Tobacco stalk is tobacco plant waste which contains about 56% cellulose so that it is possible to use it as a substrate for the production of cellulase, namely CMCase. The study conducted in several stages, namely screening the cellulolytic activity of 71 actinomycetes isolates, optimizing CMCase production by three actinomycetes with the best CMCase activity index using tobacco stalk as substrate through submerged fermentation, and morphology identification actinomycetes with the highest CMCase activity. A total of 71 actinomycetes have been isolated from the rhizosphere of tobacco plant. In this study, the ability of actinomycetes to produce cellulase through submerged fermentation using tobacco stalk substrate powder. There were about 71 actinomycetes isolated and all of them have cellulolytic activities. Based on the screening of cellulolytic activity on CMC (carboxymethyl cellulose) agar plate, the enzyme activity index of ATG 38 isolate was higher than others, with 2.50 ± 0.26 respectively. The cellulase production using tobacco stalk as substrate through submerged fermentation was optimal showing that CMCase production of actinomycete ATG 38 isolates maximal after four days incubation with 0,40 mU/mL enzyme activity. Microscopic morphological observations showed that actinomycete ATG 38 isolate belongs to the genus Streptomyces.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.