Aktivitas Anti-Inflamasi In Vitro Ekstrak Etanol Daun Vernonia amygdalina DELILE Dengan Pengujian Stabilisasi Membran

Nuryanto, Muhammad Khairul; Paramita, Swandari; Iskandar, Abdillah; Ismail, Sjarif; Ruslim, Andre Kusuma

doi:10.25026/jsk.v1i8.80

Cited by 2 publications

(1 citation statement)

References 3 publications

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“…Meskipun inflamasi merupakan proses yang terjadi secara alamiah dalam tubuh, namun jika dibiarkan dan terjadi secara berlebihan dapat menimbulkan efek yang kurang baik terhadap tubuh sehingga perlu dilakukan pengobatan dengan cara mengurangi terjadinya inflamasi (5). Salah satu upaya yang dapat dilakukan untuk mengurangi terjadinya inflamasi dalam tubuh adalah dengan menghambat COX-2, sehingga pembentukan prostaglandin sebagai mediator inflamasi berkurang atau bahkan tidak terjadi (6).…”

Section: A Pendahuluanunclassified

Uji Aktivitas Antiinflamasi Senyawa Turunan Asetogenin pada Daun Sirsak (Annona muricata L.) terhadap Reseptor Siklooksigenase-2 (COX-2) secara In Silico

Siti Ainun Rohaniah,

Mulyanti,

Fakih

2023

BCSP

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Abstract. Inflammation occurs naturally in the body as a response to foreign molecules. The inflammatory process occurs due to the release of inflammatory mediators catalyzed by enzymes, one of which is the COX-2 enzyme. Although it is a response from the body, if left unchecked, inflammation can cause unwanted effects. The use of steroids and non-steroidal anti-inflammatory drugs (NSAIDs) which often cause side effects is the reason for using natural ingredients as an alternative treatment. Soursop leaves (Anonna muricata L.) have been used empirically as medicinal plants because of their properties, one of which is as an anti-inflammatory with a mechanism of action that inhibits the COX-2 enzyme. Acetogenin is an active compound contained in soursop leaves with various compound derivatives. The purpose of this study is to determine the activity of 9 acetogenin derivative compounds to inhibit COX-2 enzyme using molecular docking method in silico. The in silico assay is a computational assay that interacts the test compound with the target receptor with the results in the form of bond free energy values (∆G) and inhibition constants (Ki). The results showed that the compound annomuricin C has the closest binding free energy value to the natural ligand of -5.71 kcal/mol with an inhibition constant of 64.92 uM. Abstrak. Inflamasi terjadi secara alami dalam tubuh sebagai respon terhadap molekul asing. Proses inflamasi terjadi karena adanya pelepasan mediator inflamasi yang dikatalisasi oleh enzim salah satunya adalah enzim COX-2. Meskipun merupakan respon dari tubuh, jika dibiarkan inflamasi dapat menimbulkan efek yang tidak diinginkan. Penggunaan obat antiinflasmasi steroid dan non-steroid (AINS) yang seringkali menimbulkan efek samping menjadi alasan penggunaan bahan alam sebagai alternatif pengobatan. Daun sirsak (Anonna muricata L.) telah digunakan secara empiris sebagai tanaman obat karena khasiatnya yang dihasilkan, salah satu diantaranya adalah sebagai antiinflamasi dengan mekanisme kerja menghambat enzim COX-2. Asetogenin merupakan senyawa aktif yang terkandung pada daun sirsak dengan berbagai turunan senyawa. Tujuan dari penelitian ini adalah untuk mengetahui aktivitas terhadap 9 senyawa turunan asetogenin untuk menghambat enzim COX-2 dengan menggunakan metode molecular docking secara in silico. Uji in silico merupakan pengujian secara komputasi yaitu menginteraksikan senyawa uji dengan reseptor target dengan hasil berupa nilai energi bebas ikatan (∆G) dan konstanta inhibisi (Ki). Hasil penelitian menunjukkan bahwa senyawa annomuricin C memiiki nilai energi bebas ikatan yang paling besar yaitu sebesar -5,71 kcal/mol dengan konstanta inhibisi sebesar 64,92 uM.

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Section: A Pendahuluanunclassified

Uji Aktivitas Antiinflamasi Senyawa Turunan Asetogenin pada Daun Sirsak (Annona muricata L.) terhadap Reseptor Siklooksigenase-2 (COX-2) secara In Silico

Siti Ainun Rohaniah,

Mulyanti,

Fakih

2023

BCSP

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Suppression of Proinflammatory Cytokines by Etlingera alba (A.D.) Poulsen Rhizome Extract and Its Antibacterial Properties

Hamsidi

Wahyuni

Sahidin

et al. 2021

Advances in Pharmacological and Pharmaceutical Sciences

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Etlingera alba is one of the Etlingera plants that has not been studied intensively. Plants that belong to the same genus have similar constituents and pharmacological activities. Thus, we aim to investigate the chemical composition and pharmacological activities, namely, anti-inflammatory and antibacterial properties, of E. alba rhizome extract (EA). The chemical constituent was detected using the test tube method. The inflammatory model rats were obtained by inducing them with 1% carrageenan, and their palm edema volume and cytokine levels, namely, IL-6, IL-12, and TNF-α, were measured. Antibacterial activity was performed with broth microdilution. The phytochemical screening of EA was detecting alkaloids, flavonoids, tannins, steroids, and phenols. The EA has anti-inflammatory activity by reducing the palms’ edema volume and cytokine levels (IL-6, IL-12, and TNF-α), and the optimal concentration was 400 mg/kg body weight (BW). On the other hand, EA also exhibited antibacterial properties against E. coli and S. enterica. In conclusion, similar to other Etlingera plants, EA also demonstrates pharmacological activities, namely, anti-inflammatory and antibacterial properties.

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Aktivitas Anti-Inflamasi In Vitro Ekstrak Etanol Daun Vernonia amygdalina DELILE Dengan Pengujian Stabilisasi Membran

Cited by 2 publications

References 3 publications

Uji Aktivitas Antiinflamasi Senyawa Turunan Asetogenin pada Daun Sirsak (Annona muricata L.) terhadap Reseptor Siklooksigenase-2 (COX-2) secara In Silico

Uji Aktivitas Antiinflamasi Senyawa Turunan Asetogenin pada Daun Sirsak (Annona muricata L.) terhadap Reseptor Siklooksigenase-2 (COX-2) secara In Silico

Suppression of Proinflammatory Cytokines by Etlingera alba (A.D.) Poulsen Rhizome Extract and Its Antibacterial Properties

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