Agro-industrial oily wastes as substrates for PHA production by the new strain Pseudomonas aeruginosa NCIB 40045: Effect of culture conditions

Evaluation of bioremediation potential and biopolymer production of pseudomonads isolated from petroleum hydrocarbon-contaminated areas

“…Fernandez et al (2005 reported that residual waste frying and other oily wastes are suitable substrates for PHA production by P. aeruginosa 42A2, and they found that monomer composition of the PHA polymers varies with the substrate supplied. C 7 , C 9 , C 14:2 , and C 16:0 were described as a monomers of polymer (Fernandez et al 2005), and these studies support present results.…”

“…The concentration variations of crude oil as carbon source from 0.5 to 3 % v/v showed that with 2 % v/v crude oil, the highest biomass occurs and the greatest production of PHA was obtained, but when the concentration of crude oil rose above 2 % v/v, there was an inhibitory effect on bacterial growth and PHA production, and this inhibitory effect was ascribed to problems linked to the toxicity of crude oil (compounds such as sulfur compounds) and the difficulty to access to the nutrients in the culture medium. He et al (1998) described that P. stutzeri 1317 isolated from oil-contaminated soil was found to grow well in glucose and soybean oil as a sole carbon source, respectively, and synthesized PHA containing medium chain length monomers of hydroxyalkanoates ranging from C 6 to C 14 (He et al 1998). Fernandez et al (2005 reported that residual waste frying and other oily wastes are suitable substrates for PHA production by P. aeruginosa 42A2, and they found that monomer composition of the PHA polymers varies with the substrate supplied.…”

Plant Oils and Products of Their Hydrolysis as Substrates for Polyhydroxyalkanoate Synthesis

“…Furthermore, a comparative study of oils versus fatty acid mixtures is not currently available. Mcl-PHA has been accumulated by Pseudomonas aeruginosa using palm oil 11 , Brassica carinta oil 12 and waste frying oil 13 , however, this species is an opportunistic pathogen and not suitable for PHA production at an industrial level. Pseudomonas chlororaphis HS21 was previously used to produce mcl-PHA from palm oil 14 .…”

“…Surprisingly, none of these studies has examined the ability of the strains to grow to high cell density when cultivated on plant oils, growth has been carried out in shake flasks or to low cell density in batch fermentation. Some of the studies compared the growth on oils to growth on individual pure fatty acids, mainly oleic acid 11,12,13,14,16 , but there has been no comprehensive comparison between growth and PHA production using plant oils and crude fatty acid mixtures (generated from the hydrolysis of oils).…”

Fermentation of Sweet Sorghum into Added Value Biopolymer of Polyhydroxyalkanoates (PHAs)

“…Fig.7 shows PHA accumulated in their cells that are characterized by transmission electron microscopic (TEM) technique. (Fernandez et al, 2005), Bacillus mycoides (Thakur et al, 2001) and Escherichia coli, etc.…”