AKTIVITAS ANTIBAKTERI FERMENTAT FUNGI ENDOFIT DAUN KASUMBA TURATE (Carthamus tinctorius L.) ASAL GALESONG TERHADAP BAKTERI UJI PENYEBAB INFEKSI KULIT

Abstract

Kasumba turate (Carthamus tinctorius L.) contains phenolic compounds of flavonoids and carotenoids which have antioxidant, antibacterial, antiviral, anti-inflammatory, antiallergic and anticancer activities. This study aims to obtain isolates of endophytic fungi that have potential as antibacterial using the TLC-Bioautography method. This research was conducted by isolating endophytic fungi from the leaves of kasumba turate (Carthamus tinctorius L.) and after that purification and macroscopic tests were carried out and eight pure isolates were obtained. The results of the isolate screening tests with IFDKT 5 and IFDKT 6 codes showed the highest inhibition. Then tested the antibacterial activity by TLC-Bioautography. The isolates with IFDKT code 5 showed five spots were active against Staphylococcus aureus and Pseudomonas aeruginosa (Rf1 0.83; Rf2 0.65; Rf3 0.47; Rf4 0.30; Rf5 0.07), and six spots were active against bacteria Staphylococcus epidermidis (Rf1 0.94; Rf2 0.83; Rf3 0.65; Rf4 0.47; Rf5 0.30, Rf6 0.07). Then, the isolates with code IFDKT 6 showed four active spots against Staphylococcus aureus and Pseudomonas aeruginosa (Rf1 0.80; Rf2 0.58; Rf3 0.40; Rf4 0.10), and five spots were active against Staphylococcus epidermidis (Rf1 0.96; Rf2 0.80; Rf3 0.58; Rf4 0 .40; Rf5 0.10). Therefore, fermentate isolate of the endophytic fungus of kasumba turate leaves (Carthamus tinctorius L.) has the potential to inhibit Staphylococcus aureus, Stahpyloccous epidermidis and Pseudomonas aeruginosa bacteria that cause skin infections.