%

In vitro and in vivo biochemical activities of novel copper complexes of chrysin derivatives of organosulfur compounds based potential therapeutic agent: design, synthesis, characterization and pharmacological studies

Ebenezer

Nagashri

2022

Preprint

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Novel series of chrysin compound containing the organosulfur moiety from the Schiff bases derived from chrysin and 2-(phenylthio)aniline and their copper complexes were synthesized. They were characterized using analytical and spectral techniques. All the Cu(II) complexes exhibit the distortion square planar geometry. The in vitro antimicrobial activities of the investigated compounds were tested against the bacterial species, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, and Klebsiella pneumoniae and fungal species, Aspergillus niger, Fusarium solani, Rhizoctonia bataicola and Candida albicans by disc diffusion process. Free radical scavenging, superoxide dismutase, glutathione peroxidase and antioxidant activities of the copper complexes have also been studied. The DNA-cleavage activities of Cu(II) complexes, have been investigated by using agarose gel electrophoresis. Furthermore, interaction of copper complexes with bovine serum albumin was investigated by fluorescence spectrometry. The anticancer activities of the complexes have been carried out towards HeLa and HCT116 cancer cells. The pretreatment of chrysin compounds containing the organosulfur moiety of Cu(II) complexes ([CuHL4l(OAc)], [CuHL4m(OAc)] and [CuHL4n(OAc)]) elicited marked anti-inflammtory effects in carrageenan induced paw edema test in mice during various assessment times (1–5 hrs).

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“…Penentuan nilai IC 50 , yang merupakan aktivitas penangkal radikal superoksida dari sampel kompleks, didapatkan dengan menarik garis dari inhibisi 50% pada plot kurva antara persen inhibisi dan konsentrasi kompleks (Deawati et al 2017;Yan et al 2016).…”

Section: Persamaanunclassified

Metode Non-Enzimatik Riboflavin-Nitrobluetetrazolium (Rb-NBT) Sebagai Teknik Penentuan Aktivitas Penangkal Radikal Bebas Anion Superoksida pada Kompleks Mangan(III)-Salen

et al. 2018

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Abstrak: Pada penelitian ini dilakukan pengujian aktivitas penangkal radikal bebas superoksida secara in vitro terhadap senyawa kompleks polimerik [Mn(salen)N(CN) 2 ] n , baik dengan metode non-enzimatik maupun enzimatik. Aktivitas penangkal radikal bebas superoksida yang didapatkan dari kedua metode ini dinyatakan dengan IC 50 . Melalui metode non-enzimatik riboflavin-nitrobluetetrazolium (Rb-NBT), radikal superoksida yang dihasilkan dari penyinaran riboflavin, bereaksi dengan nitrobluetetrazolium sebagai indikator dan senyawa kompleks, lalu serapan NBT tereduksi diukur pada panjang gelombang 560 nm. Dengan metode non-enzimatik ini, dihasilkan nilai IC 50 yang tinggi, konsisten, dan standar deviasinya sangat kecil, yaitu 3,4 ± 0,4 µM. Sedangkan secara enzimatik, radikal superoksida yang dihasilkan dari reaksi antara xantin dan xantin oksidase ditangkap oleh sitokrom C (sebagai indikator) dan senyawa kompleks, kemudian sitokrom C tereduksi diukur serapannya pada panjang gelombang 450 nm. Melalui metode enzimatik McCord-Fridovich (McCF) ini, dihasilkan beberapa nilai IC 50 kompleks [Mn(salen)N(CN) 2 ] n yang tidak konsisten, yang disebabkan oleh tidak berfungsinya enzim. Dengan demikian, metode non-enzimatik Rb-NBT lebih mudah dan lebih akurat untuk digunakan sebagai teknik penentuan aktivitas penangkal radikal anion superoksida dibandingkan dengan metode enzimatik.Kata kunci: penangkal radikal bebas, superoksida, non-enzimatik, [Mn(III)(salen)N(CN) 2 ], riboflavin-NBT Abstract: In this research, the in vitro activity test of superoxide free-radical scavenger has been done to the polymeric complex compound of [Mn(salen)N(CN) 2 ], either by non-enzymatic or enzymatic method. The activity of superoxide free radical scavenger obtained from these two methods expressed by IC 50 . Through the nonenzymatic method of riboflavin-nitrobluetetrazolium (Rb-NBT), superoxide radicals generated by riboflavin irradiation, was reacted with nitrobluetetrazolium as an indicator and complex compound, then the reduced NBT absorbances were measured at a wavelength of 560 nm. With the non-enzymatic method, resulted the IC 50 value that are high, consistent, and its standard deviation was very small, i.e. 3.4 ± 0.4 µM. While enzymatically, superoxide radicals generated from the reaction between xanthine and xanthine oxidase were captured by the cytochrome C (as an indicator) and complex compound, then the reduced cytochrome C absorbances were measured at a wavelength of 450 nm. Through the McCord-Fridovich (McCF) enzymatic method, produced some of IC 50 values of [Mn(salen)N(CN) 2 ] n complex which inconsistent, caseud by unfunctional of enzyme. Thus, the non-enzymatic method Rb-NBT is more simple and accurate to be used as a technique of determining the activity of anion superoxide radical scavenger than the enzymatic method.

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Bovine Serum Albumin Metal Complexes for Mimic of SOD

Cited by 14 publications

References 37 publications

Preparation of Keratin-Glycine Metal Complexes and Their Scavenging Activity for Superoxide Anion Radicals

Preparation of Keratin-Glycine Metal Complexes and Their Scavenging Activity for Superoxide Anion Radicals

In vitro and in vivo biochemical activities of novel copper complexes of chrysin derivatives of organosulfur compounds based potential therapeutic agent: design, synthesis, characterization and pharmacological studies

Metode Non-Enzimatik Riboflavin-Nitrobluetetrazolium (Rb-NBT) Sebagai Teknik Penentuan Aktivitas Penangkal Radikal Bebas Anion Superoksida pada Kompleks Mangan(III)-Salen

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