Albumin binds self-assembling dyes as specific polymolecular ligands

Stopa, Barbara; Rybarska, Janina; Drozd, Anna; Konieczny, Leszek; Król, Marcin; Lisowski, Marek; Piekarska, Barbara; Roterman, Irena; Spólnik, Paweł; Zemanek, Grzegorz

doi:10.1016/j.ijbiomac.2006.05.002

Cited by 28 publications

(31 citation statements)

References 44 publications

(69 reference statements)

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“…However, albumin has a propensity to bind a wide variety of organic, inorganic, negatively, and positively charged ligands. The binding affinities of albumin for different ligands or peptides are often as high as those of the specific interactions (26,43). Therefore, in initial experiments, we compared the influences of albumin-containing and nonalbumin-containing vehicle solution on the function of lymphatics.…”

Section: Discussionmentioning

confidence: 99%

Inhibition of myosin light chain phosphorylation decreases rat mesenteric lymphatic contractile activity

Wang¹,

Nepiyushchikh²,

Zawieja³

et al. 2009

American Journal of Physiology-Heart and Circulatory Physiology

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Based on the well-established finding that the phosphorylation of myosin light chain 20 (MLC20) plays an essential role in blood vessel smooth muscle contraction, we investigated if phosphorylated MLC20 (pMLC20) would modulate the tonic and/or phasic contractions of lymphatic muscle. The effects of ML-7, a MLC kinase inhibitor (1-10 M), were tested on the contractile parameters of isolated and cannulated rat mesenteric lymphatics during their responses to the known modulators, pressure (1-5 cmH2O) and substance P (SP; 10 Ϫ7 M). Immunohistochemical and Western blot analyses of pMLC20 were also performed on isolated lymphatics. The results showed that 1) increasing pressure decreased both the lymphatic tonic contraction strength and pMLC20-to-MLC20 ratio; 2) SP increased both the tonic contraction strength and phosphorylation of MLC20; 3) ML-7 decreased both the lymphatic tonic contraction strength and pMLC20-to-MLC20 ratio; and 4) the increase in lymphatic phasic contraction frequency in response to increasing pressure was diminished by ML-7; however, the phasic contraction amplitude was not significantly altered by ML-7 either in the absence or presence of SP. These data provide the first evidence that tonic contraction strength and phasic contraction amplitude of the lymphatics can be differentially regulated, whereby the increase in MLC20 phosphorylation produces an activation in the tonic contraction without significant changes in the phasic contraction amplitude. Thus, tonic contraction of rat mesenteric lymphatics appears to be MLC kinase dependent. ML-7; lymphatic contraction; tonic contraction; phasic contraction THE LYMPHATIC SYSTEM plays essential roles by returning the protein-rich interstitial fluid to the blood circulation for fluid homeostasis, transporting lipids and lipid-soluble vitamins absorbed from the intestines to the circulation for nutrition, and distributing immune cells to the lymph nodes for defense against diseases. All of these vital functions rely on the contractile activities of the muscle cells residing in the lymphatic vessel wall. The muscular lymphatic vessels are composed of many basic structural and functional units called lymphangions, which are capable of exhibiting intrinsic contractile activities to propel lymph flow through the network of the lymphatic vessels. Although the classical influence of pressure and flow on lymphatic contractility has been well documented (14 -16, 24, 31, 37, 38, 51), the molecular mechanisms regulating the contractile activities of the lymphatic muscle are not well understood.Cardiac muscles and most vascular smooth muscles (VSMs) exercise their physiological functions by phasic and tonic contractions, respectively, whereas lymphatic muscle accomplishes its functions using both tonic and phasic contractions. In general, an increase in the intracellular Ca 2ϩ concentration ([Ca 2ϩ ] i ) is the primary mechanism that initiates muscle contraction, a mechanism shared by striated muscles and VSMs. In striated muscle, Ca 2ϩ binding to troponin C in...

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Section: Discussionmentioning

confidence: 99%

Inhibition of myosin light chain phosphorylation decreases rat mesenteric lymphatic contractile activity

Wang¹,

Nepiyushchikh²,

Zawieja³

et al. 2009

American Journal of Physiology-Heart and Circulatory Physiology

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“…Moreover, albumin can bind not only several single molecules of dye, but also self-assembling dyes such as Congo red or Evans blue. It is likely that specific polymolecular ligands penetrate the central crevice of albumin [11].…”

Section: Introductionmentioning

confidence: 99%

Interaction of serum albumin with vinyl sulfonate azo dye

Sereikaitė

Bumelis

2008

Open Chemistry

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“…Owing to its function, albumin is capable of binding various anionic compounds, of which the dye is an example. CR is furthermore capable of associating with amyloidogenic apolipoproteins [22][23][24][25][26].…”

Section: Natural Susceptibility Of Proteins To Bind Congo Redmentioning

confidence: 99%

Protein Conditioning for Binding Congo Red and Other Supramolecular Ligands

Zemanek

Jagusiak

Rybarska

et al. 2017

Self-Assembled Molecules – New Kind of Protein Ligands

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Self-assembled organic compounds which form ribbon-like micellar clusters may attach themselves to proteins, penetrating in areas of low stability. Such complexation involves regions other than the protein's natural binding site. The supramolecular ligand adheres to beta folds or random coils which become susceptible to complexation as a result of function-related structural changes -e.g. antibodies engaged in immune complexes or acute phase proteins. However, even seemingly unsusceptible helical proteins may bind Congo red if they include chameleon sequences (short peptide fragments capable of adopting different secondary conformations depending on environmental conditions). Examples of such proteins include hemoglobin and albumin. Complexation of supramolecular Congo red is often associated with increased fluorescence, indicating breakdown of ligand micelles in the complex. This phenomenon may be used in diagnostic tests. Keywords

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Albumin binds self-assembling dyes as specific polymolecular ligands

Cited by 28 publications

References 44 publications

Inhibition of myosin light chain phosphorylation decreases rat mesenteric lymphatic contractile activity

Inhibition of myosin light chain phosphorylation decreases rat mesenteric lymphatic contractile activity

Interaction of serum albumin with vinyl sulfonate azo dye

Protein Conditioning for Binding Congo Red and Other Supramolecular Ligands

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