Aktivitas Peroksidase Mutan Pisang Kepok dengan Ethyl Methane Sulphonate (EMS) secara In Vitro 1

Yanti, Yulmira

doi:10.31258/jnat.14.1.32-36

Cited by 7 publications

(8 citation statements)

References 5 publications

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“…Another reason for using this enzyme is due to the fact that this approach was very easy and simple to do as well as it is quite cheap in running this experiment. Besides, this oxidase enzyme had been recorded to have very high intensity on the plant organ especially during wounding time (Agrios 2005;Yanti 2011). This occurrence may have been correlation with defense mechanism of the plant, particularly when they grow under unfavorable environmental condition.…”

Section: Introductionmentioning

confidence: 98%

Evidence of pollen features and peroxidase isozymes in their morphological complexity of ten local cultivars of sweet potato from Indonesia

2019

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Abstract. Suranto, Wahyuni D, Edy Purwanto E. 2019. Evidence of pollen features and peroxidase isozymes in their morphological complexity of ten local cultivars of sweet potato from Indonesia. Biodiversitas 20: 2511-2518. Sweet potato (Ipomoea batatas Lam) has been recorded to have many cultivars and variations in the morphological characters not only for the leaves but also for the tubers. Sweet potatoes had economically significant role, especially as an additional food due to its high content of carbohydrate and other nutrient values. In order to test whether the morphological variation in this species was also expressed at the micro character; such as pollen morphology and enzyme activity, ten sweet potato cultivars were used in this study. The morphological characters such as shape and structure of the pollen features were examined using Scanning Electron Microscopy (SEM). Accordingly, Polyacrylamide Gel Electrophoresis (PAGE) was used to look-at the banding pattern of peroxidase isozymes. Morphological characters of leaves, flowers, and tuber were observed qualitatively both for the typical shape and colors. More detail of flower shapes was drown manually especially their petal edges. The results showed that there was variation among the ten sweet potato cultivars confirmed not only on their morphological characters but also the isozyme banding patterns of ten cultivars tested. At least, two cultivar Antin 3 and Papua Solossa had uniques characters especially the purple stem and yellow tuber and dark skin on the tuber, respectively. In addition, the structural analysis of pollen showed that Beta 1 cultivar has very large pollen; 116,50 μm (axis polar) and the contour surface of exine pollen was concave with the aperture shape and ornamentation were prolate spheroidal and echinate respectively. This unique character was also shown by its peroxidase isozyme bands. Eleven bands of leaf and 13 bands of stem samples were detected respectively. The typical Rf values of 0.31, 0.48 and 0.41 were detected for the leaf, while for stem samples were 0.14, 0.20 and 0.26. The Beta 1 cultivar showed very thick bands in between Rf values of 0.13 and 0.26. Meanwhile, the unique band pattern of Papua Salosa was the absence of band at 0.38.

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Section: Introductionmentioning

confidence: 98%

Evidence of pollen features and peroxidase isozymes in their morphological complexity of ten local cultivars of sweet potato from Indonesia

2019

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“…Pelepasan timbal ke dalam sitoplasma menghambat kerja enzim asam delta aminolevulinat hidratase dan profobilinogenase yang terlibat dalam biosintesis klorofil (Connel & Miller, 2006). Enzim peroksidase mempunyai fungsi yang berkaitan dengan proses ketahanan (Yanti, 2011). Enzim antioksidatif tanaman terhadap cekaman lingkungan yakni Superoxide dismutase (SOD), peroksidase (POX) dan Katalase (CAT) (Erdal & Demirtas, 2010 (Antari & Sundra, 2002), hal tersebut mempengaruhi penyerapan timbal oleh daun (Sulasmini et.al., 2007).…”

Section: Pendahuluanunclassified

Aktivitas Enzim Peroksidase dan Kadar Klorofil pada Daun Angsana (Pterocarpus indicus) sebagai Peneduh Jalan yang terpapar Timbal

Ferdhiani¹,

Lestari²,

Proklamasiningsih³

2019

Preprint

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One of emissions in the air that's harmful to human health is lead (Pb). The negative effects caused by lead and then required solutions for reducing the metal, its called phytoremediation. The Plant which acts as an agent of phytoremediation is Angsana (Pterocarpus indicus Willd.). Research to find out the lead content in Angsana's leaf at four locations, knowing the influence of exposure to lead against the activity of an enzyme peroxydases and chlorophyll content in the Angsana's leaf. The location for collection of samples conducted in four location at Purwokerto (1) H.R Bunyamin, (2) Ovis Isdiman, (3) Gerilya, and (4) Dr. Soeharso. A method of the sample used purposive sampling. Sample were analysed using wet digestion method, then were analysed using Atomic Absorbance Spectrofotometer (AAS). The activity of peroxydases and levels of chlorophyll were analysed using spectrophotometer Uv Vis. Result showed that the lead content in Angsana's leaf is 0,1 – 0, 25 mg/L, the activity of peroxidases is 600 - 1250 unit/g and chlorophyll content is 9,0 - 21,0 mg/L. The conclusion was no relations about chlorophyll content with levels of lead content and the relations about activity of enzymes peroxydases and lead content is low . Content of lead on Angsana's leaf of research has been done do not reach 1000 mg/L.

