Isolation and identification of Polyhydroxybutyrate (PHB)-producing microorganisms from various sources in North Sinai

Abstract

The amount of non-biodegradable plastic waste on our planet is enormous. Natural materials like biomaterials are among the finest replacements for manmade plastics. In order to assist microorganisms resist severe environments, certain microbes generate bioplastics, which are lipid polyesters that build up as storage materials. The primary goal of this investigation was to isolate and characterize cultivable bacteria and fungi capable of producing bioplastics from North Sinai from different sources, such as saline soil, olive pomace, landfills, and seawater. Seven bacterial and five fungal isolates were selected from a total of 108 isolates to assay for PHB production and the selected isolates were stained with Sudan Black B for PHB formation, while Nile Blue A staining was used to detect the presence of PHA granules. All promising bacterial isolates with the highest PHB accumulation were identified as Halomonas, Lysinibacillus, Mesobacillus, Paracoccus, Paraliobacillus, Glutamicibacter, and Aquamicrobium; most fungal isolates were yeasts, identified as Rhodotorula, Hortaea, Meyerozyma, and Sarocladium by morphological and biochemical characterization and confirmed by molecular techniques.

Sustainable Synthesis of Biopolymer Polyhydroxybutyrate (PHB) from Agro-residue by Brevibacterium casei with Emphasis on Degradation Analysis

“…These positive isolates were characterized by their dark bluish-black appearance when subjected to the Sudan black B staining technique. The study conducted by Shokr et al, 28 states that, among a total of 58 fungal isolates, five isolates (specifically, B1, B11, E1, D6, and N8) demonstrated the capability to accumulate PHB when observed through the application of Sudan Black B staining technique. Additionally, among 50 bacterial isolates examined in the same study,…”

Antibacterial potential of essential oils extracted from three medicinal plants against some foodborne bacteria

“…PCR cycling conditions were as follows: one cycle of initial denaturation stage at 95 ºC for 3 min, followed by 35 amplification cycles [Denaturation at 95ºC for 30 s, annealing at 50 ºC for 30 s, extension at 72 ºC for 90 s], then a final extension step at 72 ºC for 5 min. PCR-DNA products (5µl) were visualized on ethidium bromide-stained 1% agarose gel (w/v) in Tris-acetate EDTA (TAE) buffer by a gel electrophoresis system (Shokr et al, 2023). Electrophoresis was performed at a constant 80V for 30min.…”