Agrotransformación y evaluación de la resistencia a Phytophthora infestansen Solanum tuberosum L. variedad Désirée

Orbegozo, Jeanette; Roman, M.; Rivera, Cristina; Tovar, Jose C.; Pérez, W.; Gamboa, Soledad; Forbes, G. A.; Kreuze, Jan; Ghislain, Marc

doi:10.15381/rpb.v20i3.5202

Cited by 2 publications

(2 citation statements)

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“…The Rpi-blb2 gene isolated from S. bulbocastanum (wild potatoes in Mexico) was transformed to cv. "Desiree" via agro-infection and it conferred high resistance against the late blight disease in the transgenics with high dose inoculation of two isolates of P. infestans to the entire transgenic plant, demonstrated by the unsuccessful reinoculation event of P. infestans in the transgenic potatoes (Orbegozo et al, 2016). With the use of the oxalate oxidase 4 gene (Osoxo4) isolated from rice overexpressed and transformed in S. tuberosum L. cvr Chipsona 3 via Agrobacterium mediated transformation under the regulation of 35S promoter, elevated activity of the enzyme oxalate oxidase was demonstrated in the transgenic potatoes with higher level of reactive oxygen H 2 O 2 .…”

Section: Phytophthora and Verticillium Resistant Potatoesmentioning

confidence: 99%

“…The transgenic potato lines exhibited immunity against both PVX and PVY (Bai et al, 2009). Orbegozo et al (2016) reported development of transgenic potatoes cv. "Desiree" resistant against Potato leafroll virus (PLRV) using self-excisable Cre-LoxP in vector that contained inverted repeats of CP gene under the control of heat inducible promoter and nptII as selection marker.…”

Section: Potatoes With Virus Resistancementioning

confidence: 99%

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GENETIC APPROACHES FOR ENGINEERING BIOTIC STRESS RESISTANCE IN POTATO (Solanum tuberosum L.)

Bakhsh¹,

Dangol²,

Naeem³

et al. 2020

THE JAPS

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Potato is one of the most important food crops in terms of annual production and food security worldwide. The crop is affected by several types of biotic stresses, e.g. insects, viruses, fungus, nematodes and weeds, which are the prominent limiting factors for its production. The conventional breeding methods in potato have been associated with limitations; none of the present day commercial cultivar has built-in resistance against biotic stresses. There is strong need for the development of new resistant potato varieties to cope against biotic stresses using non-classical approaches in combination with classical methods. The scientific literature suggests the contribution of modern biotechnological techniques for the development of transgenic potato lines resistant against insects and diseases. The present comprehensive review describes different genetic engineering approaches for the development of transgenic potatoes resistant to insects, weeds, nematodes, fungus and viruses by fellow researchers worldwide. It also gives an insight into modern technologies, e.g. RNAi and CRISPR-Cas9, which have emerged recently and can be implemented in the development of biotic stress resistant potato cultivars.

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Section: Phytophthora and Verticillium Resistant Potatoesmentioning

confidence: 99%

Section: Potatoes With Virus Resistancementioning

confidence: 99%