Plant Oils and Products of Their Hydrolysis as Substrates for Polyhydroxyalkanoate Synthesis

Walsh, Mark A.; O’Connor, Kevin E.; Babu, Ramesh; Woods, Trevor; Kenny, Shane T.

doi:10.15255/cabeq.2014.2252

Cited by 29 publications

(10 citation statements)

References 33 publications

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“…PHA accumulated by bacteria supplied with vegetable oil derived free fatty acids and animal fat derived free fatty acids display liquid properties at room temperature similar to the polymers arising from the supply of HWCOFAs to bacteria in the current study [21,56]. These polymer properties can be explained by the high degree of disorder and by the long chain monomers acting as internal plasticisers [30].…”

Section: Discussionsupporting

confidence: 58%

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High Cell Density Conversion of Hydrolysed Waste Cooking Oil Fatty Acids Into Medium Chain Length Polyhydroxyalkanoate Using Pseudomonas putida KT2440

Ruiz

Kenny

et al. 2019

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Waste cooking oil (WCO) is a major pollutant, primarily managed through incineration. The high cell density bioprocess developed here allows for better use of this valuable resource since it allows the conversion of WCO into biodegradable polymer polyhydroxyalkanoate (PHA). WCO was chemically hydrolysed to give rise to a mixture of fatty acids identical to the fatty acid composition of waste cooking oil. A feed strategy was developed to delay the stationary phase, and therefore achieve higher final biomass and biopolymer (PHA) productivity. In fed batch (pulse feeding) experiments Pseudomonas putida KT2440 achieved a PHA titre of 58 g/l (36.4% of CDW as PHA), a PHA volumetric productivity of 1.93 g/l/h, a cell density of 159.4 g/l, and a biomass yield of 0.76 g/g with hydrolysed waste cooking oil fatty acids (HWCOFA) as the sole substrate. This is up to 33-fold higher PHA productivity compared to previous reports using saponified palm oil. The polymer (PHA) was sticky and amorphous, most likely due to the long chain monomers acting as internal plasticisers. High cell density cultivation is essential for the majority of industrial processes, and this bioprocess represents an excellent basis for the industrial conversion of WCO into PHA.

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Section: Discussionsupporting

confidence: 58%

“…High production costs, compared to the traditional petrol-based plastics, remain the major challenge for polyhydroxyalkanoates entry into the plastics market. The use of inexpensive carbon sources, such as waste products, and a highly productive fermentation process could help to overcome the production costs [21][22][23][24].…”

Section: Introductionmentioning

confidence: 99%

High Cell Density Conversion of Hydrolysed Waste Cooking Oil Fatty Acids Into Medium Chain Length Polyhydroxyalkanoate Using Pseudomonas putida KT2440

Ruiz

Kenny

et al. 2019

Catalysts

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“…Plant oils contain higher carbon content per weight than sugars, suggesting a PHA yield of at least two times higher 15 . They are catabolized via β-oxidation cycle to produce polymers of different chain lengths 12 .…”

Section: Introductionmentioning

confidence: 99%

Impact of Different Bacterial Strains on the Production, Composition, and Properties of Novel Polyhydroxyalkanoates Using Crude Palm Oil as Substrate

Rodrigues¹

2018

Chem.Biochem.Eng.Q.

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Polyhydroxyalkanoates (PHAs) are a group of biodegradable polymers produced from renewable sources by prokaryotic biocatalysts, accumulated intracellularly for energy and carbon storage. In the present study, production and characterization of PHAs synthetized by Cupriavidus necator (IPT 026 and IPT 027) and Burkholderia cepacia (IPT 119 and IPT 400) were evaluated using crude palm oil (C 16:0 = 26.44 %, C 18:1 = 54.50 %, C 18:2 = 13.41 %) as substrate (15 g L-1 crude palm oil, pH 7.0, 180 rpm, 72 h). All strains were able to synthesize novel PHA copolymers (0.10-1.45 g L-1), and IPT 027 displayed the highest production. Copolymers monomeric composition (M w = 173.78-389.30 kDa) was comprised mostly of hydroxyhexadecanoate (41.43-53.15 %) and hydroxy-9-octadecenoate (14.91-29.61 %). PHAs were predominantly amorphous, showed low polydispersity, and good thermal stability (T onset ≥ 283 °C), which increased proportionally to crystallinity. Crude palm oil constitutes an emerging alternative for PHAs production, and microorganism strains strongly affect polymer accumulation, monomeric composition, molar mass, and properties.

