Agrobacterium-mediated transient transformation of Mexican prickly poppy (Argemone mexicana L.)

Godoy-Hernandez, Gregorio; Aviles-Berzunza, Elide; Carrillo-Pech, Mildred; Vázquez‐Flota, Felipe

doi:10.2225/vol11-issue1-fulltext-3

Cited by 5 publications

(2 citation statements)

References 7 publications

(10 reference statements)

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“…Positive GUS activity could be solely attributed to the expression and correct splicing of the inserted uidA in eukaryotic plant cells. P. andrieuxii does not present endogenous GUS activity as reported for carrot, celery, parsley, rice [13], Ricinus communis [14], Agave fourcroydes [15] and Capsicum chinense [16]. Older roots displayed a higher GUS activity than younger root explants and leaf sections.…”

Section: Transient Transformation Of Pentalinon Andrieuxiisupporting

confidence: 56%

Agrobacterium-mediated transient transformation of Pentalinon andrieuxii Müll. Arg.

Yam-Puc¹,

Avilés-Berzunza²,

Chan-Bacab³

et al. 2012

ABB

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Sections of hypocotyls, roots and leaves from Pentalinon andrieuxii plantlets were transiently transformed with Agrobacterium tumefaciens LBA4404 bearing the binary plasmid pCAMBIA2301 with an interrupted β-glucuronidase (GUS) gene. Histochemical GUS assays showed transient gene expression in all infected tissues, being older roots those which displayed the most intense GUS staining. To our knowledge, this is the first report of Pentalinon andrieuxii susceptibility to Agrobacterium tumefaciens-mediated genetic transformation.

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Section: Transient Transformation Of Pentalinon Andrieuxiisupporting

confidence: 56%

Agrobacterium-mediated transient transformation of Pentalinon andrieuxii Müll. Arg.

Yam-Puc¹,

Avilés-Berzunza²,

Chan-Bacab³

et al. 2012

ABB

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“…(4) Incubate at 37 C for 1 h to overnight. (5) After incubation, rinse in 70 % ethanol for 5 min (Ohta et al 1990;Vitha et al 1995;Stangeland and Salehian 2002;Sun et al 2006;Godoy-hernández et al 2008). …”

Section: Histochemical Gus Assaymentioning

confidence: 99%

Nanotechnologies in Food and Agriculture

Rai¹,

Ribeiro²,

Mattoso³

et al. 2015

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Nanoparticles-Based Delivery Systems in Plant Genetic Transformation

Rai

Bansod

Bawaskar

et al. 2015

Nanotechnologies in Food and Agriculture

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The production of transgenic plants is considered as a valuable tool in plant research and the technology is comprehensively useful in agricultural research. Gene transfer in plants is generally carried out by Agrobacterium sp., application of some chemicals, and physical techniques (electroporation, microprojectile, etc.). Now-a-days with better efficacy and stability, new methods for the gene transfer in plants are coming up. The advent of nanotechnology, the nanoparticles-based delivery systems for genetic transformation of plants, is coming in a big way.In this chapter, we have discussed the novel nanotechnologies like nucleic acidconjugated nanoparticles with their current status and future prospects in the development of gene transfer methods in plants. We have also highlighted the shortcomings of conventional techniques of gene transfer in plants and discussed the role of established nanotechnology and chemical-based strategy for surface

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Agrobacterium-mediated transient transformation of Mexican prickly poppy (Argemone mexicana L.)

Cited by 5 publications

References 7 publications

<i>Agrobacterium</i>-mediated transient transformation of <i>Pentalinon andrieuxii</i> Müll. Arg.

<i>Agrobacterium</i>-mediated transient transformation of <i>Pentalinon andrieuxii</i> Müll. Arg.

Nanotechnologies in Food and Agriculture

Nanoparticles-Based Delivery Systems in Plant Genetic Transformation

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Agrobacterium-mediated transient transformation of Mexican prickly poppy (Argemone mexicana L.)

Cited by 5 publications

References 7 publications

&lt;i&gt;Agrobacterium&lt;/i&gt;-mediated transient transformation of &lt;i&gt;Pentalinon andrieuxii&lt;/i&gt; M&#252;ll. Arg.

&lt;i&gt;Agrobacterium&lt;/i&gt;-mediated transient transformation of &lt;i&gt;Pentalinon andrieuxii&lt;/i&gt; M&#252;ll. Arg.

Nanotechnologies in Food and Agriculture

Nanoparticles-Based Delivery Systems in Plant Genetic Transformation

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Product

Resources

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<i>Agrobacterium</i>-mediated transient transformation of <i>Pentalinon andrieuxii</i> Müll. Arg.

<i>Agrobacterium</i>-mediated transient transformation of <i>Pentalinon andrieuxii</i> Müll. Arg.