Aktivitas Antioksidan serta Penghambatan HMG CoA dan Lipase dari Kombinasi Ekstrak Daun Binahong-Rimpang Temu Lawak

Abstract

The prevalence of dyslipidemia in Indonesia continues to increase. Free radicals are increasing the occurrence of diseases, one of them is dyslipidemia. Previous studies showed that antioxidant compounds have strong potential as dyslipidemia drug. The mechanism action of dyslipidemia treatment is inhibiting cholesterol synthesis through the HMG CoA reductase and cholesterol absorption mediated with lipase. This study aimed to analyze the antioxidant activity along with the inhibition of HMG CoA reductase and lipase in a combination of ethanolic extract of Anredera cordifolia leaf (EB) with ethanolic extract of Curcuma xanthorrhiza rhizome (ET) and its single form. The antioxidant activity test was conducted using DPPH method by making serial dilution of samples and ascorbic acid with adding DPPH and methanol as standard. The inhibitory activity of HMG CoA and lipase was conducted by making a series concentration of sample as well as simvastatin as the comparison. The results showed that the highest antioxidant activity is in ET samples IC50 16.21 ± 1.74 µg/mL, but was less potential compared to ascorbic acid IC50 7,63±0,85 µg/mL. The most potential inhibition of HMG CoA and lipase is in ET samples IC50 8.35 ± 0.02 and 22.35 ± 1.26 µg/mL. The combination of ET-EB (1: 1) is better than EB but lower than ET in antioxidant activity as well as the inhibition of HMG CoA reductase and lipase. The result showed a correlation that the higher antioxidant activity, the higher inhibitory of HMG CoA reductase and lipase respectively.

The Antioxidant Effects of the Ethanolic Extract of Binahong Leaves Unilateral Ureteral Obstruction Rat Model

“…The extract from the leaves of binahong (Anredera cordifolia (Ten) Steenis) is known to contain flavonoids that have antioxidant activity ward off free radicals. 6 A dose of 150 mg/kg BW binahong leaf ethanolic extract improved renal function in gentamicin-piroxicam-induced renal disease model rats in previous research. The current study aims to determine the impact of binahong leaves against chronic kidney damage in Sprague Dawley rats using unilateral ureteral obstruction rats model.…”

In Silico and In Vitro Analysis of Tacca Tubers (Tacca leontopetaloides) from Banyak Island, Aceh Singkil Regency, Indonesia, as Antihypercholesterolemia Agents

“…The IC 50 value indicates the potential for inhibition of the HMG-CoA reductase enzyme, where the smaller the IC 50 value, the greater the inhibition potential for the activity of the HMG-CoA reductase enzyme [ 48 ]. In vitro testing using the HMG-CoA reductase assay kit showed that the ethanol extract from Tacca tubers acted as an inhibitor of HMG-CoA reductase with an IC 50 value of 4.92 ppm, which was close to the IC 50 value of simvastatin by comparison (4.62 ppm).…”

Effect of Bajakah Tea Extract (Spatholobus littoralis Hassk) on High Density Lipoprotein, Triglyceride and Total Cholesterol Levels in Male Wistar Rats

“…This drug is an option for treating hyperlipidemia, which has a mechanism of action as an inhibitor of the HMG-CoA reductase enzyme. 14 The mechanism of action in the body by inhibiting 3-hydroxy-3-methylglutaryl HMG-CoA reductase is also responsible for converting HMG-CoA into mevalonate. In the process, it will reduce LDL by 50% and triglyceride levels in the blood.…”