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“…yang diimbas asam salisilat secara in vitro menunjukkkan peningkatan yang signifikan dibandingkan dengan kontrol (0 ppm). Yanti (2011) melaporkan bahwa terjadi peningkatan variasi aktivitas enzim peroksidase pada bibit pisang kepok yang diberi perlakuan mutagen Ethyl Methane Sulphonate (EMS) secara in vitro. Menurut Agrios (2005), tanaman yang peka tidak mengalami perubahan atau bahkan mengalami penurunan aktivitas enzim peroksidase dibandingkan dengan tanaman dalam keadaan sehat, sedangkan tanaman yang tahan akan mengalami peningkatan.…”

Section: Muslimahunclassified

Aktivitas Enzim Peroksidase Daun Planlet Pisang Ketan (Musa paradisiaca L.) Hasil Pengimbasan Ketahanan terhadap Asam Salisilat secara In Vitro

Muslimah

Nurcahyani

Zulkifli

2017

JPPT

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Banana plant production are decreased because it getting an attack from Fusarium oxysporum. "ketan" Banana plantlet that resistant to the Fusarium oxysporum were selected by in vitro in the solid Murashige and Skoog (MS) medium with added salicylic acid at 40 ppm, 50 ppm, 60 ppm, and 70 ppm concentration, compared with controls (0 ppm). The aim of this research is to determine the activity of peroxidase enzyme on leaves of banana ketan plantlet. The research was carried out in December 2015 to February 2016 in the Laboratory of Botani (in vitro room), Departement of Biology, Faculty of MIPA, Lampung University. This study used a completely randomized design with 5 replications. Data were analyzed with the variance (Anova) and if them different will be continued by LSD test performed at 5% significance level. Extraction of peroxidase method using Saravanan et al. (2004) with the mixture of pyrogallol and 2 2. The absorbance was measured with a spectrophotometer (Shimudzu UV 800) at wavelength (λ) of 420 nm and readable from zero. The result showed that the activity of peroxidase enzyme on the leaves banana ketan plantlet has increased optimally in the salicylic acid concentration of 50 ppm compared with controls. Increased of Peroxidase enzyme activity showed that banana ketan plantlet was resistant to the salicylic acid and expected to be resistant to Fusarium oxysporum. PENDAHULUANPisang merupakan bahan pangan ke empat terpenting di dunia setelah beras, susu, dan gandum (Megia, 2005). Selain itu, pisang menjadi komoditas buah tropis yang sangat populer di dunia karena rasanya lezat, kandungan gizi tinggi, dan harga relatif murah (Sunarjono, 2004). Salah satu satu jenis pisang yang unggul di Indonesia yaitu pisang ketan (Mulyani et al., 2008).Kendala utama yang sering dihadapi oleh petani pisang Indonesia yaitu layu fusarium yang disebabkan oleh Fusarium oxysporum f.sp cubense (Foc) (Dimyati et al., 2000). Fusarium oxysporum merupakan jamur yang bersifat tular tanah (soil-borne pathogen). Jamur ini dapat menular melalui tanah atau dari tanaman sakit lain dan menginfeksi tanaman melalui luka pada akar sehingga menyebabkan penyakit layu (Semangun, 1989).

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Aktivitas Peroksidase Mutan Pisang Kepok dengan Ethyl Methane Sulphonate (EMS) secara In Vitro 1

Cited by 7 publications

References 5 publications

Evidence of pollen features and peroxidase isozymes in their morphological complexity of ten local cultivars of sweet potato from Indonesia

Evidence of pollen features and peroxidase isozymes in their morphological complexity of ten local cultivars of sweet potato from Indonesia

Aktivitas Enzim Peroksidase dan Kadar Klorofil pada Daun Angsana (Pterocarpus indicus) sebagai Peneduh Jalan yang terpapar Timbal

Aktivitas Enzim Peroksidase Daun Planlet Pisang Ketan (Musa paradisiaca L.) Hasil Pengimbasan Ketahanan terhadap Asam Salisilat secara In Vitro

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