Aktivitas Enzim Selulase Mikroba Yang Diisolasi Dari Jerami Padi Di Persawahan Pasang Surut Di Kalimantan Selatan

Razie, Fakhrur; Anas, Iswandi; Sutandi, Atang; Gunarto, Lukman; Sugiyanta, Sugiyanta

doi:10.29244/jitl.13.2.43-48

Cited by 8 publications

(5 citation statements)

References 7 publications

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“…Based on the Tukey Honest Significant Difference (HSD) test at the α level of 5%, a similar letter denotes that there is no difference between the treatment's effects The change of condition from reductive to oxidative causes a change in the load of the redox elements. The content of the elements ferro (Fe 2+ ), and manganese (Mn 2+ ) transformed into more stable ferri (Fe 3+ ) and mango (Mn 4+ ), binding hydroxide ions (OH -) and releasing hydrogen ions, as well as the transformation of sulfide (S 2-) into sulfate (SO 4 2-), which gave the effect of soil saturation [25]. The condition of the reductive (Eh) soil will decrease, while the condition of the dry (oxidative) Eh soil will increase.…”

Section: Yield and Productionmentioning

confidence: 99%

Changes in Soil Ph and Eh Of Rainfed Rice Fields With The Application of NPK Fertilizer and Rice Straw Compost

Saputra,

Jumar,

Norwinda

2023

BIO Web Conf.

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In Indonesia, rainfed rice fields (non-irrigated) account for only 26.5% of total rice production. The use of sub-optimal land, such as rainfed rice fields, can be used as an alternative. In general, these rainfed rice fields were infertile, they often experienced drought, and their irrigation systems depended on rain. pH and Eh soil are the main factors affecting the solubility and availability of nutrients and their transformation in the soil. Thusly, the purposes of this research were to determine the interaction effect of NPK fertilizer combined with rice straw compost on changes in soil pH and Eh in rainfed rice fields planted with rice. This research used a two-factor randomized block design (RBD). NPK fertilizer dosage (a) and composted rice straw dosage (k) made up the first and second factors, respectively. There were 3 treatments of NPK fertilizer doses, i.e. 0 (control); 150; and 300 kg ha-1, while there were 6 doses of rice straw compost, 5; 7.5; 10; 12.5; 15; and 20 t ha-1. Each treatment consisted of two experimental blocks, resulting in 36 experimental units. Soil observations were carried out three times, namely when the rice was 2 weeks after planting (WAP), 10 WAP, 17 WAP. Results showed the best treatment combination for lowering Eh soil at 2 WAP was a2k4 (300 kg ha-1 + 12.5 t ha-1 rice straw compost) with a value of Eh -251,2 mV. The effect of single-factor compost was best in increasing the soil pH at 2 WAP, which was 15 t ha-1 (k5) with a pH value of 7.28.

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Section: Yield and Productionmentioning

confidence: 99%

Changes in Soil Ph and Eh Of Rainfed Rice Fields With The Application of NPK Fertilizer and Rice Straw Compost

Saputra,

Jumar,

Norwinda

2023

BIO Web Conf.

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“…Hal ini dapat terjadi karena Aspergillus niger dapat menghasilkan enzim selulase yang merombak selulosa menjadi selubiosa hingga akhirnya menjadi glukosa sehingga meningkatkan energi dan mudah untuk dicerna. Razie, et al (2011) Berdasarkan Tabel 4 diketahui bahwa hasil uji Duncan menunjukkan perlakuan P0 berbeda nyata dengan perlakuan P4, tetapi tidak berbeda nyata dengan perlakuan P1, P2, dan P2 tidak berbeda nyata dengan P3. Perlakuan P1 berbeda nyata dengan perlakuan P4 tetapi tidak berbeda nyata dengan perlakuan P2.…”

Section: Jurnal Riset Dan Inovasiunclassified

PENGARUH CAMPURAN DAUN SINGKONG ONGGOK FERMENTASI MENGGUNAKAN Aspergillus niger TERHADAP BAHAN KERING, ABU, BAHAN ORGANIK, SERAT KASAR, DAN PROTEIN KASAR

