Animal fat and glycerol bioconversion to polyhydroxyalkanoate by produced water bacteria

Sohail, Rafeya; Jamil, Nazia; Ali, Iftikhar; Munir, Sajida

doi:10.1515/epoly-2020-0011

Cited by 12 publications

(5 citation statements)

References 60 publications

(71 reference statements)

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“…Polyhydroxyalkanoate (PHA) was produced using previously isolated PHA producers (Sohail and Jamil 2019;Sohail et al 2020).Polylactide (PLA) was produced using corn starch and solvent cast PHA-PLA films were obtained after solvent induced recrystallization. PLA-PHA blends showed improved elasticity since blending with PHA enhances crystallinity of PLA.…”

Section: Discussionmentioning

confidence: 99%

Characterization and Degradation of Polyhydroxyalkanoates (PHA), Polylactides (PLA) and PHA-PLA Blends

Sohail¹,

Jamil²

2020

Preprint

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Biodegradable biopolymers such as polyhydroxyalkanoates (PHA) and polylactide (PLA) have wide range of applications in almost all sectors. Degradation of these polymers, however efficient, still creates a paradox with green chemistry principles. Blending of these polymers can potentially decrease plastic pollution due to their increased biodegradability. During this study, PHAwas produced using bacterial strains DL3; Bacillus subtilis (MT043898), PWA; Bacillus subtilis (MH142143), PWC; Pseudomonas aeruginosa (MH142144), PWF; Bacillus tequilensis (MH142145) and PWG; Bacillus safensis (MH142146). Corn-based PLA was produced chemically and physically blendedwith PHA (PHA-PLA blends). During molecular studies, PHA, PLA and PHA-PLA blendswere characterized via FT-IR analysis, SEM, and light microscopy indicating successful blending of PHA-PLA samples. FTIR results indicated PHA produced by strain PWF and mixed culture wasmcl-PHA. Degradation of polymers and copolymer blends was studied by bacteria for 12 weeks and in different environmental systems i.e. in soil, water, air, and heat for 20 weeks. Degradation analysis indicated highest degradation of PLA, followed by PHA-PLA blends. Among these systems, degradation was favored in soil (80%), followed by water (78%) and air (78%). Least degradation (76%) was observed on heat exposure. Sample degradation observed using light microscope and SEM showed fungal and bacterial colonization amidst cracks, crevices, bumps, and fractures after 20 weeks. Understanding the role of ambient microbial population during degradation and its impact on natural soil environment can be the focus of future studies.

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Section: Discussionmentioning

confidence: 99%

Characterization and Degradation of Polyhydroxyalkanoates (PHA), Polylactides (PLA) and PHA-PLA Blends

Sohail¹,

Jamil²

2020

Preprint

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“…A selective stain, Nile blue A, fluorescent dye is used for the detection of PHB granules [ 17 ]. Dye at 0.5μg/ml of selective media was composed and sterilized.…”

Section: Methodsmentioning

confidence: 99%

Selective isolation and genomic characterization of biopolymer producer—a novel feature of halophile Brachybacterium paraconglomeratum MTCC 13074

Mandragutti

Gódi

2023

Journal of Genetic Engineering and Biotechnology

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Background Biopolymers like polyhydroxyalkanoates (PHA) are the best natural macromolecules to use as alternative to the synthetic polymers. Many prokaryotes accumulate PHA as cytoplasmic intracellular granules and their accumulation is triggered by starving conditions. The PHAs are ecofriendly and used to create biodegradable plastics. The microbial synthesized PHA had acquired global importance in industrial and biomedical sectors. Results Ten different bacterial strains were isolated for the screening of PHA producers from the estuarine region of the Bay of Bengal, Suryalanka in Bapatla. A yellowish slimy circular colony known as M4 is actively growing on selective minimal media and was screened for polymeric granules in its cytoplasm using Sudan Black B and confirmed with the fluorescent dye Nile blue A. All of the isolates were biochemically tested and isolate M4 is the most capable of growing at high NaCl concentrations (3.2 percent) and tests positive for catalase, methyl red. The M4 strain revealed clear hydrolysis of gelatin, starch, and casein. The 16S rRNA sequencing revealed that M4 is 99.72% of identity to Brachybacterium paraconglomeratum LMG 19861(T) in BLAST and the obtained strain was assigned with accession no. MTCC 13074 and deposited in NCBI with accession no. MW899045. The chief cellular fatty acids found in M4 were C14:0, C15:0, C16:0, C18:1cis-9, C18:0, iso-C15: 0, iso-C14: 0, anteiso-C17: 0 and C18:1-7. Crotonic acid formation from M4-PHB extract was detected at 235nm in a UV spectrophotometer. Methanolysis was done, and derivatives of polyhydroxybutyric acid (PHB) in the extract were analyzed using GC-MS. Increasing viscosity was seen in the extracts which confirms the presence of polymer in the extracts. Thermogravimetric analysis was studied to determine the thermal profile of the PHB in the extract of M4. Conclusion In the study, the selective screening and extraction of ecofriendly PHB from M4 strain was highlighted. Brachybacterium paraconglomeratum is a novel strain showed its uniqueness by producing few monomeric derivatives of PHB. The strain was reporting for the first time as PHA producer. B. paraconglomeratum has promising characteristics according to its metabolic profile. In addition, this study also helps to understand the diversity of bacteria isolated from marine sources.

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“…For best confirmation, the isolates that showed a positive result with Sudan Black B stain were re-stained with alcoholic Nile Blue A stain (1%), and the plates were subjected to 365 wavelengths of ultraviolet light. The plates with blue fluorescent showed a positive result (Sohail et al, 2020).…”

Section: Screening For Phb -Producing Microorganismsmentioning

confidence: 98%

Isolation and identification of Polyhydroxybutyrate (PHB)-producing microorganisms from various sources in North Sinai

Shokr

Abd-Elsalam

Hamedo

et al. 2023

Catrina: The International Journal of Environmental Sciences

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The amount of non-biodegradable plastic waste on our planet is enormous. Natural materials like biomaterials are among the finest replacements for manmade plastics. In order to assist microorganisms resist severe environments, certain microbes generate bioplastics, which are lipid polyesters that build up as storage materials. The primary goal of this investigation was to isolate and characterize cultivable bacteria and fungi capable of producing bioplastics from North Sinai from different sources, such as saline soil, olive pomace, landfills, and seawater. Seven bacterial and five fungal isolates were selected from a total of 108 isolates to assay for PHB production and the selected isolates were stained with Sudan Black B for PHB formation, while Nile Blue A staining was used to detect the presence of PHA granules. All promising bacterial isolates with the highest PHB accumulation were identified as Halomonas, Lysinibacillus, Mesobacillus, Paracoccus, Paraliobacillus, Glutamicibacter, and Aquamicrobium; most fungal isolates were yeasts, identified as Rhodotorula, Hortaea, Meyerozyma, and Sarocladium by morphological and biochemical characterization and confirmed by molecular techniques.

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Animal fat and glycerol bioconversion to polyhydroxyalkanoate by produced water bacteria

Cited by 12 publications

References 60 publications

Characterization and Degradation of Polyhydroxyalkanoates (PHA), Polylactides (PLA) and PHA-PLA Blends

Characterization and Degradation of Polyhydroxyalkanoates (PHA), Polylactides (PLA) and PHA-PLA Blends

Selective isolation and genomic characterization of biopolymer producer—a novel feature of halophile Brachybacterium paraconglomeratum MTCC 13074

Isolation and identification of Polyhydroxybutyrate (PHB)-producing microorganisms from various sources in North Sinai

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