Nanoparticles-Based Delivery Systems in Plant Genetic Transformation

Abstract

The production of transgenic plants is considered as a valuable tool in plant research and the technology is comprehensively useful in agricultural research. Gene transfer in plants is generally carried out by Agrobacterium sp., application of some chemicals, and physical techniques (electroporation, microprojectile, etc.). Now-a-days with better efficacy and stability, new methods for the gene transfer in plants are coming up. The advent of nanotechnology, the nanoparticles-based delivery systems for genetic transformation of plants, is coming in a big way.In this chapter, we have discussed the novel nanotechnologies like nucleic acidconjugated nanoparticles with their current status and future prospects in the development of gene transfer methods in plants. We have also highlighted the shortcomings of conventional techniques of gene transfer in plants and discussed the role of established nanotechnology and chemical-based strategy for surface

Nanobiotechnology for Plants

“…These methods are better efficacy and have the stable integration as well as the rapid expression for the transgenic process using nanoparticles (Rai et al 2015;Khodakovskaya and Lahiani 2016;Shukla et al 2016;Pacheco and Buzea 2017). These nanoparticles could be conjugated with nucleic acid developing the methodology of gene transfer in plants and improving the efficacy, stability and accuracy of transgenic process making it less time-consuming (Burlaka et al 2015;Rai et al 2015;Elahian et al 2017). Therefore, it could be concluded that, the encapsulation of nanomaterials could be considered the best method in delivering different agro-chemicals or different agricultural active ingredients in more efficient manner.…”

“…On the other hand, there are many applications of nanobiotechnology in agricultural sector including nano-encapsulated fertilizers (Chhipa 2016;Chhipa and Joshi 2016;Dubey and Mailapalli 2016;Khan and Rizvi 2017;López-Valdez and Fernández-Luqueño 2018) and pesticides (Kah et al 2014;Chhipa 2016;Chhipa and Joshi 2016;Dubey and Mailapalli 2016;Khan and Rizvi 2017;Abd-Elsalam and Prasad 2018) as well as nanoparticles mediated genetic material delivery in plants (Hatami et al 2016;Siddiqi and Husen 2017a;Tripathi et al 2017a). Furthermore, some delivery of mesoporous nanoparticles mediated DNA and proteins in plants have been proved (Rai et al 2015;Chung et al 2016;Rajan et al 2017).…”

“…So, the nanobiotechnology has a serious mission in improving crop production as reported in many studies (e.g., Balaure et al 2017). These studies focus on the transfer of response of protein and other chemical complexes mediated by certain nanoparticles into the plant cells (Brandelli 2015;Rai et al 2015;Álvarez et al 2016;Sarmast and Salehi 2016). Therefore, further investigations in the nanobiotechnology are needed to offer promising prospects in improving the production and management agricultural crops as well as the production of transgenic plants (Bhau et al 2016;Misra et al 2016;Balaure et al 2017;Siddiqi and Husen 2017a).…”

A Perspective on Hypericum perforatum Genetic Transformation

“…Viruses infecting H. perforatum (Kegler et al, 1999 ) and H. japonicum (Du et al, 2013 ) have been identified and characterized. Furthermore, nanoparticle mediated DNA delivery into plant cells is gaining momentum (Rai et al, 2015 ), which would also offer potential benefits in the genetic transformation of H. perforatum in the future.…”

Novel nanoplex‐mediated plant transformation approach

“…There are many established gene-transfer methods including Agrobacterium-mediated transformation, viral vectors, microinjection, biolistic or particle bombardment, PEGpolyethylene glycol and electroporation [1][2][3][4][5]. These methods do have some or the other limitations and are less efficient [6][7][8][9]. Many reports are also available on the gene-transfer by bioactive beads (BAB)-mediated transformation [10][11][12].…”