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“…In this context, there is an increasing trend towards the application of carbon-rich (agro) industrial waste materials to produce the so called “2nd generation PHA” [ 4 ]. Among these materials, the current literature familiarizes us with PHA production based on surplus whey [ 27 ], abundant lignocelluloses [ 28 , 29 , 30 ], waste lipids from animal processing [ 31 , 32 , 33 ], used plant-and cooking oils [ 34 , 35 , 36 ], crude glycerol from biodiesel production [ 37 , 38 , 39 , 40 ], plant root hydrolysates [ 30 ], extracts and hydrolysates of spent coffee ground [ 41 , 42 ], and molasses [ 43 ]. Such waste materials already performed well as substrates on the laboratory scale, but are still awaiting their implementation in industrial-scale PHA production processes.…”

Section: Introductionmentioning

confidence: 99%

Fed-Batch Synthesis of Poly(3-Hydroxybutyrate) and Poly(3-Hydroxybutyrate-co-4-Hydroxybutyrate) from Sucrose and 4-Hydroxybutyrate Precursors by Burkholderia sacchari Strain DSM 17165

et al. 2017

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Based on direct sucrose conversion, the bacterium Burkholderia sacchari is an excellent producer of the microbial homopolyester poly(3-hydroxybutyrate) (PHB). Restrictions of the strain’s wild type in metabolizing structurally related 3-hydroxyvalerate (3HV) precursors towards 3HV-containing polyhydroxyalkanoate (PHA) copolyester calls for alternatives. We demonstrate the highly productive biosynthesis of PHA copolyesters consisting of 3-hydroxybuytrate (3HB) and 4-hydroxybutyrate (4HB) monomers. Controlled bioreactor cultivations were carried out using saccharose from the Brazilian sugarcane industry as the main carbon source, with and without co-feeding with the 4HB-related precursor γ-butyrolactone (GBL). Without GBL co-feeding, the homopolyester PHB was produced at a volumetric productivity of 1.29 g/(L·h), a mass fraction of 0.52 g PHB per g biomass, and a final PHB concentration of 36.5 g/L; the maximum specific growth rate µmax amounted to 0.15 1/h. Adding GBL, we obtained 3HB and 4HB monomers in the polyester at a volumetric productivity of 1.87 g/(L·h), a mass fraction of 0.72 g PHA per g biomass, a final PHA concentration of 53.7 g/L, and a µmax of 0.18 1/h. Thermoanalysis revealed improved material properties of the second polyester in terms of reduced melting temperature Tm (161 °C vs. 178 °C) and decreased degree of crystallinity Xc (24% vs. 71%), indicating its enhanced suitability for polymer processing.

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Plant Oils and Products of Their Hydrolysis as Substrates for Polyhydroxyalkanoate Synthesis

Cited by 29 publications

References 33 publications

High Cell Density Conversion of Hydrolysed Waste Cooking Oil Fatty Acids Into Medium Chain Length Polyhydroxyalkanoate Using Pseudomonas putida KT2440

High Cell Density Conversion of Hydrolysed Waste Cooking Oil Fatty Acids Into Medium Chain Length Polyhydroxyalkanoate Using Pseudomonas putida KT2440

Impact of Different Bacterial Strains on the Production, Composition, and Properties of Novel Polyhydroxyalkanoates Using Crude Palm Oil as Substrate

Fed-Batch Synthesis of Poly(3-Hydroxybutyrate) and Poly(3-Hydroxybutyrate-co-4-Hydroxybutyrate) from Sucrose and 4-Hydroxybutyrate Precursors by Burkholderia sacchari Strain DSM 17165

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