High Cell Density Conversion of Hydrolysed Waste Cooking Oil Fatty Acids Into Medium Chain Length Polyhydroxyalkanoate Using Pseudomonas putida KT2440

Ruiz, Carolina; Kenny, Shane T.; P, Ramesh Babu; Walsh, Mark A.; Narancic, Tanja; O’Connor, Kevin E.

doi:10.3390/catal9050468

Cited by 31 publications

(22 citation statements)

References 59 publications

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“…Kamilah and co-workers have also shown that wild type and engineered strains of C. necator were able to utilize WCO (palm oil) in the production of P(3HB) and P(3HB-co-3HHx), respectively (Kamilah et al, 2013(Kamilah et al, , 2018. In a separate study, it was reported that P. putida strain KT2440 was able to produce high contents of mcl-PHAs when grown via high cell density fermentation using hydrolyzed WCO as the sole carbon source (Ruiz et al, 2019). Kourmentza et al, on the other hand, have reported co-production of P(3HB) polymers along with rhamnolipids when Burkholderia thailandensis was employed for the bioconversion of used cooking oil.…”

Section: Biosynthesis Of Phas Using Waste Plant Oilsmentioning

confidence: 99%

Can Polyhydroxyalkanoates Be Produced Efficiently From Waste Plant and Animal Oils?

Surendran

Lakshmanan

Chee

et al. 2020

Front. Bioeng. Biotechnol.

111

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Polyhydroxyalkanoates (PHAs) are a potential replacement for some petrochemical-based plastics. PHAs are polyesters synthesized and stored by various bacteria and archaea in their cytoplasm as water-insoluble inclusions. PHAs are usually produced when the microbes are cultured with nutrient-limiting concentrations of nitrogen, phosphorus, sulfur, or oxygen and excess carbon sources. Such fermentation conditions have been optimized by industry to reduce the cost of PHAs produced commercially. Industrially, these biodegradable polyesters are derived from microbial fermentation processes utilizing various carbon sources. One of the major constraints in scaling-up PHA production is the cost of the carbon source metabolized by the microorganisms. Hence, cheap and renewable carbon substrates are currently being investigated around the globe. Plant and animal oils have been demonstrated to be excellent carbon sources for high yield production of PHAs. Waste streams from oil mills or the used oils, which are even cheaper, are also used. This approach not only reduces the production cost for PHAs, but also makes a significant contribution toward the reduction of environmental pollution caused by the used oil. Advancements in the genetic and metabolic engineering of bacterial strains have enabled a more efficient utilization of various carbon sources, in achieving high PHA yields with specified monomer compositions. This review discusses recent developments in the biosynthesis and classification of various forms of PHAs produced using crude and waste oils from the oil palm and fish industries. The biodegradability of the PHAs produced from these oils will also be discussed.

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Section: Biosynthesis Of Phas Using Waste Plant Oilsmentioning

confidence: 99%

Can Polyhydroxyalkanoates Be Produced Efficiently From Waste Plant and Animal Oils?

Surendran

Lakshmanan

Chee

et al. 2020

Front. Bioeng. Biotechnol.

111

View full text Add to dashboard Cite

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“…The reason behind this is a genetic makeup that leads to the metabolism of a specific carbon feed (Ashby and Foglia, 1998). Ruiz et al (2019) studied the effect of hydrolyzed waste cooking oil fatty acids (HWCOFA) that showed HWCOFA could be used as the sole substrate for PHA production by P. putida KT2440. Glucose was also a suitable substrate for PHA production (Mohapatra et al, 2015).…”

Section: Influence Of Physical Parameters On Pha Productionmentioning

confidence: 99%

Optimization of Growth Conditions to Produce Sustainable Polyhydroxyalkanoate Bioplastic by Pseudomonas aeruginosa EO1

