AKTIVITAS SITOTOKSIK EKSTRAK SELADA MERAH (Lactuca sativa var. Crispa) PADA BERBAGAI PELARUT EKSTRAKSI

Rohmah, Jamilatur; Rini, Chylen Setiyo; Wulandari, Fitria

doi:10.20473/jkr.v4i1.13066

Cited by 16 publications

(29 citation statements)

References 7 publications

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“…K2a and K3c each produced Rf values of 0.7 cm and Rf 0.85 cm, which were thought to contain secondary flavonoid metabolites. According to a study conducted by Rohmah et al (2019), the resulting Rf values for flavonoid compounds were 0.88 cm and 0.83 cm, while Yuhernita & Juniarti (2011) stated that the Rf value of 0.707 was a flavonoid with a greenishbrown color. Then, K2b produced an Rf value of 0.8 cm, which was thought to contain secondary metabolites of steroids.…”

Section: Resultsmentioning

confidence: 99%

Antibacterial activity test on ethanol extract fraction of Kirinyuh (Chromolaena odorata L.) leaves for multi-drug resistant organisms bacteria

Gultom

Hartanti

Maritsa

et al. 2021

bio

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The resistance of pathogenic bacteria to antibiotics is increasing due to antibiotics with incorrect doses, wrong diagnostics, and the wrong target. Bacteria that have been resistant to several antibiotics are called multi-drug resistant organisms (MDRO) bacteria. Bacterial resistance to some antibiotics requires alternative herbal treatments, one of which is the Chromolaena odorata L. Research must therefore be conducted on the antibacterial activity of the ethanol extract fraction of C. odorata L. leaves for MDRO bacteria, such as Staphylococcus lugdunensis methicillin-resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa extended-spectrum beta-lactamase (ESBL), and Klebsiella pneumoniae ESBL. This study aims to determine the antibacterial activity of the ethanol extract of Kirinyuh leaves (C. odorata L.) and the antibacterial activity of the fractionation against MDRO bacteria. Test results of kirinyuh leaf ethanol extract for S. lugdunensis MRSA, P. aeruginosa ESBL, and K. pneumoniae ESBL bacteria each resulted in an inhibition zone with an average diameter of 11.6 mm (strong), 11.5 mm (strong), and 11.13 mm (strong), respectively. Testing the antibacterial activity of the ethanol fraction against MDRO bacteria can show antibacterial activity against all tested bacteria, namely Fraction 5. The results of the antibacterial activity of fraction 5 against K. pneumoniae ESBL, P. aeruginosa ESBL, and S. lugdunensis MRSA bacteria with the formation of inhibition zones formed of 10.2 mm (strong), 8.8 mm (moderate), and 7.9 mm (moderate), respectively. The results of thin-layer chromatography showed that the secondary metabolites contained in the fifth fraction were terpenoids, steroids, and flavonoids.

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Section: Resultsmentioning

confidence: 99%

Antibacterial activity test on ethanol extract fraction of Kirinyuh (Chromolaena odorata L.) leaves for multi-drug resistant organisms bacteria

Gultom

Hartanti

Maritsa

et al. 2021

bio

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“…These compounds have toxic properties that have the ability to kill Artemia salina larvae which work as stomach poisons (Rohmah et al, 2019).…”

Section: Toxicitymentioning

confidence: 99%

The Effect Of Mangrove Leaf Extract Dosage Sonneratia Alba On Hela Cell Viability

Suryaningrum

2021

JSCRTE

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Cervical cancer is caused due to infection from the Human Papilloma Virus (HPV) which attacks the sexually active female reproductive organs. Treatment is carried out alternatively using natural ingredients such as mangroves. Sonneratia alba is a type of mangrove plant that has been used in alternative medicine because of its potential as an anticancer. This study aims to determine the effect of Sonneratia alba mangrove extract on heLa cell viability. The Sonneratia alba leaf powder was extracted using stratified maceration. The solvents used include n-hexane, ethyl acetate, and ethanol. The results showed that the LC50 value was 3.59 ppm, this means that the ethyl acetate extract has toxic properties. Phytochemical test results of Sonneratia alba leaf extract contain alkaloid compounds, steroids / triterpenoids, and tannins. The results of the test yield extract were 12.60%, extract water content was 21.24%, and total phenol was 504.08 mg / g GAE Test The results of the LC-MS test resulted in the suspicion of compounds including diosmetin, caffeine, and turmeron. The ethyl acetate extract of Sonneratia alba leaves was cytotoxic against heLa cell viability with the resulting IC50 value of 478.630 µg / mL

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“…Sedangkan hasil uji fitokimia pada fraksi menunjukkan hasil yang sesuai dengan penelitian yang dilakukan oleh Rohmah et al, (2019) yang menyatakan fraksi nheksana mengandung tanin, alkaloid dan saponin, fraksi etil asetat mengandung tanin, flavonoid, alkaloid dan saponin, fraksi air mengandung tanin, flavonoid dan saponin.…”

Section: Hasil Uji Fitokimia Ekstrak Dan Fraksi Buah Karondaunclassified

SKRINING TOKSISITAS AKUT BEBERAPA FRAKSI BUAH KARONDA (Carissa carandas L.) PADA EMBRIO ZEBRAFISH (Danio rerio)

Rusli

Sari²,

Wardatun³

et al. 2020

fitofarmaka JIF

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Dalam pengembangan obat baru, perlu dilakukan penelitian untuk menentukan keamanan dan efek dari suatu tanaman obat. Buah karonda (Carissa carandas L.) adalah tanaman yang memiliki potensi sebagai obat anti-inflamasi. Penelitian ini bertujuan untuk menentukan toksisitas akut fraksi n-heksana, etil asetat, dan air buah karonda menggunakan model hewan uji embrio ikan zebra (Danio rerio). Pengujian toksisitas akut mengacu pada protokol OECD No. 236 tahun 2012 dan dilakukan pengamatan perubahan morfologi embrio ikan zebra yaitu tulang ekor, yolk sac dan pericardium. Hasil toksisitas fraksi n-heksana, etil asetat, dan air masing-masing menunjukkan nilai LC50 adalah 608,26 μg/mL; 571,78 μg/mL; dan 546,69 μg/mL. Hasil pengamatan perubahan morfologi pada usia 96 hours post fertilization (hpf) pada ketiga fraksi menunjukkan kelainan perubahan pada tulang ekor, edema yolk sac dan pericardium. Kategori ketoksikan bahan uji termasuk kategori tidak toksik.

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AKTIVITAS SITOTOKSIK EKSTRAK SELADA MERAH (Lactuca sativa var. Crispa) PADA BERBAGAI PELARUT EKSTRAKSI

Cited by 16 publications

References 7 publications

Antibacterial activity test on ethanol extract fraction of Kirinyuh (Chromolaena odorata L.) leaves for multi-drug resistant organisms bacteria

Antibacterial activity test on ethanol extract fraction of Kirinyuh (Chromolaena odorata L.) leaves for multi-drug resistant organisms bacteria

The Effect Of Mangrove Leaf Extract Dosage Sonneratia Alba On Hela Cell Viability

SKRINING TOKSISITAS AKUT BEBERAPA FRAKSI BUAH KARONDA (Carissa carandas L.) PADA EMBRIO ZEBRAFISH (Danio rerio)

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