Agrobacterium-mediated in planta transformation of maize via pistil filaments

Transgenics in groundnut (Arachis hypogaea L.) expressing cry1AcF gene for resistance to Spodoptera litura (F.)

“…K-134, transgenics were developed by a tissue culture independent Agrobacterium-mediated in planta transformation strategy. The in planta transformation protocols have been developed for transformation in many crop species, Arabidopsis thaliana (Feldmann and Marks 1987), soybean (Trick and Finer 1997), sunflower (Rao and Rohini 1999), safflower (Rohini and Rao 2000), rice (Supartana et al 2005), maize (Chumakov et al 2006) and cotton (Keshamma et al 2008). Earlier an in planta transformation protocol was developed in groundnut by Rohini and Rao (2000) and proved its reproducibility by Keshamma et al (2008).…”

Pollen grains as a target for introduction of foreign genes into plants: an assessment

“…Most of them lead to production of chimeras and somaclonal variation. Among the alternate methods for development of transgenic plants (Rakoczy-Trojanowska 2002), methods such as the floral dip method (Clough and Bent 1998), pollen transformation (Wang et al 2001), pistil transformation (Chumakov et al 2006;Mamontova et al 2010) and ovary-dip transformation (Yang et al 2009) have been found to be easier, avoiding the in vitro phase and requisite for regeneration. In planta methods such as floral dip method, although demonstrated in a few species such as Arabidopsis thaliana (Clough and Bent 1998), is yet to be extended to other species.…”

“…In planta transformation methods which include floral dip method (Clough and Bent 1998), pipetting Agrobacterium into developing spikelets (Hess et al 1990), pollen tube mediated gene transfer (Zhou et al 1983), pistil transformation (Chumakov et al 2006) and direct pollen transformation have been successful only in a few species. Tissue culture prerequisite…”

Agrobacterium tumefaciens-MEDIATED IN-PLANTA TRANSFORMATION OF INDONESIAN MAIZE USING pIG121Hm-Cs PLASMID CONTAINING nptII AND hpt GENES

“…Supartana et al (2005) obtained the levels of efficiency of in-planta transformation of 40% (PCR) and 34% (GUS histochemical test) by inoculation of embryonic apical meristem rice plants. In-planta transformation using filament pistil (female flowers) of maize with plasmid pTd33 containing gus and nptII genes showed that 6.8% sprouts from the seeds were resistant to kanamycin and 60.3% of the resistant gene nptII were positive (Chumakov et al 2006). Other study revealed that all tested genotypes of sugarcane cv.…”

“…The direct transformation method without any tissue culture steps is termed as in-planta transformation (Chumakov et al 2006). Floral dip method has been used in genetic engineering strategy as it directly produces genetically modified transformant without the laborious tissue culturing procedures (Martins et al 2015).…”