AgNOR polymorphism association with squamous intraepithelial lesions and invasive carcinoma with HPV infection

Alarcón‐Romero, Luz del Carmen; Illades‐Aguiar, Berenice; Flores‐Alfaro, Eugenia; Terán-Porcayo, Marco Antonio; Antonio-Véjar, Verónica; Reyes‐Maldonado, Elba

doi:10.1590/s0036-36342009000200009

Cited by 13 publications

(11 citation statements)

References 26 publications

(28 reference statements)

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“…This can be useful as a prognosis for the progression of squamous cell carcinoma. The present study which was done on precancerous and cancerous lesions of the cervix, similarly showed that the AgNOR was valuable as a cellular activity marker and as well as a marker of malignancy [11].…”

supporting

confidence: 61%

A Study on the Nucleolar Organizer Regions-As a Tumour Proliferative Marker in Cervical Smears

Shukla

Rajput²,

Acharya³

et al. 2013

JCDR

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Aim: To study the Argyrophilic Nucleolar Organizer Regions (AgNOR) as a cell proliferative marker in cervical smears and to assess its counts with the severity of the lesions on Papanicolaou (PAP) stained cervical smears. Material and Method:The present study was a prospective experimental study which was carried out at Jawaharlal Nehru Medical College in a span of 2 years. 50 high risk cases were selected from the Gynaecological OPD. The cervical smears were collected in pairs, one for the conventional PAP staining and the other one for the specialized AgNOR staining.Statistical Analysis: The Student's unpaired 't' test was used.

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supporting

confidence: 61%

A Study on the Nucleolar Organizer Regions-As a Tumour Proliferative Marker in Cervical Smears

Shukla

Rajput²,

Acharya³

et al. 2013

JCDR

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“…In our study, no statistical difference between the variation of AgNOR dots size and dispersion between dysplastic versus the SCC group was found. Alarcón‐Romero Ldel et al 21 . evaluated the relationships between AgNOR polymorphisms, squamous intraepithelial lesions and SCC with human papillomavirus infection.…”

Section: Discussionmentioning

confidence: 99%

“…The pattern of staining was classified according to the polymorphism of AgNOR dots; typical (spherical) and atypical (large, kidney‐shaped and clustered). They found that AgNOR polymorphism rose progressively according to the grade of histological lesions and therefore this marker was useful in the prognosis of SCC 21 …”

Section: Discussionmentioning

confidence: 99%

Comparison of Ki67 and modified AgNOR in dysplastic epithelial lesion, primary squamous cell carcinoma and metastatic squamous cell carcinoma of head and neck

Ashraf¹,

Azarpira²,

Safarzadegan³

et al. 2010

Basic and Applied Pathology

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The variations in nucleolar organizer regions (AgNOR) and the increase in Ki67 expression and correlation with histological findings in 64 specimen squamous epithelium from normal epithelium to overt carcinoma were evaluated. Mean AgNOR counts (mAgNOR), proliferative index (pAgNOR), variation in size and dispersion of AgNOR dots in cells were graded and compared in normal, dysplastic, primary and metastatic squamous cell carcinoma (SCC). The Ki67 percentage is significantly increased from normal squamous to SCC group, and the reactivity of staining were related to histological differentiation. The mAgNOR counts were high in all 44 cases of primary and metastatic SCC and low in normal squamous tissue and increased in dysplastic lesions. The mAgNOR count in SCC increased from well differentiated to poorly differentiated. PAgNOR counts were gradually increased in different grade of SCC, 1.4% in well differentiated, 6% in moderately differentiated, 19% in poorly differentiated. There was only significant difference between well differentiated versus poorly differentiated cases. Only significant correlation between Ki67 percentage and mAgNOR and between Ki67 percentage and pAgNOR in primary SCC group was noticed. Therefore, Ki67 is gold standard method for evaluation of proliferation activity. AgNOR quantity is proportional to the proliferative activity of the cell but does not necessarily indicate malignancy.

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“…They have found the pleomorphism of AgNOR increasing with progression of histopathological lesions, and the highest number was seen in squamous cell carcinoma [8–10]. The study assumes importance with the report of Alarcón-Romero et al that a significant difference was found with different types of AgNOR dots with infection of high-risk HPV types [10].…”

Section: Discussionmentioning

confidence: 83%

“…This has become imperative in view of the findings of Alarcón-Romero et al that the pleomorphic dots are related to the HPV infection [10]. In the present study, AgNOR dots, both pleomorphic and single, have been counted in cervical smears under different pathological conditions of the uterine cervix, and the data has been analysed to discover the relation between the quantity of the two types of dots with the progression of the disease.…”

Section: Introductionmentioning

confidence: 93%

ecancermedicalscience

Srivastava²,

Bansal³

et al.

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Objective:Nucleolar organizer region (NOR) associated proteins are argyrophilic and visualized by silver stains. AgNOR pleomorphic dots increase in cancer and most researchers have done a common count of single dots. Pleomorphic dots are few and perhaps indicate a more severe prognosis. The present study was aimed at investigating the relative preponderance and diagnostic value of both pleomorphic and single AgNOR dots in cervical carcinogenesis. Study design:Silver nitrate staining was performed in 50 cervical smears each of cytologically diagnosed normal, inflammatory, low grade squamous intraepithelial lesion (LSIL), High-grade squamous intraepithelial lesion (HSIL), and squamous cell carcinoma cases registered at C.S.M. Medical University, Lucknow, India.Results:The accumulated data revealed a positive and significant correlation of cell counts of both pleomorphic (r = 0.94; p < 0.01) and single dots (r = 0.95; p < 0.01) with disease severity. The rate of increase in cell counts of pleomorphic dots (β = 2.61) was 1.1 times higher than the rate of increase in cell counts of single dots (β = 2.29).Conclusion:This study indicates the diagnostic potential of pleomorphic dots in the process of cervical carcinogenesis. The number of pleomorphic dots also varies significantly in different types of SIL, which may help in discriminating precancerous lesions of the cervix.

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AgNOR polymorphism association with squamous intraepithelial lesions and invasive carcinoma with HPV infection

Abstract: AgNOR polymorphism association with squamous intraepithelial lesions and invasive carcinoma with HPV infection.

Cited by 13 publications

References 26 publications

A Study on the Nucleolar Organizer Regions-As a Tumour Proliferative Marker in Cervical Smears

A Study on the Nucleolar Organizer Regions-As a Tumour Proliferative Marker in Cervical Smears

Comparison of Ki67 and modified AgNOR in dysplastic epithelial lesion, primary squamous cell carcinoma and metastatic squamous cell carcinoma of head and neck

ecancermedicalscience

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