Evaluation of bioremediation potential and biopolymer production of pseudomonads isolated from petroleum hydrocarbon-contaminated areas

Goudarztalejerdi, Ali; Tabatabaei, Mohammad; Eskandari, Mohammad Hadi; Mowla, Dariush; Iraji, Aida

doi:10.1007/s13762-015-0779-0

Cited by 24 publications

(24 citation statements)

References 36 publications

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“…It is well known that produced water has high quantities of dissolved crude oil, petroleum and related hydrocarbons (1). This carbon rich composition also makes it an ideal environment for many polyhydroxyalkanoates producing bacterial species since PHA inclusion bodies are produced as energy reserves in the presence of high carbon content (2,59). In the current study, isolation of PHA producing bacteria from produced water presents significant two-fold results in environmental studies (60).…”

Section: Discussionmentioning

confidence: 99%

Animal fat and glycerol bioconversion to polyhydroxyalkanoate by produced water bacteria

et al. 2020

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AbstractOil reservoirs contain large amounts of hydrocarbon rich produced water, trapped in underground channels. Focus of this study was isolation of PHA producers from produced water concomitant with optimization of production using animal fat and glycerol as carbon source. Bacterial strains were identified as Bacillus subtilis (PWA), Pseudomonas aeruginosa (PWC), Bacillus tequilensis (PWF), and Bacillus safensis (PWG) based on 16S rRNA gene sequencing. Similar amounts of PHA were obtained using animal fat and glycerol in comparison to glucose. After 24 h, high PHA production on glycerol and animal fat was shown by strain PWC (5.2 g/ L, 6.9 g/ L) and strain PWF (12.4 g/ L, 14.2 g/ L) among all test strains. FTIR analysis of PHA showed 3-hydroxybutyrate units. The capability to produce PHA in the strains was corroborated by PhaC synthase gene sequencing. Focus of future studies can be the use of lipids and glycerol on industrial scale.

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Section: Discussionmentioning

confidence: 99%

Animal fat and glycerol bioconversion to polyhydroxyalkanoate by produced water bacteria

et al. 2020

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“…Nile blue A is a fluorescent dye that stains PHA granules in bacterial cells and gives a characteristic orange glow under UV light of 365 nm. The intensity of the glow is directly proportional to the concentration of PHA accumulation in the cells (Goudarztalejerdi et al, 2015). Ten bacterial isolates from 80 colonies showed fluorescence of different intensities.…”

Section: Isolation and Identification Of Pha Producing Bacteriamentioning

confidence: 99%

“…The numbers at the node indicate bootstrap values as percentages obtained with 1000 resampling analyses. Goudarztalejerdi et al (2015).…”

Section: Isolate Growth and Optimization Of Pha Accumulationmentioning

confidence: 99%

“…Soil contamination with petroleum and petroleumbased hydrocarbons result to environmental hazards and health defects (Goudarztalejerdi et al, 2015). Bioremediation methods have received positive publicity because they promise to be environmentally friendly, efficient and cheap treatment technologies for the remediation of hydrocarbon contamination.…”

Section: Introductionmentioning

confidence: 99%

“…Some microorganisms have been reported to degrade crude oil and accumulate PHA in limited nutrients (Goudarztalejerdi et al, 2015). The use of such microorganisms for PHA production would provide several advantages, namely, reduction in the cost of producing PHAs, value addition to waste petroleum byproducts, bioremediation and recovery of biodegradable plastics from the cell biomass.…”

Section: Introductionmentioning

confidence: 99%

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Production of polyhydroxyalkanoates by hydrocarbonaclastic bacteria

Mwamburi¹,

Betty²,

Kasili³

et al. 2019

Afr. J. Biotechnol.

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Production of polyhydroxyalkanoates (PHA) by bacteria isolated from spent engine oil (SEO) contaminated soils was investigated using nitrogen limitation in the production medium. Out of ten isolates, three were selected as the best based on their ability to metabolize SEO effectively and fluoresce orange when stained with Nile Blue A dye. Fourier Transform Infrared spectrophometer was used as a confirmatory test for PHA detection by tracking the carbonyl bands on the spectra. Optimum growth of the isolates occurred at 1% (v/v) SEO, pH 7, 37°C for K1+VE and Kar5+VE1 while for Kar5+VE2 was at 1% (v/v) SEO, PH 7, 40°C and 150 RPM. Sequencing of 16S rDNA partial genes grouped the isolates into 6 different genera: Ochrobactrum, Pseudoxanthomonas, Bodetella, Achromobacter, Alcaligenes and Acinetobacter species. Isolate K1+VE, identified as an Ochrobactrum produced Poly (3hydroxyheptanoate) 46% (w/w), isolate Kar5+VE1, identified as Achromobacter spp. produced poly (3hydroxybutyrate) 20% (w/w)) while isolate Kar5+VE2, identified as an Alcaligenes spp. produced a copolymer poly-3-(3-hydroxybutyrate-Co-3-hydroxyoctanoate) 45% (w/w). Orthoxylene and ethylbenzene were the major hydrocarbons in spent engine oil before degradation while ethylhexanol was the major degradation product as identified by Gas Chromatography-Mass Spectrophotometry. The isolates were able to degrade hydrocarbons as well as produce polyhydroxyalkanoates.

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Oil spill bioremediation strategies in Brazilian tropical seawater—The role of polycyclic aromatic hydrocarbons degradation

de Souza,

Becker,

Vieira‐da‐Motta

et al. 2023

CLEAN Soil Air Water

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For this research, a 28‐day bioremediation experiment was conducted, simulating an oil spill on the Brazilian coast using mesocosm units and two different bioremediation strategies, adding rate‐limiting nutrients in the form of NPK fertilizer during the first four days (Bior1) and weekly (Bior2). The gas chromatography–mass spectrometry analysis results showed that oil natural degradation (Control) removed 33.8 wt.% of the alkanes on Day 7, while bioremediation processes plus evaporation in Bior1 and Bior2 contributed to minimizing alkanes in 83.4 and 80.8 wt.%, respectively. Bior1 strategy also accelerated the biodegradation of recalcitrant polycyclic aromatic hydrocarbons (PAH) such as phenanthrene, methyl phenanthrenes, and methyl dibenzothiophenes up to the second week when compared to Bior2. Low‐cost NPK fertilizer addition demonstrated efficiency in the bioremediation strategy, especially in the first days of the simulated oil spill (Bior1), leading to savings in time and financial investment in recovering an oil contaminated area. Results also showed the efficiency of the oil‐degrading bacteria Pseudomonas mesophilica, identified for the first time in Brazilian seawater. In addition, results confirmed the influence of tropical temperatures over oil natural natural degradation and bioremediation as an oil spill cleanup solution for recovering contaminated areas in tropical countries, especially in sensitive marine environments.

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Evaluation of bioremediation potential and biopolymer production of pseudomonads isolated from petroleum hydrocarbon-contaminated areas

Cited by 24 publications

References 36 publications

Animal fat and glycerol bioconversion to polyhydroxyalkanoate by produced water bacteria

Animal fat and glycerol bioconversion to polyhydroxyalkanoate by produced water bacteria

Production of polyhydroxyalkanoates by hydrocarbonaclastic bacteria

Oil spill bioremediation strategies in Brazilian tropical seawater—The role of polycyclic aromatic hydrocarbons degradation

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