2021

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Polyhydroxyalkanoates (PHAs) are intracellularly synthesized by bacteria as carbonosomes that exhibit biodegradable thermoplastics and elastomeric properties. The use of cheaper edible oils as a source of carbon assists in the reduction of the production cost of such biopolyesters. In this work, different edible oils, such as groundnut oil (GNO), mustard oil, sesame oil, and soybean oil (SBO) were used to check their effect on PHA production from Pseudomonas aeruginosa EO1 (MK049902). Pseudomonas aeruginosa EO1 was used in a two-stage production system. In the first stage, bacterial growth was favored and, in the second, PHA was synthesized. GNO was found as the best carbon source for PHA production. The use of 2% (v/v) GNO, rich in saturated fatty acids, allowed PHA content of 58.41% and dry cell weight (DCW) of 10.5g/L at pH7 and temperature 35°C for 72h. Groundnut has a high potential for oil production and for the diversification of co-products with some potential of value aggregation. Such a perennial and sustainable species will almost certainly meet the criteria for becoming a significant commercial oilseed crop. Fourier transform infrared spectroscopy (FTIR) spectra showed strong characteristic bands at 1,282, 1,725, 2,935, 2,999, and 3,137cm−1 for the PHA polymer. Gas chromatography-mass spectrometry (GC-MS) detects the presence of PHA copolymers.

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“…The implemented feed strategy delayed the stationary phase to enhance biomass and PHA formation. Use of intermittent feeding and conditions that led to HCDC, P. putida KT2440 reached a total biomass, %-mcl-PHA content in the CDW and overall productivity of 159.4 g/L, 36.4% (i.e., 57 g/L mcl-PHA) and 1.93 g/L/h, respectively [ 250 ]. This bioprocess provides a strong foundation that, with further process optimization, could lead to a commercially viable process for conversion of WCO to PHA.…”

Section: Industrial/agro-industrial Waste For Production Of Scl-and Mcl-phamentioning

confidence: 99%

Emergent Approaches to Efficient and Sustainable Polyhydroxyalkanoate Production

2021

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Petroleum-derived plastics dominate currently used plastic materials. These plastics are derived from finite fossil carbon sources and were not designed for recycling or biodegradation. With the ever-increasing quantities of plastic wastes entering landfills and polluting our environment, there is an urgent need for fundamental change. One component to that change is developing cost-effective plastics derived from readily renewable resources that offer chemical or biological recycling and can be designed to have properties that not only allow the replacement of current plastics but also offer new application opportunities. Polyhydroxyalkanoates (PHAs) remain a promising candidate for commodity bioplastic production, despite the many decades of efforts by academicians and industrial scientists that have not yet achieved that goal. This article focuses on defining obstacles and solutions to overcome cost-performance metrics that are not sufficiently competitive with current commodity thermoplastics. To that end, this review describes various process innovations that build on fed-batch and semi-continuous modes of operation as well as methods that lead to high cell density cultivations. Also, we discuss work to move from costly to lower cost substrates such as lignocellulose-derived hydrolysates, metabolic engineering of organisms that provide higher substrate conversion rates, the potential of halophiles to provide low-cost platforms in non-sterile environments for PHA formation, and work that uses mixed culture strategies to overcome obstacles of using waste substrates. We also describe historical problems and potential solutions to downstream processing for PHA isolation that, along with feedstock costs, have been an Achilles heel towards the realization of cost-efficient processes. Finally, future directions for efficient PHA production and relevant structural variations are discussed.

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High Cell Density Conversion of Hydrolysed Waste Cooking Oil Fatty Acids Into Medium Chain Length Polyhydroxyalkanoate Using Pseudomonas putida KT2440

Cited by 31 publications

References 59 publications

Can Polyhydroxyalkanoates Be Produced Efficiently From Waste Plant and Animal Oils?

Can Polyhydroxyalkanoates Be Produced Efficiently From Waste Plant and Animal Oils?

Optimization of Growth Conditions to Produce Sustainable Polyhydroxyalkanoate Bioplastic by Pseudomonas aeruginosa EO1

Emergent Approaches to Efficient and Sustainable Polyhydroxyalkanoate Production

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