Nusantara

Sutrisna²,

Muhtarudin³

et al. 2022

JRIP

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Penelitian ini bertujuan untuk mengetahui pengaruh campuran daun singkong onggok fermentasi menggunakan Aspergillus niger terhadap kualitas bahan kering, abu, bahan organik, serat kasar, dan protein kasar. Penelitian ini dilaksanakan pada Desember 2021--Februari 2022 di Laboratorium Nutrisi dan Makanan Ternak, Jurusan Peternakan, Fakultas Pertanian, Universitas Lampung. Rancangan percobaan yang digunakan adalah Rancangan Acak Lengkap (RAL) dengan 5 perlakuan dan 3 ulangan. Perlakuan yang diberikan yaitu onggok 50% + daun singkong 50% tanpa fermentasi (P0), onggok 20% + daun singkong 80% + Aspergillus niger 1% BK (P1), onggok 30% + daun singkong 70% + Aspergillus niger 1% BK (P2), onggok 40% + daun singkong 60% + Aspergillus niger 1% BK (P3), dan onggok 50% + daun singkong 50% + Aspergillus niger 1% BK (P4). Data yang diperoleh dianalisis menggunakan analisis ragam dengan taraf nyata 1% atau 5% dan dilanjutkan uji berganda Duncan. Hasil penelitian didapatkan fermentasi dengan Aspergillus niger 1% BK dapat menurunkan kandungan serat kasar serta dapat menigkatkan kandungan protein kasar campuran onggok dan daun singkong fermentasi. Respon terbaik pada campuran onggok dan daun singkong terhadap kualitas bahan organik, serat kasar, dan protein kasar didapatkan pada campuran onggok 40% + daun singkong 60% + Aspergillus niger 1% BK yang menghasilkan bahan organik sebesar 40,20% dengan rendah serat kasar sebesar 13,39% dan menghasilkan protein kasar sebesar 24,32%.

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“…The blank contained 1 mL of 100 mM phosphate buffer pH 6 and 1.5 mL of DNS solution. The CMCase activity test was carried out two times [11,19]. The value of enzyme activity is determined based on the following calculations [11]:…”

Section: Cmcase Activity Assaymentioning

confidence: 99%

Tobacco Stalk as Source of CMCase Enzyme Production of Actinomycetes Isolated from Rhizosphere of Tobacco (Nicotiana tabacum L.) by Submerged Fermentation

Utarti¹,

Jannah²,

Arimurti³

2022

Proceedings of the 4th International Conference on Life Sciences and Biotechnology (ICOLIB 2021)

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Tobacco stalk is tobacco plant waste which contains about 56% cellulose so that it is possible to use it as a substrate for the production of cellulase, namely CMCase. The study conducted in several stages, namely screening the cellulolytic activity of 71 actinomycetes isolates, optimizing CMCase production by three actinomycetes with the best CMCase activity index using tobacco stalk as substrate through submerged fermentation, and morphology identification actinomycetes with the highest CMCase activity. A total of 71 actinomycetes have been isolated from the rhizosphere of tobacco plant. In this study, the ability of actinomycetes to produce cellulase through submerged fermentation using tobacco stalk substrate powder. There were about 71 actinomycetes isolated and all of them have cellulolytic activities. Based on the screening of cellulolytic activity on CMC (carboxymethyl cellulose) agar plate, the enzyme activity index of ATG 38 isolate was higher than others, with 2.50 ± 0.26 respectively. The cellulase production using tobacco stalk as substrate through submerged fermentation was optimal showing that CMCase production of actinomycete ATG 38 isolates maximal after four days incubation with 0,40 mU/mL enzyme activity. Microscopic morphological observations showed that actinomycete ATG 38 isolate belongs to the genus Streptomyces.

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Aktivitas Enzim Selulase Mikroba Yang Diisolasi Dari Jerami Padi Di Persawahan Pasang Surut Di Kalimantan Selatan

Cited by 8 publications

References 7 publications

Changes in Soil Ph and Eh Of Rainfed Rice Fields With The Application of NPK Fertilizer and Rice Straw Compost

Changes in Soil Ph and Eh Of Rainfed Rice Fields With The Application of NPK Fertilizer and Rice Straw Compost

PENGARUH CAMPURAN DAUN SINGKONG ONGGOK FERMENTASI MENGGUNAKAN Aspergillus niger TERHADAP BAHAN KERING, ABU, BAHAN ORGANIK, SERAT KASAR, DAN PROTEIN KASAR

Tobacco Stalk as Source of CMCase Enzyme Production of Actinomycetes Isolated from Rhizosphere of Tobacco (Nicotiana tabacum L.) by Submerged Fermentation

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