Aktivitas Antibakteri Ekstrak Akar Ceplukan (Physalis angulata L.) Terhadap Staphylococcus epidermidis dan Pseudomonas aeruginosa

Abstract

Root ceplukan (Physalis angulata L.) is one of the medicinal plants that contain several active compounds that are antibacterial. This study aims to prove the antibacterial activity of root ceplukan extract againsts Staphylococcus epidermidis bacteria and Pseudomonas aeruginosa bacteria. Root ceplukan was extracted by maceration with ethanol 70%. Root root extracts of phytochemical screening include flavonoids, alkaloids and saponins. The ceplukan root extract was then tested against S. epidermidis and P. aeruginosa bacteria using wells method with concentrations of 20%, 40%, 60%, 80%, 100%, aquades as a negative control and amikacin as a positive control. Inhibitory zone diameter with each concentration for P. aeruginosa bacteria, among others, negative control = 0 mm, positive control = 21,623 mm, 100% = 16.9 mm, 80% = 15.05 mm, 60% = 13.703 mm, 40% = 12.02 mm, 20% = 12.02 mm and S. epidermidis bacteria have inhibitory zone at positive control of 21,623 mm. The results of this study showed that root extract ceplukan proved to have inhibition zone against bacteria P. aeruginosa at all concentrations but has no inhibition zone against S. epidermidis bacteria. Root ceplukan extract has a minimum inhibitory concentration of 14% and a minimum bacterisid concentration of 20%, against P. aeruginosa bacteria. The results of phytochemical analysis showed that root ceplukan positively contain flavonoids, alkaloids and saponins are suspected as antibacterial compounds.
 
 Keywords : Antibacterial, Staphylococcus epidermidis, Pseudomonas aeruginosa, Ceplukan root extract

Antibacterial Activity and Bioautography of the Chloroform Fraction of Morel Berry (Physalis angulata L.) Root Against Staphylococcus epidermidis and Pseudomonas aeruginosa

“…The plate was removed from the chamber and observed with a 254 nm and a 366 nm UV lamp. Visible stains were detected with Bouchardat spray reagent for alkaloids (brown color), ammonia for flavonoids (yellow brown/ green/brown/pink color), FeCl 3 for tannins (strong green, red, purple, blue or black color) and Liebermann-Burchard for saponins (purple color) [6]. Furthermore, the measurement of the retention factor (Rf) spots in the chromatogram from the TLC results was carried out.…”

“…Morel berry roots contain active compounds including alkaloids, flavonoids, and saponins [6]. Spots on the chloroform fraction of morel berry roots by looking at the results of the ammonia spray test can be stated to contain flavonoid compounds with Rf 0.36, 0.44, 0.60, and 0.84.…”

“…Various research results state that morel berry root has shown antibacterial activity. The ethanol extract of the morel berry root was screened for phytochemicals positive for flavonoids, alkaloids and saponins and showed bactericidal ability against Pseudomonas aeruginosa bacteria at a concentration of 20% with an inhibition zone diameter of 10.01 mm [6]. The n-hexane, ethyl acetate and water fractions of morel berry herbal have antibacterial activity against Staphylococcus epidermidis [7].…”

Bioautography and FTIR Analysis of Ethanol Fraction Morel Berry Root (Physalis angulata L.) Against Staphylococcus epidermidis and Pseudomonas aeruginosa

“…With inhibitory power at 14% MIC and MBC at 20%. Whereas for S. epidermidis morel berry root extract has no antibacterial activity (Viogenta et al, 2017). Based on the phytochemical test results from morel berry root extract, it is known to contain flavonoid compounds, tannins, saponins, and alkaloids which function as antibacterial (Viogenta et al, 2017).…”

“…Whereas for S. epidermidis morel berry root extract has no antibacterial activity (Viogenta et al, 2017). Based on the phytochemical test results from morel berry root extract, it is known to contain flavonoid compounds, tannins, saponins, and alkaloids which function as antibacterial (Viogenta et al, 2017). In morel berry root extract, the compound content is still mixed between polar, semipolar, and nonpolar properties so that compounds that do not have the potential to be antibacterial are also extracted (Viogenta et al, 2017).…”