Pollen grains as a target for introduction of foreign genes into plants: an assessment

Eapen, Susan

doi:10.1007/s12298-010-0042-6

Cited by 27 publications

(21 citation statements)

References 69 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, pollen has been used for laser mediated transformation due to its ease of culture, but transfection efficiency was low [5]. Specifically, Lilum hybrid pollen walls were perforated with an energy density of 1.42 J/cm 2 at 0.6 ms from a near infrared (NIR) laser (Ti:Sapphire, 1kHz) and infiltrated with a mitochondrion-targeted red fluorescent protein construct [6].…”

Section: Introductionmentioning

confidence: 99%

Optoperforation of single, intact Arabidopsis cells for uptake of extracellular dye-conjugated dextran

LeBlanc¹,

Merritt²,

McMillan³

et al. 2013

Opt. Express

View full text Add to dashboard Cite

A plant science research goal is to manipulate single cells in an intact organism in order to study their interactions with neighboring cells. Based on a technique previously demonstrated in isolated plant cells, mammalian cells and cyanobacteria, Arabidopsis epidermal cells were optoperforated to allow for uptake of external cascade blue-labeled dextrans. Adverse organelle responses were determined to be minimal and dye retention was demonstrated for at least 72 hours. This technique overcomes the physical challenges presented by the plant cell wall and demonstrates the feasibility of in situ optoperforation.

show abstract

Section: Introductionmentioning

confidence: 99%

Optoperforation of single, intact Arabidopsis cells for uptake of extracellular dye-conjugated dextran

LeBlanc¹,

Merritt²,

McMillan³

et al. 2013

Opt. Express

View full text Add to dashboard Cite

show abstract

“…Many tissue culture and genetic transformation techniques have been developed to create genetically engineered plants that can tolerate environmental stress, and improve productivity and quality of plant species (Tu et al 2005, García-Sogo et al 2010, Eapen 2011, Alikina et al 2016Souza et al 2017). However, the efficiency of current gene transfer techniques are still low, judged by the difficulties in recovering fertile transgenic plants and time constraints (Saunders andMatthews 1995, Koetle et al 2017).…”

Section: Introductionmentioning

confidence: 99%

“…Pollen transformation using pollen as a "super vector" was proposed earlier (Hess 1987). However, a routine method for pollen transformation is still lacking (Loguercio et al 1994, Hudson et al 2001, Eapen 2011, Han et al 2015. Studies on selection of transformed pollen for the expression of genes are important in recombinant DNA technology, which is a major challenge for production of higher efficiency of transgenic plants.…”

Section: Introductionmentioning

confidence: 99%

Gene transfer utilizing pollen-tubes of Albuca nelsonii and Tulbaghia violacea

Kumari

Baskaran

Staden

2017

Crop Breed. Appl. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…Among the reproductive system transformation methods, the first proposed pollen-tube pathway (Zhou, 1983) showed a great success in cotton (Yang et al, 2007). Further, pollenmediated transformation was proposed based on the blooming characteristics of maize; therefore, the operation was observed to be more suitable for the transformation of plants, especially for maize, which produced large amounts of pollen (Eapen, 2011).…”

Section: Introductionmentioning

confidence: 99%

Development of Plant Expression Vector with Taq DNA Polymerase Gene to Yield Heat-tolerant Maize Lines

Zhang¹,

Zhou²,

Bai³

et al. 2017

IJAB

View full text Add to dashboard Cite

Thermus aquaticus (Taq) DNA polymerase has been extensively used in DNA amplification procedures, However, the application of Taq DNA polymerase gene in plant has not yet been reported. In this study, we have focused on using Taq DNA polymerase gene to develop heat-tolerant maize transgenic lines, which would aid in controlling crop loss due to heat stress and potentially increase the economic value. Firstly, an expression vector with Taq DNA polymerase gene was constructed and introduced into the maize ZhongKai inbred lines, Zhong sweet No. 1 and Zhong glutinous No. 2 by pollen-mediated transformation to obtain the transgenic maize plants. Then, the harvested T1 generation seeds were confirmed to be positive transformants by PCR and Southern blotting. The screening of the T2 generation by exposure to heat stress conditions in the field combined with physiological indexes resulted in nine heat-tolerant transgenic maize lines. The expression vector with Taq DNA polymerase gene, developed in the present study, will certainly expand more avenues for heat-tolerant genetic transformation in plants.

show abstract

Pollen grains as a target for introduction of foreign genes into plants: an assessment

Cited by 27 publications

References 69 publications

Optoperforation of single, intact Arabidopsis cells for uptake of extracellular dye-conjugated dextran

Optoperforation of single, intact Arabidopsis cells for uptake of extracellular dye-conjugated dextran

Gene transfer utilizing pollen-tubes of Albuca nelsonii and Tulbaghia violacea

Development of Plant Expression Vector with Taq DNA Polymerase Gene to Yield Heat-tolerant Maize Lines

Contact Info

Product

Resources

About