Agrobacterium-Mediated Transformation of Rice: Constraints and Possible Solutions

Mohammed, Sulaıman; Samad, Azman Abd; Rahmat, Zaidah

doi:10.1016/j.rsci.2019.04.001

Cited by 24 publications

(21 citation statements)

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“…The previous studies showed that callus from tissue culture may induce variations (Bairu et al 2011), known as somaclonal variations that hamper the application of in vitro culture to develop transgenic rice plants. In in vitro culture, callus produced through an in vitro culture system, is a good starting material for genetic transformation (Carsono and Yoshida 2008;Sahoo et al 2011;Tran and Sanan-Mishra 2015;Mukhtar and Hasnain, 2018;Mohammed et al 2019). Our study shows that optimization of the growth hormones application, in this case, 2,4-D, and callus induction time can reduce the somaclonal variations in terms of high number of green plant (plantlet) regeneration.…”

Section: Discussionmentioning

confidence: 80%

Optimize 2,4-D concentration and callus induction time enhance callus proliferation and plant regeneration of three rice genotypes

et al. 2021

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Abstract. Carsono N, Juwendah E, Liberty, Sari S, Damayanti F, Rachmadi M. 2021. Optimize 2,4-D concentration and callus induction time enhance callus proliferation and plant regeneration of three rice genotypes. Biodiversitas 22: 2555-2560. The development of callus in the course of transgenic rice avoids the somaclonal variants. To obtain a high number of normal phenotypes and a low number of somaclonal variants requires an appropriate 2,4-D concentration. In this study, we obtained the best callus induction time and a high number of green plant regeneration for three responsive rice genotypes on different 2,4-D concentrations in NB5 medium. The mature seeds of rice embryos were used as explants. A completely randomized factorial design was applied with four levels of 2,4-D concentrations (0, 1, 3, and 5 ppm), two levels of induction time (one and two weeks), and three rice genotypes (cv. Fatmawati, Nipponbare, and Kitaake). The study revealed that there was no interaction effect among genotype, 2,4-D concentration, and callus induction time. Three rice genotypes performed best in callus proliferation and regeneration. One-week callus induction time showed higher callus growth capacity (CGC) as compared to two-week callus induction time. Shoot regeneration capacity (SRC) was independently affected by genotype as well as by callus induction time. The interaction effect between 2,4-D concentration and callus induction time was observed on plant regeneration capacity (PRC). Without the addition of 2,4-D and 1 ppm of 2,4-D, the green plant regeneration capacity (GRC) was comparatively higher. Addition of 2,4-D showed a significant effect, especially at the plant regeneration stage. We found that one-week callus induction was the best treatment for callus proliferation and plant regeneration. We recommend the use of one-week callus induction and 1 ppm of 2,4-D for rice callus proliferation (sub-culture) and subsequent plant regeneration.

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Section: Discussionmentioning

confidence: 80%

Optimize 2,4-D concentration and callus induction time enhance callus proliferation and plant regeneration of three rice genotypes

et al. 2021

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“…Here, the regeneration capacity depends on the biomolecule delivery method employed and whether a stable transformation is desired (i.e., constitutive or transient; Cunningham et al, 2018). Various biological, chemical, and physical methods are available to deliver genetic materials into plant cells, including the aforementioned AMT, viral-mediated transformation, polymer-mediated delivery (e.g., polyethylene glycol [PEG)]), particle bombardment (gene gun-mediated or biolistic transformation), and electroporation (Ahmed et al, 2018;Mohammed et al, 2019;Shin et al, 2019;Tian et al, 2019;Imai et al, 2020;Ozyigit, 2020). The features of the conventional transformation methods are summarized in Table 1.…”

Section: Conventional Plant Biomolecule Delivery Approaches and Their Limitationsmentioning

confidence: 99%

“…Despite more than three decades of development, plant transformation and regeneration remain a challenge in several crop plants. AMT has proven to be more efficient for dicots than monocots (Sood et al, 2011;Van Eck, 2018;Mohammed et al, 2019) and is limited to a specific plant-host range (Cunningham et al, 2018;Demirer et al, 2019a). For example, AMT efficiency tends to be highly variable (6-99%) depending on the variety and subspecies of rice (Mohammed et al, 2019).…”

Section: Conventional Plant Biomolecule Delivery Approaches and Their Limitationsmentioning

confidence: 99%

“…AMT has proven to be more efficient for dicots than monocots (Sood et al, 2011;Van Eck, 2018;Mohammed et al, 2019) and is limited to a specific plant-host range (Cunningham et al, 2018;Demirer et al, 2019a). For example, AMT efficiency tends to be highly variable (6-99%) depending on the variety and subspecies of rice (Mohammed et al, 2019). In general, monocots are considered recalcitrant for Agrobacterium tumefaciens-mediated transformation (Sood et al, 2011;Hiei et al, 2014;Hofmann, 2016;Mookkan et al, 2017).…”

Section: Conventional Plant Biomolecule Delivery Approaches and Their Limitationsmentioning

confidence: 99%

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Advantage of Nanotechnology-Based Genome Editing System and Its Application in Crop Improvement

et al. 2021

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Agriculture is an important source of human food. However, current agricultural practices need modernizing and strengthening to fulfill the increasing food requirements of the growing worldwide population. Genome editing (GE) technology has been used to produce plants with improved yields and nutritional value as well as with higher resilience to herbicides, insects, and diseases. Several GE tools have been developed recently, including clustered regularly interspaced short palindromic repeats (CRISPR) with nucleases, a customizable and successful method. The main steps of the GE process involve introducing transgenes or CRISPR into plants via specific gene delivery systems. However, GE tools have certain limitations, including time-consuming and complicated protocols, potential tissue damage, DNA incorporation in the host genome, and low transformation efficiency. To overcome these issues, nanotechnology has emerged as a groundbreaking and modern technique. Nanoparticle-mediated gene delivery is superior to conventional biomolecular approaches because it enhances the transformation efficiency for both temporal (transient) and permanent (stable) genetic modifications in various plant species. However, with the discoveries of various advanced technologies, certain challenges in developing a short-term breeding strategy in plants remain. Thus, in this review, nanobased delivery systems and plant genetic engineering challenges are discussed in detail. Moreover, we have suggested an effective method to hasten crop improvement programs by combining current technologies, such as speed breeding and CRISPR/Cas, with nanotechnology. The overall aim of this review is to provide a detailed overview of nanotechnology-based CRISPR techniques for plant transformation and suggest applications for possible crop enhancement.

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“…Like in other crops, biolistic and Agrobacterium-mediated soybean transformation methods have mainly been used to recover GE events ( Table 2). Agrobacterium-mediated soybean transformation combining organogenesis-based regeneration is developed for soybean transgenesis and the transformation efficiency (TE) in several protocols has been improved (Yamada et al, 2012;Hada et al, 2018;Yang et al, 2018), but it is still low (∼10%) compared to the high efficiency in rice (∼40%) (Mohammed et al, 2019). Cotyledons of soybean mature seeds were usually utilized as explants for regeneration of transformed cells.…”

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confidence: 99%

Progresses, Challenges, and Prospects of Genome Editing in Soybean (Glycine max)

Zhang

et al. 2020

Front. Plant Sci.

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Soybean is grown worldwide for oil and protein source as food, feed and industrial raw material for biofuel. Steady increase in soybean production in the past century mainly attributes to genetic mediation including hybridization, mutagenesis and transgenesis. However, genetic resource limitation and intricate social issues in use of transgenic technology impede soybean improvement to meet rapid increases in global demand for soybean products. New approaches in genomics and development of site-specific nucleases (SSNs) based genome editing technologies have expanded soybean genetic variations in its germplasm and have potential to make precise modification of genes controlling the important agronomic traits in an elite background. ZFNs, TALENS and CRISPR/Cas9 have been adapted in soybean improvement for targeted deletions, additions, replacements and corrections in the genome. The availability of reference genome assembly and genomic resources increases feasibility in using current genome editing technologies and their new development. This review summarizes the status of genome editing in soybean improvement and future directions in this field.

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Agrobacterium-Mediated Transformation of Rice: Constraints and Possible Solutions

Cited by 24 publications

References 119 publications

Optimize 2,4-D concentration and callus induction time enhance callus proliferation and plant regeneration of three rice genotypes

Optimize 2,4-D concentration and callus induction time enhance callus proliferation and plant regeneration of three rice genotypes

Advantage of Nanotechnology-Based Genome Editing System and Its Application in Crop Improvement

Progresses, Challenges, and Prospects of Genome Editing in Soybean (Glycine max)

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